Correlation analysis between the chemical contents and bioactivity for the quality control of Alismatis Rhizoma

Gao, Xiaoxv; Sun, Cheng‐Peng; Yu, Zhenglong; Cang, Jian; Tian, Xiangge; Huo, Xiaokui; Feng, Lei; Liu, Xinguang; Wang, Chao; Zhang, Baojing; Ma, Xiaochi

doi:10.1016/j.apsb.2017.09.004

Cited by 18 publications

(9 citation statements)

References 25 publications

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“…Modern chemical structure studies have shown that prostane‐type tetracyclic triterpene is the main form of AR compounds, and the structure primarily has a chain of eight carbons on C 17. The structure mainly includes alisol A, alisol B, alisol C, alisol D and its derivatives. The structure of the derivatives is mostly 11‐deoxy; 16‐epoxy; 13,17‐epoxy; 11/12‐dehydrated; 11‐deoxy‐13,17‐epoxy; 16‐oxo‐11‐deoxy; 16,23‐epoxy.…”

Section: Resultsmentioning

confidence: 99%

Establishing a rapid classification and identification method for the major triterpenoids of Alisma orientale

Yang

Dong

et al. 2019

Phytochemical Analysis

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Introduction Alismatis Rhizoma (AR) has been widely used to treat various diseases. Its complex chemical composition has caused certain difficulties in the analysis of this traditional Chinese medicine. Therefore, it is necessary to establish a method for the rapid classification and identification of the chemical constituents of AR. Objective This article describes a method for the rapid classification and identification of major triterpenoids in AR. Methodology The samples were analysed by ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS). The assay was performed on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with 0.1% formic acid in water (A), and acetonitrile (B) as mobile phase by gradient elution at a flow rate of 0.3 mL/min. In the positive ion mode, the fragment information was obtained and compared with the characteristic fragments and neutral losses described in the literature. Then, the rapid classification and identification of the chemical components from AR were achieved. Results Finally, 25 triterpene compounds of AR were identified. Conclusions The method established in this study achieved the rapid classification and identification of chemical components in AR, which promotes the development of research methods to study the constituents of traditional Chinese medicine.

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Section: Resultsmentioning

confidence: 99%

Establishing a rapid classification and identification method for the major triterpenoids of Alisma orientale

Yang

Dong

et al. 2019

Phytochemical Analysis

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“…Accurate qualitative and quantitative analysis of target components in TCM is the prerequisites for quality control 7 . Currently, the liquid chromatography–mass spectrometry (LC–MS) 8 , 9 , 10 and gas chromatography−mass spectrometry (GC–MS) 11 are the most popular platforms in pharmaceutical analysis due to best specificity and sensitivity 12 .…”

Section: Introductionmentioning

confidence: 99%

Limitation standard of toxic aconitines in Aconitum proprietary Chinese medicines using on-line extraction electrospray ionization mass spectrometry

Qiu

Wei

Sun

et al. 2020

Acta Pharmaceutica Sinica B

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Development of rapid analytical methods and establishment of toxic component limitation standards are of great importance in quality control of traditional Chinese medicine. Herein, an on-line extraction electrospray ionization mass spectrometry (oEESI-MS) coupled with a novel whole process integral quantification strategy was developed and applied to direct determination of nine key aconitine-type alkaloids in 20 Aconitum proprietary Chinese medicines (APCMs). Multi-type dosage forms ( e.g. , tablets, capsules, pills, granules, and liquid preparation) of APCM could be determined directly with excellent versatility. The strategy has the characteristics of high throughput, good tolerance of matrix interference, small amount of sample (∼0.5 mg) and reagent (∼240 μL) consumption, and short analysis time for single sample (<15 min). The results were proved to be credible by high performance liquid chromatography−mass spectrometry (LC–MS) and electrospray ionization mass spectrometry, respectively. Moreover, the limitation standard for the toxic aconitines in 20 APCMs was established based on the holistic weight toxicity (HWT) evaluation and the Chinese Pharmacopoeia severally, and turned out that HWT-based toxicity evaluation results were closer to the real clinical applications. Hence, a more accurate and reliable APCM toxicity limitation was established and expected to play an important guiding role in clinics. The current study extended the power of ambient MS as a method for the direct quantification of molecules in complex samples, which is commonly required in pharmaceutical analysis, food safety control, public security, and many other disciplines.

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“…The main active ingredients of RA are classified into triterpenoids and sesquiterpenoids [9, 10]. Triterpenoids, such as alisol B 23-acetate, exhibit hypolipidemic, hypoglycaemic, and diuretic effects [2, 6, 7].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESI/APCI-MS/MS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature

Tai

Zou

Zhang

et al. 2019

Journal of Analytical Methods in Chemistry

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Rhizoma Alismatis (RA), widely known as “Ze-Xie” in China, is the tuber of Alisma orientale (Sam.) Juzep (Alismaceae), a Chinese herbal medicine that has been used to treat hyperlipidemia, diabetes, hypertension, dysuria, and inflammation. In this study, a sensitive and reliable method based on an ultra-performance liquid chromatography (UPLC) couple with two ionisation modes, including electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) tandem mass spectrometry (MS), namely, UPLC-ESI/APCI-MS/MS was developed and validated to simultaneously determine 8 triterpenoids (ESI mode) and 2 sesquiterpenoids (APCI mode) in RA. Ten marker compounds were analysed with a Waters’ CORTECS UPLC C18 column (200 mm × 2.1 m, 1.6 μm) and gradient elution with water (contained 0.1% formic) and acetonitrile within 7 min. The established method was validated for linearity, intra- and interday precisions, accuracy, recovery, and stability. The calibration curve for 10 marker compounds showed good linear regression (r > 0.9971). The limits of detection and quantification for analytes were 0.14–1.67 ng/mL and 0.44–5.65 ng/mL, respectively. The relative standard deviations (RSD, %) and accuracy (RE, %) of intra- and interday precisions were less than 3.83% and 1.21% and 3.22% and 1.46%, repeatability and stability for real samples were less than 2.78% and 3.19%, respectively. All recoveries of the 10 marker compounds ranged from 97.24% to 102.49% with RSDs less than 4.05%. The developed method efficiently determined the 10 marker compounds in RA and was subsequently applied to optimise harvest time and crude processing temperature. The result indicated the 90% wilted phase and 70°C (or lower) may be the best harvest time and the processing temperature of RA.

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Correlation analysis between the chemical contents and bioactivity for the quality control of Alismatis Rhizoma

Cited by 18 publications

References 25 publications

Establishing a rapid classification and identification method for the major triterpenoids of Alisma orientale

Establishing a rapid classification and identification method for the major triterpenoids of Alisma orientale

Limitation standard of toxic aconitines in Aconitum proprietary Chinese medicines using on-line extraction electrospray ionization mass spectrometry

Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESI/APCI-MS/MS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature

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