CRISPR/Cas9 genome editing shows the important role of AZC_2928 gene in nitrogen-fixing bacteria of plants

Wang, Xiaojing; Lv, Sang; Liu, Tao; Wei, Jianhong; Qu, Shiyuan; Lu, Yi; Zhang, Junbiao; Oo, Sanda; Zhang, Baohong; Pan, Xiaoping; Liu, Huawei

doi:10.1007/s10142-020-00739-8

Cited by 9 publications

(2 citation statements)

References 59 publications

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“…CRISPR/Cas9 system is also being mentioned in connection with nitrogen fixation research in legumes. Wang et al (2020) knocked out the AZC_2928 gene in Azorhizobium caulinodans with the use of the genome editing tool, CRISPR/Cas9. The results showed that AZC_2928 plays an extremely important role in regulating the formation of chemotaxis and biofilm.…”

Section: Genome Editingmentioning

confidence: 99%

Pea transformation: History, current status and challenges

Ludvíková¹,

Griga²

2022

Czech J. Genet. Plant Breed.

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This review recapitulates the history, important milestones, the current status, and the perspectives of the pea (Pisum sativum L.) transformation as a tool for pea crop breeding. It summarises the developments of the pea transformation from the first methodological experiments to achieving the complete transformation and regeneration of genetically modified (GM) plants, transformation with the first genes of interest (GOI), to recent techniques of targeted genome editing. We show how recent biotechnological methods and genetic engineering may contribute to pea breeding in order to speed up the breeding process and for the creation of new pea cultivars. The focus is laid on genetic engineering which represents an excellent technology to enhance the pea gene pool with genes of interest which are not naturally present in the pea genome. Different methods of pea transformation are mentioned, as well as various GOI that have been used for pea transformation to date, all aimed at improving transgenic pea traits. Tolerance to herbicides or resistance to viruses, fungal pathogens, and insect pests belong, among others, to the pea traits that have already been modulated by methods of genetic engineering. The production of phytopharmaceuticals is also an important chapter in the use of genetically modified peas. We compare different methods of introducing transgenes to peas and also the usage of different selective and reporter genes. The transformation of other major legumes (soybeans, beans) is marginally mentioned. The effect of genetically modified (GM) peas on animal health (feeding tests, allergenicity) is summarised, the potential risks and benefits of pea modification are evaluated and also the prime expectations of GM peas and the real current state of this technology are compared. Unfortunately, this technology or, more precisely, the products created by this technology are under strict (albeit not scientifically-based) legislative control in the European Union.

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Section: Genome Editingmentioning

confidence: 99%

Pea transformation: History, current status and challenges

Ludvíková¹,

Griga²

2022

Czech J. Genet. Plant Breed.

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“…A xenobiotic response element (XRE) family gene containing a DUF433 domain and a set of uncharacterized genes were identified as key HGT genes in Rhizobium etli CFN42 and Rhizobium leguminosarum strain VF39SM by deleting hypothetical genes [ 37 , 38 , 39 ]. The molecular biological methods offered the possibility of deleting potential rgfs to explore and modify crucial genes related to ICE Ac HGT [ 30 , 31 , 32 , 33 , 34 , 40 , 41 ] in order to maintain a minimal ICE Ac genetic tool that can efficiently pack large DNA fragments and transfer interesting functional genes into recipient cells under desired conditions.…”

Section: Introductionmentioning

confidence: 99%

A Novel Module Promotes Horizontal Gene Transfer in Azorhizobium caulinodans ORS571

Chen

et al. 2022

Genes

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Azorhizobium caulinodans ORS571 contains an 87.6 kb integrative and conjugative element (ICEAc) that conjugatively transfers symbiosis genes to other rhizobia. Many hypothetical redundant gene fragments (rgfs) are abundant in ICEAc, but their potential function in horizontal gene transfer (HGT) is unknown. Molecular biological methods were employed to delete hypothetical rgfs, expecting to acquire a minimal ICEAc and consider non-functional rgfs as editable regions for inserting genes related to new symbiotic functions. We determined the significance of rgf4 in HGT and identified the physiological function of genes designated rihF1a (AZC_3879), rihF1b (AZC_RS26200), and rihR (AZC_3881). In-frame deletion and complementation assays revealed that rihF1a and rihF1b work as a unit (rihF1) that positively affects HGT frequency. The EMSA assay and lacZ-based reporter system showed that the XRE-family protein RihR is not a regulator of rihF1 but promotes the expression of the integrase (intC) that has been reported to be upregulated by the LysR-family protein, AhaR, through sensing host’s flavonoid. Overall, a conservative module containing rihF1 and rihR was characterized, eliminating the size of ICEAc by 18.5%. We propose the feasibility of constructing a minimal ICEAc element to facilitate the exchange of new genetic components essential for symbiosis or other metabolic functions between soil bacteria.

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CRISPRi-Mediated Gene Silencing in Biofilm Cycle and Quorum Sensing

Arora

2024

Gene Editing in Plants

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CRISPR/Cas9 genome editing shows the important role of AZC_2928 gene in nitrogen-fixing bacteria of plants

Cited by 9 publications

References 59 publications

Pea transformation: History, current status and challenges

Pea transformation: History, current status and challenges

A Novel Module Promotes Horizontal Gene Transfer in Azorhizobium caulinodans ORS571

CRISPRi-Mediated Gene Silencing in Biofilm Cycle and Quorum Sensing

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