CRISPR/Cas9 gene editing in a chicken model: current approaches and applications

Chojnacka-Puchta, Luiza; Sawicka, Dorota

doi:10.1007/s13353-020-00537-9

Cited by 24 publications

(17 citation statements)

References 81 publications

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“…However, it is possible to misexpress genes using the RCAS virus system (Logan and Tabin, 1998) and inhibit genes using RNAi (Krull, 2004). Also, more recently genomic editing techniques using CRISPR/Cas9 have started to be used chicken embryos, allowing targeted gene knockdowns (Williams et al, 2018;Chojnacka-Puchta and Sawicka, 2020). Taken all together, these techniques reinforce the usefulness of the chicken embryo in development, how drugs affect development and also genomic and molecular analyses (Burt et al, 1999;Stern, 2018).…”

Section: The Chicken Embryomentioning

confidence: 99%

From the Farm to the Lab: How Chicken Embryos Contribute to the Field of Teratology

et al. 2021

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Congenital anomalies and its causes, particularly, by external factors are the aim of the field called teratology. The external factors studied by teratology are known as teratogens and can be biological or environmental factors for example, chemicals, medications, recreational drugs, environmental pollutants, physical agents (e.g., X-rays and maternal hyperthermia) and maternal metabolic conditions. Proving the teratogenicity of a factor is a difficult task requiring epidemiology studies as well as experimental teratology evidence from the use of animal models, one of which is the chicken embryo. This model in particular has the advantage of being able to follow development live and in vivo, with rapid development hatching around 21 days, is cheap and easy to manipulate and to observe development. All this allows the chicken embryo to be used in drug screening studies, teratogenic evaluation and studies of mechanisms of teratogenicity. The chicken embryo shares morphological, biochemical and genetic similarities with humans as well as mammalian species, making them ideal to ascertain the actions of teratogens, as well as screen drugs to test for their safety. Pre-clinical trials for new drugs are carried out in rodents and rabbits, however, chicken embryos have been used to screen new compounds or analogs of thalidomide as well as to investigate how some drugs can lead to congenital malformations. Indeed, the chicken embryo has proved valuable in understanding how many congenital anomalies, seen in humans, arise following teratogen exposure. The aim of this review is to highlight the role of the chicken embryo as an experimental model for studies in teratology, exploring its use in drug screening studies, phenotypic evaluation and studies of teratogenic mechanisms of action. Here, we discuss many known teratogens, that have been evaluated using the chicken embryo model including some medicines, such as, thalidomide, valproic acid; recreational drugs including alcohol; environmental influences, such as viruses, specifically ZIKV, which is a newly discovered human teratogen. In addition, we discuss how the chicken embryo has provided insight on the mechanisms of teratogenesis of many compounds and also how this impact on drug safety.

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Section: The Chicken Embryomentioning

confidence: 99%

From the Farm to the Lab: How Chicken Embryos Contribute to the Field of Teratology

et al. 2021

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“…Future investigations should focus on comparison studies for human and avian similarities or discrepancies in order to take the differences into account for interpretation of data. However, the characterization of the chick embryo genome and the development of specific antibodies for blood and lymphatic endothelial cells and stroma components, as well as recent studies suggesting potential applications of gene editing in chickens [192,193] will help to better characterize the interactions between implanted human tissues and chicken tissues.…”

Section: Expert Opinionmentioning

confidence: 99%

Tumor grafted – chick chorioallantoic membrane as an alternative model for biological cancer research and conventional/nanomaterial-based theranostics evaluation

Mapanao

Pei

Sarogni

et al. 2021

Expert Opinion on Drug Metabolism & Toxicology

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Introduction: Advancements in cancer management and treatment are associated with strong preclinical research data, in which reliable cancer models are demanded. Indeed, inconsistent preclinical findings and stringent regulations following the 3Rs principle of reduction, refinement, and replacement of conventional animal models currently pose challenges in the development and translation of efficient technologies. The chick embryo chorioallantoic membrane (CAM) is a system for the evaluation of treatment effects on the vasculature, therefore suitable for studies on angiogenesis. Apart from vascular effects, the model is now increasingly employed as a preclinical cancer model following tumorgrafting procedures. Areas covered: The broad application of CAM tumor model is highlighted along with the methods for analyzing the neoplasm and vascular system. The presented and cited investigations focus on cancer biology and treatment, encompassing both conventional and emerging nanomaterial-based modalities. Expert opinion: The CAM tumor model finds increased significance given the influences of angiogenesis and the tumor microenvironment in cancer behavior, then providing a qualified miniature system for oncological research. Ultimately, the establishment and increased employment of such a model may resolve some of the limitations present in the standard preclinical tumor models, thereby redefining the preclinical research workflow.

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“…When DNA fragments with the homology sequences around the DSB site exist, the exogenous DNA is efficiently inserted into the DSB site by intrinsic DNA repair mechanisms 10,11 . The knock-in technology has been used in various species including non-model organisms 12–15 . Therefore, the CRISPR/Cas9-based genome editing system for Pv11 cells should expand the experimental feasibility of exogenous gene expression and lead to revealing the detailed molecular mechanisms of anhydrobiosis in P. vanderplanki .…”

Section: Introductionmentioning

confidence: 99%

Cas9-mediated Genome Editing Reveals a Significant Contribution of Calcium Signaling Pathways to Anhydrobiosis in Pv11

Miyata

Fuse

Tokumoto

et al. 2020

Preprint

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Pv11 is an insect cell line established from the midge Polypedilum vanderplanki that exhibits an extreme desiccation tolerance known as anhydrobiosis. Pv11 has also an anhydrobiotic ability which is induced by trehalose treatment. Here we report the successful construction of the genome editing system for Pv11 cells and its application for identifying the signaling pathways in the anhydrobiosis. Using the Cas9-mediated gene knock-in system, we established GCaMP3-stably expressing Pv11 cells to monitor intracellular Ca2+ mobilization. Intriguingly, trehalose treatment evoked a transient increase of cytosolic Ca2+ concentration, and further experiments indicated the contribution of the calmodulin - calcineurin - NFAT pathway to the tolerance for trehalose treatment as well as the desiccation tolerance, while the calmodulin - calmodulin Kinase - CREB pathway conferred only the desiccation tolerance on Pv11 cells. Thus, our results show the critical contribution of the trehalose-induced Ca2+ surge to the anhydrobiosis and the temporal different roles of each signaling pathway.

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CRISPR/Cas9 gene editing in a chicken model: current approaches and applications

Cited by 24 publications

References 81 publications

From the Farm to the Lab: How Chicken Embryos Contribute to the Field of Teratology

From the Farm to the Lab: How Chicken Embryos Contribute to the Field of Teratology

Tumor grafted – chick chorioallantoic membrane as an alternative model for biological cancer research and conventional/nanomaterial-based theranostics evaluation

Cas9-mediated Genome Editing Reveals a Significant Contribution of Calcium Signaling Pathways to Anhydrobiosis in Pv11

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