Cross-reaction between proteins of<i>Larrea divaricata</i>Cav. (jarilla) and proteins of Gram-negative bacteria

Anaya, María Aída Mattar de; Davicino, Roberto; Casali, Yolanda; Correa, Silvia G.; Micalizzi, Blas

doi:10.3109/08923970902971101

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…It has been demonstrated that proteins from the crude aqueous extract of L. divaricata administered with adjuvants to mice can induce a good specific immune response, showing crossreaction with proteins of different Gram-negative bacteria such as Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae and Pseudomonas aeruginosa. In this sense, the IgG and IgA antibodies elicited by immunization with L. divaricata proteins were able to opsonize these bacteria and favor phagocytosis (de Anaya et al, 2009;Sasso et al, 2012;Canale et al, 2018). The anti-L divaricata proteins antiserum can inhibit enzymatic activities of P. aeruginosa such as the hemolytic activity of soluble cell proteins, the protease activity and Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas / 754 extracellular proteins by 100%, 55%-70% and 44% and 95%, respectively (Sasso et al, 2012).…”

Section: Immunomodulatory Effects: Pro-and Antiinflammatory Effectsmentioning

confidence: 99%

Larrea divaricataa native Argentine plant with potential activity against SARS-CoV-2?

Alonso¹,

Marrassini²,

Martin³

et al. 2023

BLACPMA

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Larrea divaricataCav. is an autochthonous South American plant popularly used in inflammatory and infectious diseases with reported anti-inflammatory, immunomodulatory, antimicrobial and antioxidant activities. Covid-19 is an infection caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This virus can cause pneumonia and even death in about 5% of the cases. The objective of the article was to demonstrate, through a literature review, that L. divaricatahas sufficient attributes to be assayed against SARS-CoV-2. For this, the chemical composition, reported activities and docking studies were taken into account. This review demonstrated that the plant extracts are capable of inhibiting the proliferation of fungi, bacteria and viruses and that they exert anti-inflammatory and immunomodulatory actions in different "in vitro" and "in vivo" models. These results suggest that the plant is a good candidate to be studied for the prevention and/or treatment of SARS -CoV-2.

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Section: Immunomodulatory Effects: Pro-and Antiinflammatory Effectsmentioning

confidence: 99%

Larrea divaricataa native Argentine plant with potential activity against SARS-CoV-2?

Alonso¹,

Marrassini²,

Martin³

et al. 2023

BLACPMA

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“…Experimental studies with L. divaricata extracts have demonstrated fungotoxic and antimicrobial activity, (2)(3)(4) antitumoral effects, (5)(6)(7) immunostimulatory effects (8,9) and cross-reaction with proteins of Gramnegative bacteria. (10) Pseudomonas aeruginosa is a Gram-negative bacterium considered as an opportunistic pathogen which can cause severe and lethal infections in vulnerable hosts due to its ability to secrete many extracellular factors that are associated with the virulence. (11) It is a major health problem in hospitals, especially with immunocompromised patients (i.e.…”

Section: Introductionmentioning

confidence: 99%

Neutralization ofPseudomonas aeruginosaenzymatic activity by antibodies elicited with proteins ofLarrea divaricataCav.

Sasso¹,

Anaya²,

Davicino³

et al. 2012

Immunopharmacology and Immunotoxicology

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Larrea divaricata Cav. (Jarilla) is a bush widely used in folk therapy for the treatment of several pathologies. Partially purified proteins of crude extract (JPCE) cross-react with proteins of Gram-negative bacteria, including Pseudomonas aeruginosa, which is an opportunistic pathogen that causes several intrahospitalary infections. This bacterium produces many proteins with enzymatic activity, including hemolysins and proteases that play a major role in acute infection caused by this bacterium. The aim of our work was to investigate if antibodies against with L. divaricata neutralize the hemolytic and proteolytic activity of P. aeruginosa. The hemolytic activity of soluble cellular proteins was inhibited 100% and extracellular proteins (EP) showed an inhibition between 44 and 95% when both bacterial fractions were treated with anti-JPCE serum. Also, in EP the neutralization was directed towards the active site of the hemolysin. When protease activity of extracellular products was tested, bands of 217, 155, 121, 47 and 27 kDa were observed in native zymograms. Neutralization between 55 and 70% of the bands of 217, 155 and 121 kDa was observed when EP were treated with anti-JPCE serum. In conclusion, our data clearly demonstrate that antibodies elicited with L. divaricata' proteins are able to neutralize the hemolytic and proteolytic activity of P. aeruginosa cellular and extracellular proteins. Our study constitutes the first report that associates the immunogenicity of plant proteins and bacterial proteins with enzymatic activity. These findings could be relevant in the development of alternatives therapies for patients suffering intrahospitalary opportunistic infections with P. aeruginosa.

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Immunization with Larrea divaricata Cav. proteins elicits opsonic antibodies against Pseudomonas aeruginosa and induces phagocytic activity of murine macrophages

Canale

Dávila

Sasso

et al. 2018

Microbial Pathogenesis

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Cross-reaction between proteins ofLarrea divaricataCav. (jarilla) and proteins of Gram-negative bacteria

Cited by 6 publications

References 21 publications

Larrea divaricataa native Argentine plant with potential activity against SARS-CoV-2?

Larrea divaricataa native Argentine plant with potential activity against SARS-CoV-2?

Neutralization ofPseudomonas aeruginosaenzymatic activity by antibodies elicited with proteins ofLarrea divaricataCav.

Immunization with Larrea divaricata Cav. proteins elicits opsonic antibodies against Pseudomonas aeruginosa and induces phagocytic activity of murine macrophages

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