Correlation of TNF‑α and IL‑10 gene polymorphisms with primary nephrotic syndrome

Abstract

The study explored the correlations of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) gene polymorphisms with susceptibility and the condition of primary nephrotic syndrome. A total of 200 patients with primary nephrotic syndrome in Qilu hospital were collected as disease group, and 200 healthy people were selected as control group. Genomic deoxyribonucleic acids (DNAs) of nucleated cells in the peripheral blood were extracted to detect the gene polymorphisms of TNF-α (rs1799724 and rs1800629) and IL-10 (rs1800872 and rs141219090). Allele distributions at rs1799724 (P=0.003) and rs1800629 (P=0.011) of TNF-α gene and at rs1800872 (P=0.033) of IL-10 gene in disease group were different from those in control group. In the disease group, allele C frequency at rs1799724 and allele A frequency at rs1800629 of TNF-α gene and allele T frequency at rs1800872 of IL-10 gene were higher. There were differences between rs1799724 (P=0.007) and rs1800629 (P=0.002) of TNF-α gene. In addition, there was a difference in the frequency of the dominant model of TNF-α gene rs1800629 between disease group and control group (P=0.035), and the frequency of dominant model GG+GA was remarkably lower in the disease group. Additionally, TT genotype at rs1799724 of TNF-α gene was obviously related to the plasma TNF-α level (P<0.05), and the plasma TNF-α level was significantly increased in disease group. AA genotype at rs141219090 of IL-10 gene had a notable correlation with the plasma IL-10 level (P<0.05), and the plasma IL-10 level in disease group was markedly raised. Additionally, CT genotype at rs1799724 of TNF-α gene was related to the 24-h urine protein level (P=0.035), GG genotype at rs1800872 of IL-10 gene was associated with the plasma albumin level (P=0.031), and GG genotype at rs141219090 was related to the serum creatinine level (P=0.047). TNF-α and IL-10 gene polymorphisms are predominantly correlated with the susceptibility and the condition of primary nephrotic syndrome.

Tumor necrosis factor‐α polymorphism and risk of primary nephrotic syndrome: A case–control study and meta‐analysis

“…Six literatures from five countries were enrolled including China, Egypt, Iran, Romania, and India. 18 , 25 , 26 , 27 , 28 , 29 Table 3 describes the whole data and information. Table 4 describes the whole NOS results.…”

“…Informed consent was obtained from all participants. Detailed demographic characteristics such as age, sex, body mass index (BMI), smoking status, and alcohol Xiao et al 25…”

Transcutaneous auricular vagus nerve stimulation (taVNS) for the treatment of pediatric nephrotic syndrome: a pilot study

“…In a small pediatric study, TNF was shown to be higher during times of relapse (mean 4.11 pg/ml) and lower during remission (2.14 pg/ml) for steroid sensitive nephrotic syndrome, while there was no significant difference in TNF for SRNS children who remained protienuric after treatment (6.13 vs. 5.67 ng/ml) (Weisbach and GBea 2017 ). Further, genomic studies have found polymorphisms in TNF associated with the susceptibility for nephrotic syndrome and transcriptomic studies have shown activation of TNF in children and adults with nephrotic syndrome (Mariani LESea n.d); (Xiao 2020 ). In an animal model, rats infused with TNF developed proteinuria; however, the exact mechanism by which cytokines result in proteinuria is unclear (Lai et al 2007 ).…”

Potential Molecular Mechanisms of Ephedra Herb in the Treatment of Nephrotic Syndrome Based on Network Pharmacology and Molecular Docking

“…TNF is primarily produced by mononuclear macrophages and can prevent the body from the infringement by killing tumor cells. It is also proved to be an important cytokine with a wide range of biological effects, which is connected with the susceptibility and development of immune diseases, including NS [ 49 ].…”