volume 20, issue 4, P217-219 1995
DOI: 10.1111/j.1472-765x.1995.tb00431.x
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Abstract: Two main sources of error in the conventional stained film cell count method, i.e. errors from imperfect spreading of the suspension and uneven distribution of cells in the film, have been eliminated by counting all the cells in the film. Staining solution and sodium alginate were added to the suspension before spreading over the slide. With a microsyringe, 20-microliters suspension was spread in two bands of ca 1.7 mm x 60 mm each. Then total cells could be counted easily in the x 100 magnification field by m…

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