CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato

Veillet, Florian; Kermarrec, Marie Paule; Chauvin, Laura; Chauvin, Jean Eric; Nogué, Fabien

doi:10.1371/journal.pone.0235942

Cited by 39 publications

(27 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, knockout of miRNA genes in rice [182] showed improved salt tolerance and targeting susceptibility (S) genes for pathogen re-sistance in Solanaceae crop tomato [183]. CRISPR/Cas system also successfully used to target potato genes, such as StIAA2 [184], granule bound starch synthase gene (GBSS) [185], coilin [186], acetolactate synthase genes (StALS1, StALS2) [187], StGBSSI, StDMR6-1 [188], and phytoene desaturase (PDS) [189]. Recently, non-browning potatoes were developed by knocking-out the polyphenol oxidases gene (StPPO2) [190].…”

Section: Genetic Engineering Approach To Improve Salt Tolerance In Potatomentioning

confidence: 99%

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Chourasia

Lal

Tiwari

et al. 2021

Life

112

View full text Add to dashboard Cite

Among abiotic stresses, salinity is a major global threat to agriculture, causing severe damage to crop production and productivity. Potato (Solanum tuberosum) is regarded as a future food crop by FAO to ensure food security, which is severely affected by salinity. The growth of the potato plant is inhibited under salt stress due to osmotic stress-induced ion toxicity. Salinity-mediated osmotic stress leads to physiological changes in the plant, including nutrient imbalance, impairment in detoxifying reactive oxygen species (ROS), membrane damage, and reduced photosynthetic activities. Several physiological and biochemical phenomena, such as the maintenance of plant water status, transpiration, respiration, water use efficiency, hormonal balance, leaf area, germination, and antioxidants production are adversely affected. The ROS under salinity stress leads to the increased plasma membrane permeability and extravasations of substances, which causes water imbalance and plasmolysis. However, potato plants cope with salinity mediated oxidative stress conditions by enhancing both enzymatic and non-enzymatic antioxidant activities. The osmoprotectants, such as proline, polyols (sorbitol, mannitol, xylitol, lactitol, and maltitol), and quaternary ammonium compound (glycine betaine) are synthesized to overcome the adverse effect of salinity. The salinity response and tolerance include complex and multifaceted mechanisms that are controlled by multiple proteins and their interactions. This review aims to redraw the attention of researchers to explore the current physiological, biochemical and molecular responses and subsequently develop potential mitigation strategies against salt stress in potatoes.

show abstract

Section: Genetic Engineering Approach To Improve Salt Tolerance In Potatomentioning

confidence: 99%

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Chourasia

Lal

Tiwari

et al. 2021

Life

112

View full text Add to dashboard Cite

show abstract

“…For example, designing shorter gRNAs or their improved design (Young et al, 2019), lowering intracellular concentration of the Cas-gRNA complex (Pattanayak et al, 2013), expressing specific anti-CRISPR proteins (Hoffmann et al, 2019), or RNP delivery (Svitashev et al, 2016) seem to generally reduce off-target effects. Moreover, the development (and/or the identification) of other CRISPR-associated nucleases has helped to improve efficiency and specificity and reduce off-target effects (Veillet et al, 2020).…”

Section: Alteration Elsewhere In the Genome (Section 422)mentioning

confidence: 99%

Applicability of the EFSA Opinion on site‐directed nucleases type 3 for the safety assessment of plants developed using site‐directed nucleases type 1 and 2 and oligonucleotide‐directed mutagenesis

Naegeli¹,

Bresson²,

Dalmay³

et al. 2020

EFS2

Self Cite

View full text Add to dashboard Cite

The European Commission requested the EFSA Panel on Genetically Modified Organisms (GMO) to assess whether section 4 (hazard identification) and the conclusions of EFSA's Scientific opinion on the risk assessment of plants developed using zinc finger nuclease type 3 technique (ZFN-3) and other site-directed nucleases (SDN) with similar function are valid for plants developed via SDN-1, SDN-2 and oligonucleotide-directed mutagenesis (ODM). In delivering this Opinion, the GMO Panel compared the hazards associated with plants produced via SDN-1, SDN-2 and ODM with those associated with plants obtained via both SDN-3 and conventional breeding. Unlike for SDN-3 methods, the application of SDN-1, SDN-2 and ODM approaches aims to modify genomic sequences in a way which can result in plants not containing any transgene, intragene or cisgene. Consequently, the GMO Panel concludes that those considerations which are specifically related to the presence of a transgene, intragene or cisgene included in section 4 and the conclusions of the Opinion on SDN-3 are not relevant to plants obtained via SDN-1, SDN-2 or ODM as defined in this Opinion. Overall, the GMO Panel did not identify new hazards specifically linked to the genomic modification produced via SDN-1, SDN-2 or ODM as compared with both SDN-3 and conventional breeding. Furthermore, the GMO Panel considers that the existing Guidance for risk assessment of food and feed from genetically modified plants and the Guidance on the environmental risk assessment of genetically modified plants are sufficient but are only partially applicable to plants generated via SDN-1, SDN-2 or ODM. Indeed, those guidance documents' requirements that are linked to the presence of exogenous DNA are not relevant for the risk assessment of plants developed via SDN-1, SDN-2 or ODM approaches if the genome of the final product does not contain exogenous DNA.

show abstract

“…Some of the Cas9 variants including SpCas9-VQR, SpCas9-EQR, Cas9-NG, and xCas9 3.7 with PAM requirements of NGA, NGAG, NG, and NG/GAA/GAT, respectively, have been successfully used in plant species including, Physcomitrella , Arabidopsis, rice, tomato, and potato ( Zhang et al, 2019 ). Furthermore, The Cas9 orthologs from Staphylococcus aureus (SaCas9) and Streptococcus thermophilus (St1Cas9) which recognize PAM sites NNGRRT and NNGGGT, respectively, have also been used successfully in Arabidopsis, potato, tobacco, rice, and citrus with relatively high editing efficiencies ( Steinert et al, 2015 , 2017 ; Kaya et al, 2016 ; Jia et al, 2017 ; Veillet et al, 2020 ).…”

Section: Crispr-cas Nucleases and Variants Expand The Range Of Targetmentioning

confidence: 99%

Advances in Genome Editing With CRISPR Systems and Transformation Technologies for Plant DNA Manipulation

Nadakuduti

Enciso-Rodríguez

2021

Front. Plant Sci.

View full text Add to dashboard Cite

The year 2020 marks a decade since the first gene-edited plants were generated using homing endonucleases and zinc finger nucleases. The advent of CRISPR/Cas9 for gene-editing in 2012 was a major science breakthrough that revolutionized both basic and applied research in various organisms including plants and consequently honored with “The Nobel Prize in Chemistry, 2020.” CRISPR technology is a rapidly evolving field and multiple CRISPR-Cas derived reagents collectively offer a wide range of applications for gene-editing and beyond. While most of these technological advances are successfully adopted in plants to advance functional genomics research and development of innovative crops, others await optimization. One of the biggest bottlenecks in plant gene-editing has been the delivery of gene-editing reagents, since genetic transformation methods are only established in a limited number of species. Recently, alternative methods of delivering CRISPR reagents to plants are being explored. This review mainly focuses on the most recent advances in plant gene-editing including (1) the current Cas effectors and Cas variants with a wide target range, reduced size and increased specificity along with tissue specific genome editing tool kit (2) cytosine, adenine, and glycosylase base editors that can precisely install all possible transition and transversion mutations in target sites (3) prime editing that can directly copy the desired edit into target DNA by search and replace method and (4) CRISPR delivery mechanisms for plant gene-editing that bypass tissue culture and regeneration procedures including de novo meristem induction, delivery using viral vectors and prospects of nanotechnology-based approaches.

show abstract

CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato

Cited by 39 publications

References 31 publications

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Applicability of the EFSA Opinion on site‐directed nucleases type 3 for the safety assessment of plants developed using site‐directed nucleases type 1 and 2 and oligonucleotide‐directed mutagenesis

Advances in Genome Editing With CRISPR Systems and Transformation Technologies for Plant DNA Manipulation

Contact Info

Product

Resources

About