Cratoxylum formosum ssp. pruniflorum activates the TRAIL death receptor complex and inhibits topoisomerase I

Nonpunya, Apiyada; Sethabouppha, Benjabhorn; Rufini, Stefano; Weerapreeyakul, Natthida

doi:10.1016/j.sajb.2017.11.003

Cited by 12 publications

(14 citation statements)

References 41 publications

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“…The Annexin V-FITC and PI apoptosis assay kit (BioLegend, San Diego, CA, USA) was used to determine mode of cell death as per previous report [36]. The HepG2 cells (8 × 10 5 cell/mL) were seeded in a 24-well plate and incubated for 24 h. The compounds at a concentration of 1 x IC 50 and 2 x IC 50 were added and incubated for 12 and 24 h. After that, cells were washed with PBS and cold BioLegend’s cell staining buffer followed by staining with Annexin V-FITC and PI and cell were incubated in the dark for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity and Apoptosis Induction of Coumarins and Carbazole Alkaloids from Clausena harmandiana

2019

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Seven compounds, carbazole alkaloids (heptaphylline, 7-methoxyheptaphylline, 7-methoxymukonal) and coumarins (clausarin, dentatin, nordentatin, and xanthoxyletin), were isolated from the root bark of Clausena harmandiana. Antioxidation, cytotoxicity and apoptosis induction were evaluated in vitro. Results showed that clausarin exerted the highest DPPH radical scavenging and 7-methoxymukonal had the highest ferric reducing antioxidant power. In contrary, dentatin was the least DPPH radical scavenger, and heptaphylline was the least reducing antioxidant power. The isolated compounds showed different cytotoxicity. The hepatocellular carcinoma (HepG2) was generally more sensitive to the isolated compounds than lung cancer (SK-LU-1), colon cancer (HCT-116), and noncancerous (Vero) cell lines, respectively. Clausarin possessed the highest cytotoxicity selectively against cancer cell lines tested. 7-Methoxymukonal and 7-methoxyheptaphylline exhibited less cytotoxicity only in HepG2 cells and were inactive in the SK-LU-1 and HCT116 cells. Despite xantoxyletin possessing low antioxidant and low cytotoxic activity, it induced the highest apoptosis percentage with the lowest necrosis percentage of HepG2 cells after 24 h. In conclusion, xantoxyletin primarily show potential anticancer activity. The root bark of C. harmandiana is a good source of bioactive compounds or the lead for the development of new pharmaceutical agent.

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Section: Methodsmentioning

confidence: 99%

Cytotoxicity and Apoptosis Induction of Coumarins and Carbazole Alkaloids from Clausena harmandiana

2019

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“…The alkylating activity of ITCs was determined by 4-( p -nitrobenzyl) pyridine (NBP) assay as previously described (Nonpunya et al ., 2018). ITCs (1 mM) were incubated with NBP (20 mM) in NBP buffer solution (pH 4.0) at 70°C, from 0 to 40 min.…”

Section: Methodsmentioning

confidence: 99%

Alyssin and Iberin in Cruciferous Vegetables Exert Anticancer Activity in HepG2 by Increasing Intracellular Reactive Oxygen Species and Tubulin Depolymerization

Pocasap

Weerapreeyakul

Thumanu

2019

Biomolecules & Therapeutics

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To determine the chemopreventive potential of alyssin and iberin, the in vitro anticancer activities and molecular targets of isothiocyanates (ITCs) were measured and compared to sulforaphane in hepatocellular carcinoma cell HepG2. The SR-FTIR spectra observed a similar pattern vis-à-vis the biomolecular alteration amongst the ITCs-treated cells suggesting a similar mode of action. All of the ITCs in this study cause cancer cell death through both apoptosis and necrosis in concentration dependent manner (20–80 μM). We found no interactions of any of the ITCs studied with DNA. Notwithstanding, all of the ITCs studied increased intracellular reactive oxygen species (ROS) and suppressed tubulin polymerization, which led to cell-cycle arrest in the S and G2/M phase. Alyssin possessed the most potent anticancer ability; possibly due to its ability to increase intracellular ROS rather than tubulin depolymerization. Nevertheless, the structural influence of alkyl chain length on anticancer capabilities of ITCs remains inconclusive. The results of this study indicate an optional, potent ITC (viz., alyssin) because of its underlying mechanisms against hepatic cancer. As a consequence, further selection and development of effective chemotherapeutic ITCs is recommended.

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“…C. glaucum is traditionally used to lower blood pressure, while young leaves are used in cuisine. 9 Pharmacological activities showed by the genus Cratoxylum were cytotoxic, [10][11][12][13] antidiabetic activity, 14 antimalarials, 15,16 antibacterial, 17,18 anti-HIV-1, 19 and antioxidant. [20][21][22][23][24] Information related to the evaluation of antioxidant activity and xanthine oxidase inhibitory activity of C. glaucum is very limited.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Xanthine Oxidase Inhibitory and Antioxidant Activities of Three Organs of Idat (Cratoxylum glaucum Korth.) and Correlation with Phytochemical Cont

Juanda¹,

Fidrianny²,

Wirasutisna³

et al. 2021

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Cratoxylum formosum ssp. pruniflorum activates the TRAIL death receptor complex and inhibits topoisomerase I

Cited by 12 publications

References 41 publications

Cytotoxicity and Apoptosis Induction of Coumarins and Carbazole Alkaloids from Clausena harmandiana

Cytotoxicity and Apoptosis Induction of Coumarins and Carbazole Alkaloids from Clausena harmandiana

Alyssin and Iberin in Cruciferous Vegetables Exert Anticancer Activity in HepG2 by Increasing Intracellular Reactive Oxygen Species and Tubulin Depolymerization

Evaluation of Xanthine Oxidase Inhibitory and Antioxidant Activities of Three Organs of Idat (Cratoxylum glaucum Korth.) and Correlation with Phytochemical Cont

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