COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities

Exploring indoor and outdoor dust as a potential tool for detection and monitoring of COVID-19 transmission

“…Viral RNA was extracted from bulk dust samples using a Qiagen RNeasy Power microbiome extraction kit procedure (Qiagen, Hilden, Germany), as described previously. 9 Triplicates of approximately 50 mg of dust were removed from bulk dust samples using a sterilized spatula, and each replicate was extracted individually in a laminar flow biosafety cabinet. All extraction sets included a blank to detect potential contamination.…”

“…During the COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a crucial aspect of public health surveillance. 2 , 5 , 7 , 8 , 9 , 10 , 11 This approach is non-invasive, cost-effective, and can be implemented in public spaces, making it a potent tool for detecting community-level infection and disease outbreaks more efficiently than traditional testing methods. 8 , 12 WBE can help pinpoint the source of infection, whether within a building or a large community.…”

LSTM-Powered COVID-19 prediction in central Thailand incorporating meteorological and particulate matter data with a multi-feature selection approach

“…Subsequently, a second COVID-19 wave began on December 17, 2020, prompting the government to swiftly implement localized lockdowns and active case detection strategies [ 19 ]. The highest number of cases throughout the first two years was 23,418 in August 2021 [ 18 , 20 , 21 ]. The COVID-19 transmission in Thailand is still active and pervasive today, particularly in the central region of the country [ 21 ].…”

“…The highest number of cases throughout the first two years was 23,418 in August 2021 [ 18 , 20 , 21 ]. The COVID-19 transmission in Thailand is still active and pervasive today, particularly in the central region of the country [ 21 ]. The central area of Thailand has been particularly hard-hit by the virus, recording its peak in August 2021 with 13,352 cases, constituting 57 % of the nationwide cases, as shown in Fig.…”

Fate-and-transport modeling of SARS-CoV-2 for rural wastewater-based epidemiology application benefit

“…In general, rural communities are isolated from environmental laboratory services which can impact their ability to obtain timely results on samples collected [ 14 , 15 ]. There also has been a limited amount of studies that instituted WBE in rural communities [ 13 , [15] , [16] , [17] , [18] , [19] ]. A summary table has been included in Table S1 .…”