Correlated basal expression of immediate early gene egr1 and tyrosine hydroxylase in zebrafish brain and downregulation in olfactory bulb after transitory olfactory deprivation

Kress, Sigrid; Wullimann, Mario F.

doi:10.1016/j.jchemneu.2012.09.002

Cited by 15 publications

(9 citation statements)

References 79 publications

(109 reference statements)

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“…Finally, we identify the area postrema in line with previous research in zebrafish and other teleosts (Hornby et al, 1987; Morita and Finger, 1987; Hornby and Piekut, 1988; Manso et al, 1993; Ma, 1997; Kaslin and Panula, 2001; Castro et al, 2006b) here as the catecholaminergic dorsal population in the very posterior caudal hindbrain that lies dorsally between vagal lobes and the commissural nuclei of Cajal (see justification in Kress and Wullimann, 2012) and no longer as associated with the vascular lacunae seen in the area of the nucleus of the medial longitudinal fascicle (Wullimann et al, 1996, p. 42).…”

Section: Resultssupporting

confidence: 69%

“…Finally, there is also no colocolization of catecholaminergic (mostly noradrenergic) locus coeruleus or area postrema neurons with islet1 -GFP cell bodies which is in line with their alar plate origin. However, some colocalizing catecholamine neurons are seen close to the vagal motor nucleus (the vagal noradrenergic population, Ma, 1994a,b, 1997; Kress and Wullimann, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Adult islet1 Expression Outlines Ventralized Derivatives Along Zebrafish Neuraxis

2019

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Signals issued by dorsal roof and ventral floor plates, respectively, underlie the major patterning process of dorsalization and ventralization during vertebrate neural tube development. The ventrally produced morphogen Sonic hedgehog (SHH) is crucial for vertebrate hindbrain and spinal motor neuron development. One diagnostic gene for motor neurons is the LIM/homeodomain gene islet1 , which has additional ventral expression domains extending into mid- and forebrain. In order to corroborate motor neuron development and, in particular, to improve on the identification of poorly documented zebrafish forebrain islet1 populations, we studied adult brains of transgenic islet1 -GFP zebrafish (3 and 6 months). This molecular neuroanatomical analysis was supported by immunostaining these brains for tyrosine hydroxylase (TH) or choline acetyltransferase (ChAT), respectively, revealing zebrafish catecholaminergic and cholinergic neurons. The present analysis of ChAT and islet1 -GFP label confirms ongoing adult expression of islet1 in zebrafish (basal plate) midbrain, hindbrain, and spinal motor neurons. In contrast, non-motor cholinergic systems lack islet1 expression. Additional presumed basal plate islet1 positive systems are described in detail, aided by TH staining which is particularly informative in the diencephalon. Finally, alar plate zebrafish forebrain systems with islet1 expression are described (i.e., thalamus, preoptic region, and subpallium). We conclude that adult zebrafish continue to express islet1 in the same brain systems as in the larva. Further, pending functional confirmation we hypothesize that the larval expression of sonic hedgehog ( shh ) might causally underlie much of adult islet1 expression because it explains findings beyond ventrally located systems, for example regarding shh expression in the zona limitans intrathalamica and correlated islet1 -GFP expression in the thalamus.

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Section: Resultssupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Adult islet1 Expression Outlines Ventralized Derivatives Along Zebrafish Neuraxis

2019

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“…Scale bar: 50 μm in P for E-P; 10 μm in insets in H,L,P. Nakashima et al, 2003;Akiba et al, 2009;Kress and Wullimann, 2012;Bepari et al, 2012). We show that ZIF268 induction is severely impaired in the GL of COUP-TFI mutant mice, both in basal condition and following acute odour stimulation, supporting a role for COUP-TFI in the control of OB interneuron responsiveness.…”

Section: Discussionmentioning

confidence: 55%

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

et al. 2013

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COUP-TFI is an orphan nuclear receptor acting as a strong transcriptional regulator in different aspects of forebrain embryonic development. In this study, we investigated COUP-TFI expression and function in the mouse olfactory bulb (OB), a highly plastic telencephalic region in which continuous integration of newly generated inhibitory interneurons occurs throughout life. OB interneurons belong to different populations that originate from distinct progenitor lineages. Here, we show that COUP-TFI is highly expressed in tyrosine hydroxylase (TH)-positive dopaminergic interneurons in the adult OB glomerular layer (GL). We found that odour deprivation, which is known to downregulate TH expression in the OB, also downregulates COUP-TFI in dopaminergic cells, indicating a possible correlation between TH-and COUP-TFI-activitydependent action. Moreover, we demonstrate that conditional inactivation of COUP-TFI in the EMX1 lineage results in a significant reduction of both TH and ZIF268 expression in the GL. Finally, lentiviral vector-mediated COUP-TFI deletion in adult-generated interneurons confirmed that COUP-TFI acts cell-autonomously in the control of TH and ZIF268 expression. These data indicate that COUP-TFI regulates TH expression in OB cells through an activitydependent mechanism involving ZIF268 induction and strongly argue for a maintenance rather than establishment function of COUP-TFI in dopaminergic commitment. Our study reveals a previously unknown role for COUP-TFI in the adult brain as a key regulator in the control of sensory-dependent plasticity in olfactory dopaminergic neurons.

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“…egr-1 -deficient mice seem to be unable to form long-term memories in behavioral tasks, such as olfactory discrimination, while their short-term memory and early-LTP remain intact [ 56 ]. In zebrafish, egr-1 activity seems to be involved in imprinting processes in early life stages and later in kin recognition, especially in the OB, since rather low basal expression levels are found in the Dp [ 57 ]. In summary, egr-1 down-regulation in the OB of DOM males in response to olfactory cues of male conspecifics, suggests a possible role of olfactory modulation on memory consolidation of social odors, but on the other hand, little is known about COx modulation with olfactory social stimuli.…”

Section: Resultsmentioning

confidence: 99%

Social odors conveying dominance and reproductive information induce rapid physiological and neuromolecular changes in a cichlid fish

et al. 2015

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BackgroundSocial plasticity is a pervasive feature of animal behavior. Animals adjust the expression of their social behavior to the daily changes in social life and to transitions between life-history stages, and this ability has an impact in their Darwinian fitness. This behavioral plasticity may be achieved either by rewiring or by biochemically switching nodes of the neural network underlying social behavior in response to perceived social information. Independent of the proximate mechanisms, at the neuromolecular level social plasticity relies on the regulation of gene expression, such that different neurogenomic states emerge in response to different social stimuli and the switches between states are orchestrated by signaling pathways that interface the social environment and the genotype. Here, we test this hypothesis by characterizing the changes in the brain profile of gene expression in response to social odors in the Mozambique Tilapia, Oreochromis mossambicus. This species has a rich repertoire of social behaviors during which both visual and chemical information are conveyed to conspecifics. Specifically, dominant males increase their urination frequency during agonist encounters and during courtship to convey chemical information reflecting their dominance status.ResultsWe recorded electro-olfactograms to test the extent to which the olfactory epithelium can discriminate between olfactory information from dominant and subordinate males as well as from pre- and post-spawning females. We then performed a genome-scale gene expression analysis of the olfactory bulb and the olfactory cortex homolog in order to identify the neuromolecular systems involved in processing these social stimuli.ConclusionsOur results show that different olfactory stimuli from conspecifics’ have a major impact in the brain transcriptome, with different chemical social cues eliciting specific patterns of gene expression in the brain. These results confirm the role of rapid changes in gene expression in the brain as a genomic mechanism underlying behavioral plasticity and reinforce the idea of an extensive transcriptional plasticity of cichlid genomes, especially in response to rapid changes in their social environment.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1255-4) contains supplementary material, which is available to authorized users.

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Correlated basal expression of immediate early gene egr1 and tyrosine hydroxylase in zebrafish brain and downregulation in olfactory bulb after transitory olfactory deprivation

Cited by 15 publications

References 79 publications

Adult islet1 Expression Outlines Ventralized Derivatives Along Zebrafish Neuraxis

Adult islet1 Expression Outlines Ventralized Derivatives Along Zebrafish Neuraxis

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

Social odors conveying dominance and reproductive information induce rapid physiological and neuromolecular changes in a cichlid fish

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