Crawler, a novel Tc1/mariner-type transposable element in Aspergillus oryzae transposes under stress conditions

Ogasawara, Hironobu; Obata, Hiroshi; Hata, Yutaka; Takahashi, Saori; Gomi, Katsuya

doi:10.1016/j.fgb.2009.02.007

Cited by 33 publications

(25 citation statements)

References 55 publications

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“…We have shown that transposition can be activated by exposing cultures to low temperatures and deactivated again by raising the temperature. Modulation of transposition activity by environmental factors was described recently for the transposable elements Aft1 of A. fumigatus, crawler of A. oryzae, and OPHIO1 of Ophiostoma ulmi (1,16,39). crawler, which shows high levels of sequence identity to impala, is activated in the presence of low millimolar levels of copper sulfate, while both crawler and Aft1 have increased levels of transposition in response to heat shock.…”

Section: Discussionmentioning

confidence: 85%

The Transposon impala Is Activated by Low Temperatures: Use of a Controlled Transposition System To Identify Genes Critical for Viability of Aspergillus fumigatus

Carr¹,

Tuckwell²,

Hey³

et al. 2010

Eukaryot Cell

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Genes that are essential for viability represent potential targets for the development of anti-infective agents. However, relatively few have been determined in the filamentous fungal pathogen Aspergillus fumigatus. A novel solution employing parasexual genetics coupled with transposon mutagenesis using the Fusarium oxysporum transposon impala had previously enabled the identification of 20 essential genes from A. fumigatus; however, further use of this system required a better understanding of the mode of action of the transposon itself. Examination of a range of conditions indicated that impala is activated by prolonged exposure to low temperatures. This newly identified property was then harnessed to identify 96 loci that are critical for viability in A. fumigatus, including genes required for RNA metabolism, organelle organization, protein transport, ribosome biogenesis, and transcription, as well as a number of noncoding RNAs. A number of these genes represent potential targets for much-needed novel antifungal drugs.

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Section: Discussionmentioning

confidence: 85%

The Transposon impala Is Activated by Low Temperatures: Use of a Controlled Transposition System To Identify Genes Critical for Viability of Aspergillus fumigatus

Carr¹,

Tuckwell²,

Hey³

et al. 2010

Eukaryot Cell

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“…Active Class II transposition events have been described for cut-and-paste elements in several fungi, including the plant pathogen Fusarium oxysporum (Daboussi et al, 1991;Daboussi and Langin, 1994;Langin et al, 1995), Aspergillus niger (Glayzer et al, 1995;Nyyssönen et al, 1996) , Aspergillus fumigatus (Hey et al, 2008) and industrial Aspergillus oryzae strains (Ogasawara et al, 2009). Several Tc1/mariner transposons exist in fungi, but their copy numbers are however lower compared to other eukaryotes (Miskey et al, 2005;Kempken and Kück, 1998;Daboussi and Capy, 2003).…”

Section: Introductionmentioning

confidence: 99%

Minos as a novel Tc1/mariner-type transposable element for functional genomic analysis in Aspergillus nidulans

Evangelinos¹,

Anagnostopoulos²,

Karvela-Kalogeraki³

et al. 2015

Fungal Genetics and Biology

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“…Thus, some transposable elements appear to spread through the M. perniciosa genome in accordance with some chromosomal groups. Different strategies could be used to demonstrate transposon activity such as gene inactivation (Daboussi et al, 1992;Langin et al, 1995;Maurer et al, 1997;Gó mez-Gó mez et al, 1999;Ogasawara et al, 2009), detection of new insertions in the genome (Anaya & Roncero, 1996;Mes et al, 2000) or through expression analyses (Okuda et al, 1998;Kaneko et al, 2000;Kito et al, 2003;Rep et al, 2005;Ogasawara et al, 2009). For Boto elements of M. perniciosa, the last two strategies were used to give experimental support to their expression and activity, including (i) variation found in the hybridization profiles in different cultures of isolate CP02 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus

et al. 2013

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Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6-12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C-and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.

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Crawler, a novel Tc1/mariner-type transposable element in Aspergillus oryzae transposes under stress conditions

Cited by 33 publications

References 55 publications

The Transposon impala Is Activated by Low Temperatures: Use of a Controlled Transposition System To Identify Genes Critical for Viability of Aspergillus fumigatus

The Transposon impala Is Activated by Low Temperatures: Use of a Controlled Transposition System To Identify Genes Critical for Viability of Aspergillus fumigatus

Minos as a novel Tc1/mariner-type transposable element for functional genomic analysis in Aspergillus nidulans

Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus

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