CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta

Abstract

The small crucifer Cardamine hirsuta bears complex leaves divided into leaflets. This is in contrast to its relative, the reference plant Arabidopsis thaliana, which has simple leaves. Comparative studies between these species provide attractive opportunities to study the diversification of form. Here, we report on the implementation of the CRISPR/Cas9 genome editing methodology in C. hirsuta and with it the generation of novel alleles in the RCO gene, which was previously shown to play a major role in the diversification of form between the two species. Thus, genome editing can now be deployed in C. hirsuta, thereby increasing its versatility as a model system to study gene function and evolution.

CRISPR/Cas9‐mediated knockout of a prolyl‐4‐hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection

“…Plants NbpBV113_3 and NbpBV113_4 showed an editing efficiency close to or above 90% in at least four target sites, indicating a high probability of heritability. [ 53 ] These plants were allowed to self‐pollinate and produce seeds.…”