2021
DOI: 10.1038/s41467-021-24901-3
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Abstract: High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. The most common high-resolution imaging approaches used in combination with fluorescence imaging are electron microscopy and atomic-force microscopy (AFM), originally developed for solid-state material characterization. AFM routinely resolves atomic steps, ho… Show more

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Cited by 26 publications
(17 citation statements)
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“…The combination of SICM with high-performance optical microscopy is a very promising area . The SICM is integrated with an inverted optical microscope (Figure S23) and can thus be used with many of the recently developed super-resolution microscopy techniques …”
Section: Conclusion and Prospectsmentioning
confidence: 99%
See 1 more Smart Citation
“…The combination of SICM with high-performance optical microscopy is a very promising area . The SICM is integrated with an inverted optical microscope (Figure S23) and can thus be used with many of the recently developed super-resolution microscopy techniques …”
Section: Conclusion and Prospectsmentioning
confidence: 99%
“…33 The SICM is integrated with an inverted optical microscope (Figure S23) and can thus be used with many of the recently developed super-resolution microscopy techniques. 34 The SICM field has developed rapidly in recent years, with many additional measurement capabilities being added, such as sample stiffness, 35 surface charge, 32 and local pH. 36 Combining these developments with time-resolved SICM or high-speed SICM will provide insights into eukaryotic membrane processes with three-dimensional nanometer detail.…”
Section: Conclusion and Prospectsmentioning
confidence: 99%
“…For example, light microscopy can be used to observe the behavior of virtually any cell type, while electron microscopy can reveal subcellular structures at much higher resolution in fixed cells. To take advantage of more than one type of microscopy at a time, cell biologists have developed several techniques to correlate data from multiple imaging modalities (Fernandes, Saavedra‐Villanueva, Margeat, Milhiet, & Costa, 2020; Gómez‐Varela et al., 2020; Navikas et al., 2021; Phillips et al., 2020). For example, Correlative Light and Electron Microscopy (CLEM) allows direct association of light microscopy data (e.g., fluorescent protein localization) with higher‐resolution electron microscopy (reviewed in de Boer, Hoogenboom, & Giepmans, 2015).…”
Section: Commentarymentioning
confidence: 99%
“…Because of the inherent spatial resolution of this domain, such systems are difficult to calibrate and force researchers into using single-system multi-modal imaging (1, 2), with on-stage fixation and labeling protocols (1, 3, 4). On the other hand, multi-system correlative imaging (5, 6, 7, 8, 9) is dependent on finding a transformation between the respective coordinate systems. While differences between the systems may prohibit off-the-shelf registration solutions (10, 11, 12, 13), the introduction of external cues such as markings (6) or calibration beads may influence the sample and obstruct image quality.…”
Section: Introductionmentioning
confidence: 99%
“…On the other hand, multi-system correlative imaging (5, 6, 7, 8, 9) is dependent on finding a transformation between the respective coordinate systems. While differences between the systems may prohibit off-the-shelf registration solutions (10, 11, 12, 13), the introduction of external cues such as markings (6) or calibration beads may influence the sample and obstruct image quality. Ideally, reference points should be derived from as little information as possible to achieve multi-system correlative imaging consistently.…”
Section: Introductionmentioning
confidence: 99%