Cost-Effective Multiplex Fluorescence Detection System for PCR Chip

Abstract

The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 μm. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.

Single-shot multi-channel plasmonic real-time polymerase chain reaction for multi-target point-of-care testing

“…34,35 However, they have some technical limitations such as additional lens attachment, different optical paths, and mechanical replacement of fluorescence detection elements for multi-target diagnostics. 36 Microlens array configurations allow close-up fluorescence imaging and real-time quantification during rapid plasmonic PCR at the handheld level but still require simultaneous multi-color quantification in the small RT-qPCR system. 27,37,38 Here, we report a single-shot multi-channel plasmonic RT-qPCR (mpRT-qPCR) assay with ultrafast nanostructure-driven amplification and multi-target quantification.…”

Label-free DNA quantification using isothermal amplification on an exposed core optical fiber microfluidic platform

“…In the above isothermal amplification methods, as for traditional qPCR, DNA is detected and quantified by monitoring a change in fluorescence intensity. 25–29 This requires fluorescent labelling reagents as well as a fluorescence excitation and detection device, which increases the complexity and is not favorable to the needs of on-site detection.…”

Symmetrical Coupling Multi-Cavity Bandpass Filter for CMOS Compatible Fluorescence Detection

“…D ETECTION of fluorescence with a configuration consisted of excitation light source, micro-fluidic chip, optical filters, and complementary metal-oxide-semiconductor (CMOS) sensors, plays a key role in the quantitative analysis of nucleic acid by polymerase chain reaction (PCR) [1], [2], [3], [4], [5], [6], [7]. In this special era that the COVID-19 has been threatening public health all over the world for almost 3 years since the end of year 2019, a rapid responding, portable, and high Wenbing Li is with the Shanghai Industrial µTechnology Research Institute, Shanghai 201899, China (e-mail: quentinlee08@foxmail.com).…”