Coupling of fully automated chip electrospray to Fourier transform ion cyclotron resonance mass spectrometry for high‐performance glycoscreening and sequencing

Froesch, Martin; Bîndilă, Laura; Baykut, Gökhan; Allen, Mark; Peter‐Katalinić, Jasna; Zamfir, Alina D.

doi:10.1002/rcm.1733

Cited by 46 publications

(45 citation statements)

References 41 publications

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“…The highest sensitivity obtained in this set of experiments was 0.5 pmol/ mL, which represents a value with one order of magnitude higher than the one achieved in a similar study carried out by using capillary ESI infusion in the same Apollo Apex II configuration [32]. For the initiation of the chip spray, the fine tuning on the x-, y-, and z-axes of the chip nozzle toward the FTICR-MS inlet has been reported to be a critical step [50]. In addition, it was shown that a significant [61,62].…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 56%

“…Recently, the first coupling of the NanoMate robot to the FTICR-MS at 9.4 T and sustained off-resonance CID (SORI CID) MS/ MS was reported [50]. The new assembly was optimized in the negative ion mode to combine automated sample delivery on the chip along with maximal sensitivity, ultrahigh-resolution, accurate mass determination provided by the FTICR instrument, and efficient MS/MS in one system.…”

Section: Coupling Of the Fully Automated Chip-based Nanoesi System Tomentioning

confidence: 99%

“…In another study, the newly conceived NanoMate/FTICR coupling was tested for screening and sequencing by sustained off-resonance (SORI)-CID of O-glycosylated peptides and amino acids extracted from urine of a patient suffering from Schindler disease and from urine of a healthy age-matched individual for comparative analyses [50]. The mixtures were dissolved in methanol to different concentrations and transferred onto the glass microtiter plate of the NanoMate robot.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

“…For identification of potential diagnostic marker components, a mixture of O-glycopeptides extracted from urine of an age-matched healthy control person, identically prepared as described before, was subjected to (2)ESIchipNanoMate/FTICR-MS and SORI-CID MS/MS under the same experimental conditions as those from patient urine [50]. The MS pattern was different: in this mixture a reduced number of glycoconjugate species has been found, where those with shorter chains and lower degree of sialylation were dominating.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

“…Nomenclature for assignment of fragment ions is according to [61,62]. Reproduced and adapted with permission from [50].…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

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Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, and planar microchips, embedding a microchannel at the end of which electrospray is generated in-plane, on the edge of the microchip. In the last two years, carbohydrate research greatly benefited from the introduction and implementation of the chip-based MS. In two laboratories the advantages of the chip electrospray in terms of ionization efficiency, sensitivity, reproducibility, quality of data in combination with high mass accuracy, and resolution of detection were systematically explored for several carbohydrate classes: O-and N-glycopeptides, oligosaccharides, gangliosides and glycoprotein-derived O-and N-glycans, and glycopeptides. The current state-of-the-art in interfacing the chip electrospray devices to high-performance MS for carbohydrate analysis, and the particular requirements for method optimization in both positive and negative ion modes are reviewed here. The recent applications of these miniaturized devices and their general potential for glycomic-based surveys are highlighted.

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