Correction to: Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection

Abstract

An amendment to this paper has been published and can be accessed via the original article.

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“…Seventy microliter of aptamers were intraperitoneally injected every week at a concentration of 1 mg/kg. Successful knockdown of Opn was confirmed by RT-PCR and IHC and reported previously (29).…”

“…Chromogenic RNA in situ hybridization on HIV SF162 infected animals have been described previously (29). RNAscope kits and protocol were purchased from ACD Biosciences.…”

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“…All animal protocols and procedures were reviewed and approved by Johns Hopkins University Animal Care and Use Committee and Institutional Review Board (Protocol # MO17M12). The humanization protocol has been described previously (29), and the schematic of the study is illustrated in Figure 1A. NOD.Cg-Prkdc scid IL2rγ tm1Wjl /SzJ (NSG) (5-8 weeks old) mice were purchased from Jackson Laboratory for breeding colonies.…”

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“…The humanized mice were separated and stratified into four groups of females and males either inhibited for OPN (treated with inhibitory aptamers that block OPN receptor signaling decreasing RNA and protein levels) or not (scrambled aptamers) and injected with HIV or buffer control [Figure 1A, (29)]. Mice were injected intravenously with HIV SF162 (1 × 10 5 tissue culture infectious doses/ml).…”

“…Custom Taqman assay to determine HIV SF162 viral RNA as described previously (29). Briefly, RNA was extracted with QIAamp Viral RNA mini kit (Qiagen) and was reversetranscribed to cDNA.…”