Crosslinked polyacrylamide gel as a dehydrating agent

Abstract

synopsisThe dried crosslinked gel prepared from acrylamide and N, N '-methylenebisacrylamide has been shown to be an efficient drying agent. The effectiveness of the dried gel has been shown to be comparable with that of several inorganic dehydration agents.

Rehydratable gel for rapid loading of nanoliter solution and its application in protein crystallization

“…At low protein concentrations where the appearance of crystals was rare in solution conditions, the presence of the gel resulted in an increased possibility of crystal growth, as in the cases of 60 mg mL 21 lysozyme (Fig. 7a), 50 mg mL 21 and 40 mg mL 21 trypsin (Fig. 7b), 10 mg mL 21 catalase (Fig.…”

“…[17][18][19][20] The dehydrated PA gel possesses a strong affinity to water and has been used as a dehydrating reagent. 21 In our study, PA gel was produced in the microwells and dehydrated for long-term storage. Nanoliter solutions were dispensed into the microwells along the microchannels engraved in the degassed PDMS slab and were simultaneously absorbed by the gel during gel rehydration.…”