2021
DOI: 10.1021/acs.analchem.1c02826
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Abstract: Modern biomarker and translational research as well as personalized health care studies rely heavily on powerful omics’ technologies, including metabolomics and lipidomics. However, to translate metabolomics and lipidomics discoveries into a high-throughput clinical setting, standardization is of utmost importance. Here, we compared and benchmarked a quantitative lipidomics platform. The employed Lipidyzer platform is based on lipid class separation by means of differential mobility spectrometry with subsequen… Show more

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Cited by 42 publications
(39 citation statements)
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“…Importantly, the presented HILIC approach offers the extended coverage of SP and GP species (along with ether-linked species, PI, PS, LPS, and LPS). For comparison, the commonly applied DIMS quantification method using Lipidyzer 18 and one of the most comprehensive RPLC based approaches 4 allow for detecting 737 and 636 unique lipid species in human plasma, respectively. In general, the coverage is dependent on the sample type and its composition 46 .…”
Section: Resultsmentioning
confidence: 99%
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“…Importantly, the presented HILIC approach offers the extended coverage of SP and GP species (along with ether-linked species, PI, PS, LPS, and LPS). For comparison, the commonly applied DIMS quantification method using Lipidyzer 18 and one of the most comprehensive RPLC based approaches 4 allow for detecting 737 and 636 unique lipid species in human plasma, respectively. In general, the coverage is dependent on the sample type and its composition 46 .…”
Section: Resultsmentioning
confidence: 99%
“…The final method in positive mode (for human plasma) has a significantly higher coverage (541 SRM) and therefore lower dwell times compared to independent methods during the initial screen (maximum 309 SRM). As specified above, the extensive coverage of our method is in accordance with the results reported with the Lipidyzer kit, where 704 lipid species belonging to 13 major lipid classes, among which more than 70% TG, were measured with high reproducibility (CV < 20%) in human plasma 18 . Using the presented HILIC approach, the coverage of SP and PL was significantly extended with TG comprising 49% of quantified lipid species.…”
Section: Measurement Precisionmentioning
confidence: 99%
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“…Although such monophasic extractions are in general well suited for lipidomic approaches, the broad nature of the standardized metabolic profiling assay requires a trade-off between coverage, extracted concentration as well as reproducibility across all metabolite classes. The latter criterion was recently highlighted by Ghorasaini et al (2021) in an interlaboratory assessment of extraction protocols for lipidomic analyses. The authors showed that the extraction with MeOH/MTBE performed better and was more practical than the comparable Bligh and Dyer extraction.…”
Section: Discussionmentioning
confidence: 99%