CRISPR-Assisted DNA Detection: A Novel dCas9-Based DNA Detection Technique

Xu, Xinhui; Luo, Tao; Gao, Jinliang; Lin, Na; Li, Weiwei; Xia, Xinyi; Wang, Jinke

doi:10.1089/crispr.2020.0041

Cited by 25 publications

(16 citation statements)

References 46 publications

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“…Since the hairpin was labeled with fluorescein, HCR could produce a fluorescent signal on the bead’s surface. As the hairpin was connected continuously, the fluorescence signal became brighter in the positive direction, thereby making it easier to detect the fluorescence signals to the greatest extent without amplifying the target gene[ 32 ].…”

Section: Clinical Application Of Crispr/cas Technologymentioning

confidence: 99%

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Huang¹,

Zhang²,

Zhu³

et al. 2022

WJCC

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The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) proteins constitute the innate adaptive immune system in several bacteria and archaea. This immune system helps them in resisting the invasion of phages and foreign DNA by providing sequence-specific acquired immunity. Owing to the numerous advantages such as ease of use, low cost, high efficiency, good accuracy, and a diverse range of applications, the CRISPR-Cas system has become the most widely used genome editing technology. Hence, the advent of the CRISPR/Cas technology highlights a tremendous potential in clinical diagnosis and could become a powerful asset for modern medicine. This study reviews the recently reported application platforms for screening, diagnosis, and treatment of different diseases based on CRISPR/Cas systems. The limitations, current challenges, and future prospectus are summarized; this article would be a valuable reference for future genome-editing practices.

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Section: Clinical Application Of Crispr/cas Technologymentioning

confidence: 99%

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Huang¹,

Zhang²,

Zhu³

et al. 2022

WJCC

View full text Add to dashboard Cite

show abstract

“…This was demonstrated in biochemical assays, mammalian cells, and zebrafish embryos. Given the fast expansion of the CRISPR/Cas9 toolbox and the availability of photochemically modified gRNAs, we believe this universal design can be applied to all tools relying on correct recognition of a genomic target via a gRNA, including genome labeling, [31] epigenetic modification, [32] transposition, [33] and in vitro nucleic acid detection [34] . We further envision that this approach will be applicable to other oligonucleotide‐guided machinery beyond CRISPR/Cas9 and Argonaute/siRNA [18a] that utilize RNA or DNA guides, including Cas12a, Cas13, Cas14, and other Argonautes, without the need for complex protein engineering efforts.…”

Section: Figurementioning

confidence: 99%

Optical Control of Base Editing and Transcription through Light‐Activated Guide RNA

et al. 2021

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We developed a method to control gene activation and base editing in mammalian cells and zebrafish embryos via photochemically activated, caged guide RNAs. Four evenly distributed, caged nucleobases are installed in the 5’‐protospacer region of the gRNA to block interaction between the dCas9:gRNA complex and the dsDNA target. Upon light activation, gRNA:dsDNA hybridization is restored and catalytically dead Cas9‐based CRISPR activation or base editing is achieved. Caged gRNAs are customizable in design, offer spatiotemporal control of transcription activation and base editing in both mammalian cells and zebrafish embryos, and preserve the ability to form dCas9:gRNA complexes for enhanced gRNA stability. We hereby offer a versatile tool for conditional control of interactions between dCas9‐based regulators with their programmed genomic loci, thereby facilitating parsing of spatiotemporally complex genetic events, such as transcription regulation and single‐base mutagenesis.

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“…Xiong et al realized the rapid and visual detection of two SARS-CoV-2 genes (ORF1ab and E) in a single test and a single strip at the same time based on CRISPR/Cas9‐Mediated Lateral Flow Assay 48 . There are other detection platforms based on CRISPR-Cas9 49 , 50 , such as CASLFA, which has realized accurate diagnosis of Listeria monocytogenes, genetically modified organisms, and African swine fever virus. The application of Cas9-based SARS-CoV-2 diagnostics are presented in Table 2 .…”

Section: Crispr-cas Based Diagnosis Of Covid-19mentioning

confidence: 99%

COVID-19 one year later: a retrospect of CRISPR-Cas system in combating COVID-19

Zhan¹,

Li²,

Yin³

2021

Int. J. Biol. Sci.

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Coronavirus disease 2019 (COVID-19), an infectious disease caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a persistent global threat. The transmission of SARS-CoV-2 is wide and swift. Rapid detection of the viral RNA and effective therapy are imperative to prevent the worldwide spread of the new infectious disease. Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR)- CRISPR-associated protein (Cas) system is an RNA-directed adaptive immune system, and it has been transformed into a gene editing tool. Applications of CRISPR-Cas system involves in many fields, such as human gene therapy, drug discovery and disease diagnosis. Under the background of COVID-19 pandemic, CRISPR-Cas system shows hidden capacity to fight the emergency in many aspects. This review will focus on the role of gene editing in COVID-19 diagnosis and treatment. We will describe the potential use of CRISPR-Cas-based system in combating COVID-19, from diagnosis to treatment. Furthermore, the limitation and perspectives of this novel technology are also evaluated.

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CRISPR-Assisted DNA Detection: A Novel dCas9-Based DNA Detection Technique

Cited by 25 publications

References 46 publications

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Optical Control of Base Editing and Transcription through Light‐Activated Guide RNA

COVID-19 one year later: a retrospect of CRISPR-Cas system in combating COVID-19

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