Correlation of serum hyaluronic acid with clinical and radiological severity in primary knee osteoarthritis

Abstract

Objective: To investigate relationship between serum hyaluronic acid (sHA) level and the presence and severity of radiographic knee osteoarthritis (OA) as well as degree of knee pain. Design: A total of 150 subjects (75 controls and 75 cases) were enrolled in this study. Based on the Kellgrene Lawrence (K-L) grade, participants were radiographically classified into three groups: mild (K-L grade1 & 2), moderate (K-L grade 3) and severe (K-L grade 4). The degree of knee pain was quantified by WOMAC knee pain score. sHA levels were compared among the controls & cases and with radiological grading. In addition, the correlation between sHA level and the degree of knee pain was analyzed in each group. Results: In relationship between sHA level and the severity of radiographic knee OA, sHA level of the case group was significantly higher than in the normal group (P< 0.001). Furthermore, sHA level correlated with the severity of radiographic knee OA (r = 0.880). sHA level had positive correlation and significant association with WOMAC knee pain in study groups. (r = 0.750, P<0.001) Conclusion: sHA level has the potential to be useful for the diagnosis and prognosis of the severity of primary knee OA.

Comparative Studies of Hyaluronic Acid Concentration in Normal and Osteoarthritic Equine Joints

“…For the biochemical measurements of HA concentration in equine SF samples, a commercially available, non-species specific ELISA kit (Enzyme-Linked Immunosorbent Assay kit) (TECO Hyaluronic Acid, TECO medical AG ® ) was used. This assay has been used extensively so far for the successful determination of HA concentration in biological fluids [23,[25][26][27][28][29]. The assay is based on a HA-specific binding protein (HABP) and is able to detect HA molecules of different molecular weights.…”