Crucial roles of membrane stability and its related proteins in the tolerance of peach fruit to chilling injury

Abstract

Proteome patterns in peach fruit (Prunus persica L.) stored at different low temperatures were examined in order to gain a better understanding why peach fruit is less prone to chilling injury when stored at 0 degrees C than at 5 degrees C. Some differently expressed proteins in peach fruit stored at 0 and 5 degrees C were identified using electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Among these proteins, four membrane stability related proteins, i.e., enolase, temperature-induced lipocalin, major allergen Pru p 1, and type II SK2 dehydrin were enhanced, but three proteins related to phenolic compounds metabolization, cinnamyl-alcohol dehydrogenase 5, cinnamyl-alcohol dehydrogenase 1, and chorismate mutase, were repressed in peach fruit at 0 degrees C as compared to that at 5 degrees C. The abundance of glucose-6-phosphate dehydrogenase, NADP-dependent isocitrate dehydrogenase, and NADP-dependent malic enzyme, which catalyze the reactions during sugar metabolism and energy pathways, was found to decrease in peach fruit stored at 0 degrees C. In addition, our data revealed that low temperature of 0 degrees C might regulate the endogenous H(2)O(2) level, resulting in activating the transcriptional level of genes encoding the proteins related to membrane stability. These results provide a comprehensive knowledge to understand the mechanisms by which peach fruit stored at 0 degrees C showed a higher chilling tolerance than that at 5 degrees C.

Hot air treatment‐induced arginine catabolism is associated with elevated polyamines and proline levels and alleviates chilling injury in postharvest tomato fruit

“…Similarly, accumulation of Put was also observed in chilling‐injured tomato and pepper fruit, and the induction of Put by heat‐treatment associated with increased resistance to chilling has also been previously reported in several chilling‐sensitive fruit . It is well known that maintenance of membrane stability at low temperature is important for plant resistance to cold stress . Because of the polycationic nature at physiological pH, PAs can bind strongly to anionic sites in cellular components stabilising the membranes and in turn alleviate CI .…”

Transcriptomic Profiling during the Post-Harvest of Heat-Treated Dixiland Prunus persica Fruits: Common and Distinct Response to Heat and Cold

“…By this technique, thaumantin-like proteins [7] and proteins involved in stress response [8] were postulated as playing roles in the protection against CI. Moreover, proteins involved in membrane stability were identified [9], reinforcing the role of lipid unsaturation in the acquisition of chilling tolerance in peach fruit [10], [11].…”

Proteomic responses of fruits to environmental stresses

“…Interestingly, peach fruit is less prone to chilling injury when stored at 0°C than at 5°C (Zhang et al, 2010). Further study using proteomics approach indicated that several membrane stability related proteins were enhanced, while proteins related to phenolic compounds metabolization were repressed in peach fruit at 0°C relative to 5°C.…”

“…Further study using proteomics approach indicated that several membrane stability related proteins were enhanced, while proteins related to phenolic compounds metabolization were repressed in peach fruit at 0°C relative to 5°C. Other proteins involved in sugar metabolism and energy pathways were decreased at 0°C, resulting in the lower assumption of sugars which has several beneficial effects in protecting plants against stresses (Zhang et al, 2010). When stored at LTs, 55 proteins from bell pepper ( Capsicum annuum ) fruits were identified as having differential abundances.…”

“…Several individual groups identified dehydrin protein in peach and citrus fruit stored at LT through traditional 2D-PAGE and difference gel electrophoresis (DIGE) (Nilo et al, 2010; Yun et al, 2010, 2012; Zhang et al, 2010). Other commonly identified proteins by these groups included heat shock protein and dehydrogenase which were well-known to play a key role in plant stress responses (Giannino et al, 2004; Posner et al, 2012).…”