Cross‐Binding of Adenosine by Aptamers Selected Using Theophylline
Po‐Jung Jimmy Huang,
Natasha M. Evans,
Chang Lu
et al.
Abstract:We recently reported that some adenosine binding aptamers can also bind caffeine and theophylline with around 20‐fold lower affinities. This discovery led to the current work to examine the cross‐binding of adenosine to theophylline aptamers. For the DNA aptamer for theophylline, cross‐binding to adenosine was observed, and the affinity was 18 to 38‐fold lower for adenosine based on assays using isothermal titration calorimetry and ThT fluorescence spectroscopy. The binding complexes were characterized using N… Show more
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