Correction to: Genomic analysis of Leptospira interrogans serovar Paidjan and Dadas isolates from carrier dogs and comparative genomic analysis to detect genes under positive selection

Kurilung, Alongkorn; Keeratipusana, Chantisa; Suriyaphol, Prapat; Hampson, David J.; Prapasarakul, Nuvee

doi:10.1186/s12864-019-5612-6

Cited by 2 publications

(2 citation statements)

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“…Altogether, genetic alterations that affect protein function (i.e., missense mutation) may be linked to long-term adaptation, especially to beneficial mutations that are the consequence of positive or diversifying selection ( Xu et al, 2016 ). This advantageous selection may either enforce augmentation of persistence factors and/or repression of VFs during asymptomatic infection, leading to an inheritable shift in the bacterial population to a less pathogenic state in the host ( Kurilung et al, 2019 ). However, the association of genetic mutations with leptospiral asymptomatic infection in dogs remains to be elucidated in further studies.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis and a Novel Set of Missense Mutation of the Leptospira weilii Serogroup Mini From the Urine of Asymptomatic Dogs in Thailand

2021

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Leptospira weilii belongs to the pathogenic Leptospira group and is a causal agent of human and animal leptospirosis in many world regions. L. weilii can produce varied clinical presentations from asymptomatic through acute to chronic infections and occupy several ecological niches. Nevertheless, the genomic feature and genetic basis behind the host adaptability of L. weilii remain elusive due to limited information. Therefore, this study aimed to examine the complete circular genomes of two new L. weilii serogroup Mini strains (CUDO6 and CUD13) recovered from the urine of asymptomatic dogs in Thailand and then compared with the 17 genomes available for L. weilii. Variant calling analysis (VCA) was also undertaken to gain potential insight into the missense mutations, focusing on the known pathogenesis-related genes. Whole genome sequences revealed that the CUDO6 and CUD13 strains each contained two chromosomes and one plasmid, with average genome size and G+C content of 4.37 Mbp and 40.7%, respectively. Both strains harbored almost all the confirmed pathogenesis-related genes in Leptospira. Two novel plasmid sequences, pDO6 and pD13, were identified in the strains CUDO6 and CUD13. Both plasmids contained genes responsible for stress response that may play important roles in bacterial adaptation during persistence in the kidneys. The core-single nucleotide polymorphisms phylogeny demonstrated that both strains had a close genetic relationship. Amongst the 19 L. weilii strains analyzed, the pan-genome analysis showed an open pan-genome structure, correlated with their high genetic diversity. VCA identified missense mutations in genes involved in endoflagella, lipopolysaccharide (LPS) structure, mammalian cell entry protein, and hemolytic activities, and may be associated with host-adaptation in the strains. Missense mutations of the endoflagella genes of CUDO6 and CUD13 were associated with loss of motility. These findings extend the knowledge about the pathogenic molecular mechanisms and genomic evolution of this important zoonotic pathogen.

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Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis and a Novel Set of Missense Mutation of the Leptospira weilii Serogroup Mini From the Urine of Asymptomatic Dogs in Thailand

2021

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“…Moreover, PHAGE_Acinet_Acj9_NC_014663, PHAGE_Bacill_SP_15_ NC_031245, PHAGE_Synech_S_CAM7_NC_031927, PHAGE_ Sphing_PAU_NC_019521 and PHAGE_Synech_ACG_2014f_ NC_026927 were found in the severe genome. Almost all of the incomplete prophages were similar to other Leptospira species that contained incomplete phages with sizes ranging from 4.1 to 13.8 kb [20]. However, PHAGE_Acinet_Acj9_NC_014663 which was found in the severe strain, is the one multiple-drug resistant species [21].…”

Section: Phage Analysismentioning

confidence: 87%

Comparative genome characterization of Leptospira interrogans from mild and severe leptospirosis patients

et al. 2021

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Leptospirosis is a zoonotic disease caused by spirochetes from the genus Leptospira. In Thailand, Leptospira interrogans is a major cause of leptospirosis. Leptospirosis patients present with a wide range of clinical manifestations from asymptomatic, mild infections to severe illness involving organ failure. For better understanding the difference between Leptospira isolates causing mild and severe leptospirosis, illumina sequencing was used to sequence genomic DNA in both serotypes. DNA of Leptospira isolated from two patients, one with mild and another with severe symptoms, were included in this study. The paired-end reads were removed adapters and trimmed with Q30 score using Trimmomatic. Trimmed reads were constructed to contigs and scaffolds using SPAdes. Cross-contamination of scaffolds was evaluated by ContEst16s. Prokka tool for bacterial annotation was used to annotate sequences from both Leptospira isolates. Predicted amino acid sequences from Prokka were searched in EggNOG and David gene ontology database to characterize gene ontology. In addition, Leptospira from mild and severe patients, that passed the criteria e-value < 10e-5 from blastP against virulence factor database, were used to analyze with Venn diagram. From this study, we found 13 and 12 genes that were unique in the isolates from mild and severe patients, respectively. The 12 genes in the severe isolate might be virulence factor genes that affect disease severity. However, these genes should be validated in further study.

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Correction to: Genomic analysis of Leptospira interrogans serovar Paidjan and Dadas isolates from carrier dogs and comparative genomic analysis to detect genes under positive selection

Abstract: Reference 1. Kurilung A, et al. Genomic analysis of Leptospira interrogans serovar Paidjan and Dadas isolates from carrier dogs and comparative genomic analysis to detect genes under positive selection.

Cited by 2 publications

References 1 publication

Comparative Genomic Analysis and a Novel Set of Missense Mutation of the Leptospira weilii Serogroup Mini From the Urine of Asymptomatic Dogs in Thailand

Comparative Genomic Analysis and a Novel Set of Missense Mutation of the Leptospira weilii Serogroup Mini From the Urine of Asymptomatic Dogs in Thailand

Comparative genome characterization of Leptospira interrogans from mild and severe leptospirosis patients

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