Coupling ELISA to smartphones for POCT of chronic and congenital Chagas disease

Schaumburg, Federico; Pujato, Nazarena; Peverengo, Luz María; Marcipar, Iván Sergio

doi:10.1016/j.talanta.2022.124246

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…Rapid diagnostic tests (RDTs) are an easy-to-use alternative to conventional tests with high sensitivity and specificity which can improve the access to diagnosis [ 99 ]. Innovative strategies such as coupling ELISA to smartphones for testing of chronic and congenital CD in primary health care or community settings have also been developed to facilitate early diagnosis and management [ 100 ]. Once a diagnosis is established, a strategy facilitating linkage to care would also be necessary.…”

Section: Screening Strategies and Mitigation Of The Burden Of Under-d...mentioning

confidence: 99%

Chagas Disease in Europe

Gonzalez-Sanz,

Crespillo-Andújar,

Chamorro-Tojeiro

et al. 2023

TropicalMed

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Chagas disease is currently present in many non-endemic countries and remains a neglected tropical disease globally. A review of the literature identified significant gaps and scarcity of updated information from European countries, with most studies reporting data from Spain and Italy. The index of underdiagnosis may be as high as 70%, affecting mainly females of child-bearing age. Standardized screening of fertile, non-pregnant, women from endemic countries and subsequent treatment is considered an essential strategy to control transmission and prevent new cases, yet no uniform legislation for screening risk groups exists. There is heterogeneity in Europe in terms of preventive strategies to avoid transfusion-related transmission of Chagas disease, not necessarily in line with the European directives, with some countries conducting systematic screening for T. cruzi infection in blood donors, whilst others rely on pre-transfusion questionnaires. The growing burden of the infection in resource-rich areas may provide an opportunity for progress in certain aspects of control and prevention. Options for improving screening strategies, management and linkage to care are reviewed.

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Section: Screening Strategies and Mitigation Of The Burden Of Under-d...mentioning

confidence: 99%

Chagas Disease in Europe

Gonzalez-Sanz,

Crespillo-Andújar,

Chamorro-Tojeiro

et al. 2023

TropicalMed

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“…These systems may not only use the camera, but also other attributes of smartphones, such the flashlight, data processing, computing power, result reporting and connectivity, among others. This approach replaces all the hardware required for reading purposes (Schaumburg et al, 2023) and, in some special cases, all the analyzing system is the smartphone (Schaumburg et al, 2022).…”

Section: Smartphone-based Reading Without Add-onsmentioning

confidence: 99%

“…For example, geometric variability has been successfully addressed with on-chip alignment marks (Figure 2). These marks: 1) provide an easy-to-find reference, 2) can help guide the operator using complementary marks in the smartphone screen and 3) allow capture only if the relative positions are within an acceptable range (Schaumburg et al, 2022;Schaumburg et al, 2023). Additionally, marks can be used for perspective correction through software processing (Karlsen and Dong, 2017).…”

Section: Addressing Challenges: Proposed Solutionsmentioning

confidence: 99%

“…For yes/no results, a feature with the threshold value separating the positive and negative values can be included for direct comparison. Also, on-chip features can be used to assess non-uniform illumination: a large uniform area or multiple identical spots can be placed near the unknown, then a metric (e.g., standard deviation) can be used as a measure of uniformity (Schaumburg et al, 2023). For quantitative and semi-quantitative analysis, the features presented above must be complemented with a calibration curve, and/or a mathematical law for interpolation.…”

Section: Addressing Challenges: Proposed Solutionsmentioning

confidence: 99%

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Challenges and proposed solutions for optical reading on point-of-need testing systems

Schaumburg,

Berli

2023

Front. Sens.

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A broad spectrum of application fields, including human and animal health, food safety and environmental monitoring, require performing real time, on-field chemical determinations, a concept known as point-of-need testing (PONT). In the case of infectious diseases, it allows for early diagnosis, which usually enables better treatment options for the patient, and permits early outbreak detection and intervention with proper control measures. PONT solutions are usually developed on microfluidic chips, preferably with no extra equipment, as to lower their cost, ease their delivery and portability. However, equipment-free PONT is not always feasible, since off-chip operations (e.g., pumping or heating) might be required and provided by the so-called analyzers. A typical example is the hardware for optical reading, which is the subject of this perspective article. Firstly, the state-of-the-art is briefly analyzed considering the solutions provided by both academy and industry. Special emphasis is made on the smartphone-based approaches that use no additional hardware (add-ons) for optical reading. Smartphones are the straightforward option, since they can replace a number of operations typically done by analyzers. The issues arising from using smartphones without add-ons are classified into variability sources and technical challenges. Finally, a set of methods for dealing with these challenges is proposed and briefly discussed. Simpler systems will be more easily adopted by more users, for a broader range of application cases, and will let PONT be a more powerful tool for improving disease diagnosis and outbreak management.

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Current trends in digital camera-based bioassays for point-of-care tests

Pohanka

2024

Clinica Chimica Acta

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Coupling ELISA to smartphones for POCT of chronic and congenital Chagas disease

Cited by 5 publications

References 37 publications

Chagas Disease in Europe

Chagas Disease in Europe

Challenges and proposed solutions for optical reading on point-of-need testing systems

Current trends in digital camera-based bioassays for point-of-care tests

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