Correlation between Antioxidant Activities and Phenolic Contents of Radix Angelicae Sinensis (Danggui)

Abstract

Radix Angelicae Sinensisis (RAS) is one of the most popular traditional Chinese herbal medicines. In the present study, six RAS extracts (i.e., phenolic extract PE, petroleum ether extract PEE, ethyl acetate extract EAE, absolute ethanol extract AEE, 95% ethanol extract 95 EE, and water extract WE) were prepared and their antioxidant activities measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical), ABTS [2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid diammonium salt)], Reducing power, •O2– and lipid peroxidation assays. In general, PE, PEE and EAE had relatively high antioxidant activity, followed by AEE with moderate activity, as compared with 95 EE and WE that had low activity. Their phenolic contents (including total phenolic, ferulic acid, caffeic acid, same as below) were then determined by HPLC or spectrophotometry. The sequence of phenolic contents was roughly identical with that of antioxidant activity. When the values of 1/IC50 of various antioxidant assays were used to evaluate the level of antioxidant of the RAS extracts, (plot between 1/IC50 values and phenolic contents), the correlation coefficient (R) ranged from 0.642 to 0.941, with an average value of 0.839. Significant positive correlations demonstrated that the antioxidant effects of RAS might generally be considered a result of the presence of the phenolic compounds, especially ferulic acid and caffeic acid.

Phenolic Composition and Biological (Anti Diabetic and Antioxidant) Activities of Different Solvent Extracts of an Endemic Plant (Heliotropium Strigosum)

“…Therefore, in current study, the excellent antioxidant potential of methanol extract might be correlated with higher level of phenolic compounds in it. These results are similar to earlier study, who explored a strong correlation between antioxidant activity and phenolic compounds 29 . …”

Effect of Lactic Acid Fermentation on Total Phenolic Content and Antioxidant Activity of Fig Fruit Juice (Ficus carica)

“…Several studies indeed show the positive correlation between the antioxidant activity and total phenolic content, as in the antioxidant activity of Thermopsis turcica (Aksoy et al 2013), buckwheat (Fagopyrum esculentum Moench) (Sun & Ho 2005), ulam raja' (Cosmos caudatus), 'kesum' (Polygonum minus), 'selom' (Oenanthe javanica), 'pegaga' (Centella asiatica) and 'curry leaf' (Murraya koenigii) (Faujan et al 2009), Radix Angelicae Sinensisis (Li et al 2009), and Solanum tuberosum (Hesam et al 2012). Yet, the other studies demonstrate the contrast result showing the negative correlation between the antioxidant activity and total phenolic content, as in the antioxidant potency of Chenopodium quinoa and Amaranthus spp seeds obtained by using 3 different methods (FRAP, DPPH and carotene bleaching) that negatively correlates with the total phenolic content (Nsimba et al 2008).…”

Spectral Correlations in some Substituted Styryl (<i>E</i>)-4-((2-Oxopropyl)Diazenyl) Benzoic Acids

“…While seeking the multi-regression analysis, these chemical shifts (δ, ppm) were satisfactorily correlated with σ I and σ R constants and F and R Swain Lupton's [30] constants. The generated multi-regression analysis equations are given in (11)(12)(13)(14)(15)(16). …”

“…From these studies, the ground state configuration and the effect of substituents on the molecule have been evaluated [11]. Also the spectroscopic data were applied for predicting the behavior of electrochemical redox potential correlation [12], reaction transition state equilibrium [13], IC 50 value of drug molecule correlations [14], qualitative and quantitative estimation [15], enol-enone tautomerism [16]. Generally chalcones possess important biological activities such as anti-microbial [3], antidepressants [17], antiplosmodial [18], antiaids [19] and insect antifeedant activities [20][21][22].…”