Correlation Between MIB-1 Staining Index and the Immunoreactivity of p53 Protein in Recurrent and Non-recurrent Meningiomas

Abstract

C o r r e l a t i o n B e t w e e n M I B -1 S t a i n i n g I n d e x a n d t h e I m m u n o r e a c t i v i t y o f p 5 3 P r o t e i n i nR e c u r r e n t a n d N o n -r e c u r r e n t M e n i n g i o m a s Wild type p53 protein has been shown by recent investigations to be involved in the negative regulation of cell proliferation, whereas aberrant p53 protein has lost this negative regulation of cell growth. Wild type pS3 protein, which has a very short half-life, has generally been considered to be undetectable using immunohistochemical methods; however, according to a recent report, wild type p53 protein may accumulate in the nuclei because of a defective ubiquitin pathway. Aberrant pS3 protein has a longer half-life, and thus is visible using immunohistochemical methods. In this study, both the proliferative potential represented by the MIB-1 staining index (SI) and the immunoreactivity of pS3 protein in 51 intracranial meningiomas were studied applying immunohistochemical staining methods to archival paraffin sections.Recurrence of meningiomas is considered to be affected by a variety of clinical, biologic, and histopathologic factors, including the patient's age, sex, tumor location, extent of surgical removal, histopathologic findings, estrogen and progesterone receptors, and receptors for other growth factors. Among these factors, the proliferative potential of meningioma is considered by several investigators to be one of the most important. 1 "" In a previous report, we used an anti-Ki-67 monoclonal antibody, MIB-1, that can detect all proliferative cells in routinely processed paraffin sections, 3,612 and have shown that there is a statistically significant correlation between the MIB-1 staining index (SI) and recurrence of meningi-

Histopathological Analyses of Proliferative Potentials of Intracranial Meningiomas Using Bromodeoxyuridine and MIB-1 Immunohistochemistry

“…Yamasaki et al suggested that high levels of vascular endothelial growth factor (VEGF) expression are significantly related to the recurrence of meningioma, and that VEGF secretion from microscopic residue remaining after surgery may induce neovascularization, which promotes the recurrence of meningioma [55]. As to p53 immunohistochemistry, we found a close relationship among p53 immunoreactivity, MIB-1 SI, and recurrence [33]. Karamitopoulou et al found that a good correlation exists between histological grading, MIB-1 and p53 protein expression in meningiomas [26].…”

Primary Ear and Temporal Bone Meningiomas: A Clinicopathologic Study of 36 Cases with a Review of the Literature

“…The cases that were immunoreactive tended to be strongly reactive, but only in a few nuclei. The overall proliferation index was unrelated to prognosis and therefore does not seem valuable in the workup of ear and temporal bone meningiomas (67)(68)(69).…”

Original article Poly(ADP-ribose) polymerase-1 (PARP1) and p53 labelling index correlates with tumour grade in meningiomas

“…The role of p53 has already been examined in the meningiomas: some of the examinations ended with negative or equivocal findings [43,[48][49][50], but some of them showed a significant correlation between the p53 status and the grade or recurrence of the tumour [4,7,8,19,20,28,37,41,44,46,57]. It is also described that p53 immunopositive cells are more frequent in the perinecrotic areas of post-embolised cases than in preserved parts of the tumour [39].…”