Critical Sized Mandibular Defect Regeneration in Preclinical In Vivo Models

Brierly, Gary; Tredinnick, Seamus; Lynham, Anthony; Woodruff, Maria A.

doi:10.1007/s40610-016-0036-4

Cited by 24 publications

(20 citation statements)

References 57 publications

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“…These defects actually represent a suitable model for evaluating the usefulness of treatments, because bone regeneration in critical-sized defects only occurs in the presence of osteogenic materials (32). Experimentally, it was found that the size of the CSD for the dog was 15 mm without periosteum (4,5,32).…”

Section: Discussionmentioning

confidence: 99%

“…Ideal mandibular reconstruction should restore form, function, sensation, mastication efficiency and esthetics (5). Autogenous bone grafting has always been the gold standard in reconstructing critical-sized bone defects; however, it has shown multiple disadvantages, such as increased patient morbidity, the limited volume of transported bone and the need for a second operative site (6).…”

Section: Introductionmentioning

confidence: 99%

“…TE is the application of scientific principles to the design, fabrication, modification, and growth of living tissues using biomaterials, cells and growth factors. The aim of bone tissue engineering is to regenerate lost bone via the use of growth factors and/or cells (5).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Osteoinductive Potential of Freeze-Dried Human Amniotic Membrane (Experimental Study)

Kamal

Ayad

Hassan

et al. 2018

Alexandria Dental Journal

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INTRODUCTION:Bone regeneration represents an important challenge in oral surgery. Several means have been employed to improve bone healing, each having several advantages and disadvantages. The search for new materials and methods is an ongoing process. OBJECTIVES:The aim of the present study was to evaluate the bone inductive effect of the freeze-dried human amniotic membrane (FDAM) as a novel biomaterial for bone regeneration. MATERIALS AND METHODS: Twelve adult male dogs were involved in this study. Bilateral critical-sized mandibular defects were created in each dog. One defect was left uncovered as a control. The other defect was covered with a double layered sterile freeze-dried human amniotic membrane (FDAM) to serve as a study. At each of three time points -4, 8, and 12 weeks -four dogs were euthanized and their mandibles were harvested en bloc and osteotomy sites were submitted for histochemical examination to evaluate bone healing. RESULTS: The tissue samples were obtained after 4, 8, and 12 weeks for histochemical examination. The FDAM was found to enhance the blood supply to the defect area in the study group and gave rise to bone induction (P ˂0.001). CONCLUSIONS: Our study findings indicate that the FDAM has the potential for the enhancement of bone healing and bone induction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Osteoinductive Potential of Freeze-Dried Human Amniotic Membrane (Experimental Study)

Kamal

Ayad

Hassan

et al. 2018

Alexandria Dental Journal

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“…The reconstruction of CSD is a significant problem for Oral and Maxillofacial Surgeons because it will not heal spontaneously without intervention. It can regenerate>10 % of the lost bone during the patient's lifetime [2]. CSD heals predominantly with fibrous tissue, not bone because there are not enough bone-forming cells for bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Demineralized Dentine Material Membrane As Barrier Membrane for Bone Regeneration

Soesilawati¹,

Yuliati²,

Ap³

et al. 2021

Preprint

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Mandibular critical size defect (CSD) due to pathological conditions, trauma, and congenital disease can not heal spontaneously and predominantly filled with fibrous tissue. Therefore, a Guided Bone Regeneration (GBR) combined with bone grafting can be performed. The researchers considered using Demineralized Dentin Material Membrane (DDMM) from bovine dentine as an alternative GBR. This study aimed to determine the amount of fibroblast and collagen density after DDMM and bone graft implantation on CSD. Thirty-six Rattus norvegicus rats were used as samples. Mandibular bone defect 5x5 mm was made, then filled with bone graft and covered with Bovine Pericardium Collagen Membrane (BPCM) in the control group and DDMM in the treatment group. Six samples were sacrificed on 7, 14, and 21 days post-surgical for histology examination. There were no significant differences in the amount of fibroblast and collagen density (p-value > 0,05). The amount of fibroblast is lower and the collagen density is higher in treatment group. DDMM has microporosity to prevent connective tissue ingrowth and dentine tubules to allow growth factors release. DDMM and bone graft implantation can reduce the amount of fibroblasts and increase collagen density of CSD which potentially being used as a CSD alternative treatment for bone regeneration.

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“…These defects primarily arise from tumor resection, infection, physical trauma, and osteomyelitis [2] . Such a critical defect will not heal on its own or regenerate more than 10% of the lost bone within the lifetime of the patient [3] . Not only is mandibular bone important for craniofacial aesthetics, but also for the support of muscles of mastication, facial expression and speech [4] .…”

Section: Introductionmentioning

confidence: 99%

Tissue engineering in mandibular reconstruction: osteogenesis-inducing scaffolds

Nelms¹,

Palmer²

2019

PAR

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Currently, the gold standard for aesthetic and functional reconstruction of critical mandibular defects is an autologous fibular flap; however, this carries risk of donor site morbidity, and is not a promising option in patients with depleted donor sites due to previous surgeries. Tissue engineering presents a potential solution in the design of a biomimetic scaffold that must be osteoconductive, osteoinductive, and support osseointegration. These osteogenesis-inducing scaffolds are most successful when they mimic and interact with the surrounding native macroand micro-environment of the mandible. This is accomplished via the regeneration triad: (1) a biomimetic, bioactive osteointegrative scaffold, most likely a resorbable composite of collagen or a synthetic polymer with collagen-like properties combined with beta-tri calcium phosphate that is 3D printed according to defect morphology; (2) growth factor, most frequently bone morphogenic protein 2 (BMP-2); and (3) stem cells, most commonly bone marrow mesenchymal stem cells. Novel techniques for scaffold modification include the use of nano-hydroxyapatite, or combining a vector with a biomaterial to create a gene activated matrix that produces proteins of interest (typically BMP-2) to support osteogenesis. Here, we review the current literature in tissue engineering in order to discuss the success of varying use and combinations of scaffolding materials (i.e., ceramics, biological polymers, and synthetic polymers) with stem cells and growth factors, and will examine their success in vitro and in vivo to induce and guide osteogenesis in mandibular defects.

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Critical Sized Mandibular Defect Regeneration in Preclinical In Vivo Models

Cited by 24 publications

References 57 publications

Evaluation of the Osteoinductive Potential of Freeze-Dried Human Amniotic Membrane (Experimental Study)

Evaluation of the Osteoinductive Potential of Freeze-Dried Human Amniotic Membrane (Experimental Study)

Demineralized Dentine Material Membrane As Barrier Membrane for Bone Regeneration

Tissue engineering in mandibular reconstruction: osteogenesis-inducing scaffolds

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