Correlation of two validated methods for the quantification of naringenin in its solid dispersion: HPLC and UV spectrophotometric methods

Jha, Durgesh K.; Shah, Devanshi S.; Talele, Shwetal R.; Amin, Purnima D.

doi:10.1007/s42452-020-2536-3

Cited by 18 publications

(8 citation statements)

References 29 publications

(30 reference statements)

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“…The samples collected from the in vitro, solubility, apparent solubility, and permeability studies were analyzed using ultra-high-performance liquid chromatography with the diode array detector (UHPLC-DAD) method (Dionex Thermo Fisher Scientific, Waltham, MA, USA). The NAR determination was performed using a Phenomenex-C18 column (250 mm × 4.6 mm; 5 µm) as the stationary phase and methanol and water (70:30, v / v ) as the mobile phase [ 53 ]. The flow rate of the mobile phase was 1.0 mL/min.…”

Section: Methodsmentioning

confidence: 99%

The Systems of Naringenin with Solubilizers Expand Its Capability to Prevent Neurodegenerative Diseases

Stasiłowicz-Krzemień

Gołębiewski

Płazińska

et al. 2022

IJMS

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Background: Naringenin (NAR) is a flavonoid with excellent antioxidant and neuroprotective potential that is limited by its low solubility. Thus, solid dispersions with β-cyclodextrin (β-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD), hydroxypropylmethylcellulose (HPMC), and microenvironmental pH modifiers were prepared. Methods: The systems formation analysis was performed by X-Ray Powder Diffraction (XRPD) and Fourier-transform infrared spectroscopy (FT-IR). Water solubility and dissolution rates were studied with a pH of 1.2 and 6.8. In vitro permeability through the gastrointestinal tract (GIT) and the blood-brain barrier (BBB) was assessed with the parallel artificial membrane permeability assay (PAMPA) assay. The antioxidant activity was studied with the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and cupric ion reducing antioxidant capacity (CUPRAC) assays, while in vitro enzymes studies involved the inhibition of acetylcholinesterase, butyrylcholinesterase, and tyrosinase. For the most promising system, in silico studies were conducted. Results: NAR solubility was increased 458-fold by the solid dispersion NAR:HP-β-CD:NaHCO3 in a mass ratio of 1:3:1. The dissolution rate was elevated from 8.216% to 88.712% in a pH of 1.2 and from 11.644% to 88.843% in a pH of 6.8 (within 3 h). NAR GIT permeability, described as the apparent permeability coefficient, was increased from 2.789 × 10−6 cm s−1 to 2.909 × 10−5 cm s−1 in an acidic pH and from 1.197 × 10−6 cm s−1 to 2.145 × 10−5 cm s−1 in a basic pH. NAR BBB permeability was established as 4.275 × 10−6 cm s−1. The antioxidant activity and enzyme inhibition were also increased. Computational studies confirmed NAR:HP-β-CD inclusion complex formation. Conclusions: A significant improvement in NAR solubility was associated with an increase in its biological activity.

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Section: Methodsmentioning

confidence: 99%

The Systems of Naringenin with Solubilizers Expand Its Capability to Prevent Neurodegenerative Diseases

Stasiłowicz-Krzemień

Gołębiewski

Płazińska

et al. 2022

IJMS

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“…Peaks 13, 14, 18, 29, and 30 presented UV spectral characteristics of flavanones or flavanonols due to their UV spectra profile, exhibiting a major band between 270 and 295 nm and a small secondary band in the range of 310-350 nm [76]. Peak 13 showed an absorption maximum of 285 nm and 322 nm.…”

Section: Flavanones and Flavanonols Derivativesmentioning

confidence: 99%

Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

et al. 2023

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Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims to disclose the anti-aging effect of the essential oil (EO) of Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, as well as of the hydrodistillation residual water (HRW), a discarded by-product of EO hydrodistillation. The phytochemical characterization of EO and HRW was assessed by GC-MS and HPLC-PDA-ESI-MSn, respectively. The DPPH, ABTS, and FRAP assays were used to disclose the antioxidant properties. The anti-inflammatory potential was evaluated using lipopolysaccharide-stimulated macrophages by assessing NO production, iNOS, and pro-IL-1β protein levels. Cell migration was evaluated using the scratch wound assay, and the etoposide-induced senescence was used to assess the modulation of senescence. The EO is mainly characterized by carvacrol, while the HRW is predominantly characterized by rosmarinic acid. The HRW exerts a stronger antioxidant effect in the DPPH and FRAP assays, whereas the EO was the most active sample in the ABTS assay. Both extracts reduce NO, iNOS, and pro-IL-1β. The EO has no effect on cell migration and presents anti-senescence effects. In opposition, HRW reduces cell migration and induces cellular senescence. Overall, our study highlights interesting pharmacological properties for both extracts, EO being of interest as an anti-aging ingredient and HRW relevant in cancer therapy.

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“…Peaks 8 and 9 exhibited similar UV profiles and MS fragmentation. Concerning their UV profiles, both peaks showed a major band around 284 nm and a small secondary band at 328 nm which is characteristic of flavanones or flavanonols [ 47 ]. The molecular ion [M − H] observed at m / z 609 with MS 2 and MS 3 base peaks at m / z 301 that correspond to the release of a deoxyhexosyl-hexoside moiety [M − H − 308] − , characteristic of an O-diglycoside.…”

Section: Resultsmentioning

confidence: 99%

Exploring the Traditional Uses of Thymbra capitata Infusion in Algarve (Portugal): Anti-Inflammatory, Wound Healing, and Anti-Aging

Alves-Silva,

Pedreiro,

Cruz

et al. 2023

Pharmaceuticals

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Inflammation plays a pivotal role in the resolution of infection or tissue damage. In addition, inflammation is considered a hallmark of aging, which in turn compromises wound healing. Thymbra capitata is an aromatic plant, whose infusion is traditionally used as an anti-inflammatory and wound-healing agent. In this study, a T. capitata infusion was prepared and characterized by HPLC-PDA-ESI-MSn and its safety profile determined by the resazurin metabolic assay. The anti-inflammatory potential was revealed in lipopolysaccharide (LPS)-stimulated macrophages by assessing nitric oxide (NO) release and levels of inducible nitric oxide synthase (iNOS) and the interleukin-1β pro-form (pro-IL-1β). Wound-healing capacity was determined using the scratch assay. The activity of senescence-associated β-galactosidase was used to unveil the anti-senescent potential, along with the nuclear accumulation of yH2AX and p21 levels. The antiradical potential was assessed by DPPH and ABTS scavenging assays. The infusion contains predominantly rosmarinic acid and salvianolic acids. The extract decreased NO, iNOS, and pro-IL-1β levels. Interestingly, the extract promoted wound healing and decreased β-galactosidase activity, as well as yH2AX and p21 levels. The present work highlights strong antiradical, anti-inflammatory, and wound healing capacities, corroborating the traditional uses ascribed to this plant. We have described, for the first time for this extract, anti-senescent properties.

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Correlation of two validated methods for the quantification of naringenin in its solid dispersion: HPLC and UV spectrophotometric methods

Cited by 18 publications

References 29 publications

The Systems of Naringenin with Solubilizers Expand Its Capability to Prevent Neurodegenerative Diseases

The Systems of Naringenin with Solubilizers Expand Its Capability to Prevent Neurodegenerative Diseases

Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

Exploring the Traditional Uses of Thymbra capitata Infusion in Algarve (Portugal): Anti-Inflammatory, Wound Healing, and Anti-Aging

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