Crevicular fluid endothelin‐1 levels in periodontal health and disease

Pradeep, A.R.; Guruprasad, C. N.; Swati, P.; Chowdhry, Shikha

doi:10.1111/j.1600-0765.2007.01026.x

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…On the following day, the site for sample collection was well isolated and supragingival plaque removed while taking care not to make contact with the marginal gingiva. A standardized volume of 3 μl GCF was collected by placing a microcapillary pipette (Sigma-Aldrich, St. Louis, MO, USA) at the entrance of the gingival sulcus (extrasulcular method) 32) . Micro-pipettes contaminated with blood or saliva were excluded from the study.…”

Section: Selection Of Site and Collection Of Gcf Fluidmentioning

confidence: 99%

Influence of Smoking on Interleukin-34 Levels in Gingival Crevicular Fluid and Plasma in Periodontal Health and Disease: A Clinico-biochemical Study

Guruprasad

Pradeep

2018

Bull. Tokyo Dent. Coll.

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Interleukin-34 (IL-34), an alternative ligand for macrophage colony-stimulating factor receptor, plays an important role in osteoclastogenesis. The aim of this study was to analyze the effect of smoking on IL-34 levels in gingival crevicular fluid (GCF) and plasma in individuals with healthy periodontium and chronic generalized periodontitis (CP). A total of 60 individuals ranging in age from 25 to 55 years were enrolled in the study. The participants were divided into 4 groups: Group A, 30 samples (15 GCF and 15 plasma) obtained from 15 non-smokers with healthy periodontium; Group B, 30 samples (15 GCF and 15 plasma) from 15 smokers with healthy periodontium; Group C, 30 samples (15 GCF and 15 plasma) from 15 non-smokers with CP; and Group D, 30 samples (15 GCF and 15 plasma) from 15 smokers with CP. The Gingival Index and probing depth scores, together with the Clinical Attachment Level, were assessed in each group as clinical periodontal parameters. Levels of IL-34 in GCF and plasma were quantified using enzyme linked immunosorbent assay. The results showed that the mean IL-34 concentrations in GCF and plasma were highest in Group D, followed by Group C, Group B, and Group A, and the difference among them was statistically significant (p<0.05). The relatively elevated IL-34 levels observed here in smokers with CP suggest that this cytokine offers a potential inflammatory marker of periodontal disease in smokers.

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Section: Selection Of Site and Collection Of Gcf Fluidmentioning

confidence: 99%

Influence of Smoking on Interleukin-34 Levels in Gingival Crevicular Fluid and Plasma in Periodontal Health and Disease: A Clinico-biochemical Study

Guruprasad

Pradeep

2018

Bull. Tokyo Dent. Coll.

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“…Also, periodontal therapy improves endothelium-dependent vasodilation in normotensive and hypertensive patients [36-38]. Patients with PD present higher levels of endothelin-1 compared with those without PD [39, 40]. A nitric oxide synthase inhibitor level (asymmetric dimethylarginine) associated with endothelial dysfunction was higher in hypertensive patients with PD [41].…”

Section: Pathophysiologic Mechanisms That Could Explain the Associatimentioning

confidence: 99%

Is There an Association between Periodontitis and Hypertension?

Paizan¹,

Vilela-Martin²

2014

CCR

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Cardiovascular diseases are the leading cause of deaths. Also, cardiovascular risk factors start the atherosclerotic process, which leads to cardiovascular diseases. Nowadays, periodontal disease can also be considered another cardiovascular risk factor. It involves inflammatory, immunological and humoral activities, which induce the production of proinflammatory cytokines and the destruction of the epithelium. This allows the entry of endotoxins and exotoxins in the bloodstream, which may contribute to atherogenesis and thromboembolic events. There is also direct invasion of the vessel wall by oral pathogens, triggering an inflammatory response that produces endothelial dysfunction. In hypertension, changes in microcirculation can cause ischemia in the periodontium, which favors periodontal disease. Moreover, endothelial dysfunction promotes the formation of atherosclerotic plaque and the development of lesions in target organs. Periodontitis has also been associated with insulin resistance and a higher risk for the metabolic syndrome, which is characterized by oxidative stress. This seems to act as a common link to explain the relationship between each component of the metabolic syndrome (including hypertension) and periodontitis. This article will discuss clinical and experimental evidence, as well as possible pathophysiologic mechanisms and links involved in the relationship among periodontal disease, hypertension and cardiovascular disease.

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“…The expression of ET-1 was strongly associated in the gingival tissue and endothelial cells during periodontitis 14 . More specifically, a clinical study found that, in gingival crevicular fluid, the ET-1 level increased with the progression of the periodontitis, and also that ET-1 was involved in the regulation of IL-1b expression in gingival tissues 15,16 .…”

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confidence: 99%

Analysis of Endothelin-1 Concentrations in Individuals with Periodontitis

Isola

Polizzi

Alibrandi

et al. 2020

Sci Rep

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Endothelin 1 (ET-1) has been shown to have a key role in homeostasis and function of endothelium and maybe fundamental in the relationship between coronary heart disease (CHD) and periodontitis. In this trial, we assessed the influence on serum and salivary ET-1 levels of gingival health, CHD, periodontitis, or a combination of periodontitis-CHD. Clinical and periodontal parameters, were collected from periodontitis patients (n = 34), CHD patients (n = 34), periodontitis + CHD patients (n = 34), and from healthy patients (n = 34) together with saliva and serum samples. The median concentrations of salivary and serum ET-1 were significantly higher in the CHD patients [serum: 1.4(1.1-1.6) pg/ml; saliva 1.2 (0.9-1.6) µmol/g, p < 0.01] and in the periodontitis + CHD patients [serum: 1.7 (1.2-21.8) pg/ml; salivary 1.4(1-1.6) µmol/g, p < 0.001] respect to periodontitis and control patients. Through a univariate regression analysis, c-reactive protein (CRP) and CHD (both p < 0.001) and periodontitis (p = 0.029) were statistically correlated with ET-1 in serum. The multivariate regression analysis demonstrated that only CRP was the statistically predictor of ET-1 in serum(p < 0.001). The multivariate regression analysis in saliva demonstrated that, regarding ET-1 levels the only predictor were CRP (p < 0.001) and total cholesterol (p = 0.042). The present study evidenced that subjects with CHD and periodontitis plus CHD had higher serum and salivary levels of ET-1 compared to subjects with periodontitis and healthy controls. Moreover, only CRP remained a major predictor of increased ET-1 concentrations in both serum and saliva.

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Crevicular fluid endothelin‐1 levels in periodontal health and disease

Abstract: The results showed that all the gingival crevicular fluid samples were negative for the endothelin-1 molecule. Therefore, endothelin-1 cannot be considered as a potential biomarker of periodontal disease progression.

Cited by 14 publications

References 22 publications

Influence of Smoking on Interleukin-34 Levels in Gingival Crevicular Fluid and Plasma in Periodontal Health and Disease: A Clinico-biochemical Study

Influence of Smoking on Interleukin-34 Levels in Gingival Crevicular Fluid and Plasma in Periodontal Health and Disease: A Clinico-biochemical Study

Is There an Association between Periodontitis and Hypertension?

Analysis of Endothelin-1 Concentrations in Individuals with Periodontitis

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