Correlation of Bone Morphogenetic Protein-2 Levels in Serum and Synovial Fluid with Disease Severity of Knee Osteoarthritis

Abstract

BackgroundThis study aimed to investigate the bone morphogenetic protein-2 (BMP-2) levels in serum and synovial fluid (SF) of patients with primary knee osteoarthritis (OA) and to exam its correlation with radiographic and symptomatic severity of the disease.Material/MethodsA total of 37 knee OA patients and 20 healthy controls were enrolled in this study. Knee OA radiographic grading was performed according to the Kellgren-Lawrence (KL) grading system by evaluating X-ray changes observed in anteroposterior knee radiography. Symptomatic severity of the disease was evaluated according to the Western Ontario McMaster University Osteoarthritis Index (WOMAC) scores. BMP-2 levels in serum and SF were determined using enzyme-linked immunosorbent assay.ResultsSerum BMP-2 level in patients with knee OA was higher than that in healthy controls. Knee OA patients with KL grade 4 showed significantly elevated BMP-2 levels in the serum and SF compared with those with KL grade 2 and 3. Knee OA patients with KL grade 3 had significant higher SF levels of BMP-2 than those with KL grade 2. BMP-2 levels in the serum and SF of knee OA patients were both positively correlated with KL grades and WOMAC scores.ConclusionsBMP2 levels in serum and SF were closely related to the radiographic and symptomatic severity of knee OA and may serve as an alternative biochemical parameter to determine disease severity of primary knee OA.

Enhanced BMP-2/BMP-4 ratio in patients with peripheral spondyloarthritis and in cytokine- and stretch-stimulated mouse chondrocytes

“…BMP deregulation has already been observed in SpA patients’ serum; however, to our knowledge, this is the first study assessing BMP levels in SpA patients’ SF. The consistency of our results was validated with respect to previous studies that assessed BMP contents in SF from OA patients [ 31 – 34 ]. Three independent studies have demonstrated that BMP-2 serum concentration was in the range of 100 pg/mL and neatly higher in ankylosing spondylitis patients than in healthy controls [ 14 , 15 , 35 ].…”

Identification of Targets of a New Nutritional Mixture for Osteoarthritis Management Composed by Curcuminoids Extract, Hydrolyzed Collagen and Green Tea Extract

“…BMP-2 expression was significantly greater in OA cartilage than in normal cartilage. Patients with severe X-ray knee OA showed significantly increased BMP-2 levels in the serum and synovial fluid compared with those with moderate OA [ 35 ]. BMP-2 concentrations in the serum and synovial fluid of knee OA patients were closely related to the radiographic and symptomatic severity of knee OA [ 35 ].…”

“…Patients with severe X-ray knee OA showed significantly increased BMP-2 levels in the serum and synovial fluid compared with those with moderate OA [ 35 ]. BMP-2 concentrations in the serum and synovial fluid of knee OA patients were closely related to the radiographic and symptomatic severity of knee OA [ 35 ]. Moreover, an increase in BMP-2 levels in mice resulted in severe aggravation of osteophyte formation [ 36 ].…”

In vitro osteogenic capacity of bone marrow MSCs from postmenopausal women reflect the osseointegration of their cementless hip stems

“…Individual variation in endogenous levels of such factors could contribute to differences in both in vitro properties of MSC as well as in vivo properties of MSCs affecting the healing responses upon THA surgery, although clinical evidences are limited. Increased serum levels of BMP-2 have been found in patients with knee OA compared to healthy controls ( Liu et al, 2015 ). Such variations in BMP-2 levels cannot be ruled out as an underlying contributing factor to the observed differences in OB-capacity and osseointegration in the current study.…”