Cross-reactivity of pollen allergens: recommendations for immunotherapy vaccines

Weber, Richard W.

doi:10.1097/01.all.0000191240.28255.ab

Cited by 30 publications

(19 citation statements)

References 64 publications

(67 reference statements)

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“…There is ample evidence that both group 1 and group 5 allergens are well conserved among Pooideae, with an amino acid sequence homology in the range of 90 and 55–80%, respectively [13,14,15]. There is some level of conservation of other pollen allergens besides groups 1 and 5 among Pooideae, with homologies at the amino acid level ranging from 40 to 60% (i.e.…”

Section: Arguments In Favour Of a Mixed-pollen Vaccinementioning

confidence: 99%

“…Besides its broad historical utilisation for desensitisation purposes by clinicians, the 5 grass combination can be justified based on (1) the widespread distribution in Europe and North America of these Pooideae, which belong to 2 distinct tribes; (2) a well-characterised content of both major and minor allergens [3, 5,10,11,12,13,14,15]. …”

Section: Which Mixture Of Pooideae Grasses Should We Use?mentioning

confidence: 99%

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Specific Immunotherapy for Common Grass Pollen Allergies: Pertinence of a Five Grass Pollen Vaccine

Moingeon

Hrabina

Bergmann

et al. 2008

Int Arch Allergy Immunol

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Patients throughout Europe are concomitantly exposed to multiple pollens from distinct Pooideae species. Given the overlap in pollination calendars and similar grain morphology, it is not possible to identify which grass species are present in the environment from pollen counts. Furthermore, neither serum IgE reactivity nor skin prick testing allow the identification of which grass species are involved in patient sensitisation. Due to their high level of amino acid sequence homology (e.g., >90% for group 1, 55–80% for group 5), significant cross-immunogenicity is observed between allergens from Pooideae pollens. Nevertheless, pollen allergens also contain species-specific T or B cell epitopes, and substantial quantitative differences exist in allergen (e.g., groups 1 and 5) composition between pollens from distinct grass species. In this context, a mixture of pollens from common and well-characterised Pooideae such as Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Phleum pratense and Poa pratensis is suitable for immunotherapy purposes because (1) it has been validated, both in terms of safety and efficacy, by established clinical practice; (2) it reflects natural exposure and sensitisation conditions; (3) it ensures a consistent and well-balanced composition of critical allergens, thus extending the repertoire of T and B cell epitopes present in the vaccine.

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Section: Arguments In Favour Of a Mixed-pollen Vaccinementioning

confidence: 99%

Section: Which Mixture Of Pooideae Grasses Should We Use?mentioning

confidence: 99%

Specific Immunotherapy for Common Grass Pollen Allergies: Pertinence of a Five Grass Pollen Vaccine

Moingeon

Hrabina

Bergmann

et al. 2008

Int Arch Allergy Immunol

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“…IgE antibody or T-cell receptor, cross-reacting with similar affinity to homologous allergens from different species [1,2,3,4,5]. …”

Section: Introductionmentioning

confidence: 99%

Extensive IgE Cross-Reactivity towards the Pooideae Grasses Substantiated for a Large Number of Grass-Pollen-Sensitized Subjects

Johansen

Weber

Ipsen

et al. 2009

Int Arch Allergy Immunol

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Background: Allergy to taxonomically related species is a common phenomenon caused by the same immunological receptor cross-reacting to homologous allergens from different species. Knowledge of patterns of cross-reactivity is crucial for the selection of optimal products for diagnosis and for specific immunotherapy. The objective of this study was to investigate patterns of serum IgE cross-reactivity towards pollens from various grass species. Methods: With grass group 1 allergens as the representative group, amino acid sequence alignment, structural modelling and comparison of 3D surface characteristics were performed to exemplify the molecular basis of IgE cross-reactivity. IgE binding to extracts from ten different grass species was determined (total number of data pairs >19,000), and IgE inhibition experiments using Phleum pratense were performed. Results: Analysis of surface topography for group 1 grass allergens demonstrated ample space for IgE binding epitopes in surface areas conserved among Pooideae grasses. Significant correlation was observed between the serum IgE response to P. pratense extract and extracts from the other Pooideae grasses analyzed. P. pratense extract was demonstrated to inhibit the binding of IgE to the allergens in all of the extracts included in the investigation, indicating patient IgE to be primarily directed towards common epitopes. Conclusion: Extensive IgE cross-reactivity was observed towards the allergens of the Pooideae grasses, meaning that the immune system does not appear to distinguish based on the IgE level between the different species of this subfamily. The data suggest equal effect upon use of any of the Pooideae species for diagnostic as well as therapeutic purposes.

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“…5). According to previous data, the major antigens in pine pollen are of 42 and 6-8 kDa (Weber 2005). In addition, in the case of Pinus radiata, it has been reported that the main allergens are of 140,85,70,55,42,32,22,19, and 6-8 kDa (Cornford et al 1988;Gastaminza et al 2009).…”

Section: Analysis Of Pine Pollen Proteinmentioning

confidence: 93%

Comparison of IgE induction in mice by pollens from three pine tree species

Kim

Choi

2017

j ecology environ

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Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

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Cross-reactivity of pollen allergens: recommendations for immunotherapy vaccines

Abstract: Characterization of specific pollen allergens and their protein families has provided insight into the grounds for cross-reactivity. Continuing clarification of these relationships will allow the substitution and consolidation of inhalant extracts as described in the conclusion.

Cited by 30 publications

References 64 publications

Specific Immunotherapy for Common Grass Pollen Allergies: Pertinence of a Five Grass Pollen Vaccine

Specific Immunotherapy for Common Grass Pollen Allergies: Pertinence of a Five Grass Pollen Vaccine

Extensive IgE Cross-Reactivity towards the Pooideae Grasses Substantiated for a Large Number of Grass-Pollen-Sensitized Subjects

Comparison of IgE induction in mice by pollens from three pine tree species

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