Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.11.040 View full text
Silvia A. Coran, Stefano Mulas, Nadia Mulinacci

Abstract: A new HPTLC method was envisaged to determine rosmarinic acid (RA) in different matrices with the aim of testing the influence of optimizing the main HPTLC operative parameters in view of a more stringent validation process. HPTLC LiChrospher silica gel 60 F254s, 20 cm × 10 cm, plates with toluene:ethyl formate:formic acid (6:4:1, v/v) as the mobile phase were used. Densitometric determinations were performed in reflectance mode at 330 nm. The method was validated giving rise to a dependable and high throughpu…

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