Correction: Temporal Expression of Peripheral Blood Leukocyte Biomarkers in a Macaca fascicularis Infection Model of Tuberculosis; Comparison with Human Datasets and Analysis with Parametric/Non-parametric Tools for Improved Diagnostic Biomarker Identification

Javed, Sajid; Marsay, Leanne; Wareham, Alice; Lewandowski, Kuiama; Williams, Ann; Dennis, Michael; Sharpe, Sally; Vipond, Richard; Silman, Nigel; Kempsell, Karen E.

doi:10.1371/journal.pone.0159783

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(3 citation statements)

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“…Both animals developed steadily progressive TB characterized by spreading and coalescing pulmonary granulomas, tuberculous pneumonia, and mediastinal lymphadenopathy, reaching humane endpoint criteria by 107 days postinfection. This TB disease course is consistent with that reported previously for MCMs following aerosol M. tuberculosis infection (14) and suggests that MCMs are relatively susceptible to M. tuberculosis. Since TB is often a more chronic disease, we wanted to characterize T-cell responses over a longer period of time.…”

Section: Discussionsupporting

confidence: 91%

“…Even with this higher dose of M. tuberculosis, MCMs survived at least 12 weeks, whereas aerosol-infected MCMs survived only ϳ6 weeks (14). We were able to detect peripheral T-cell responses to M. tuberculosis in all animals.…”

Section: Discussionmentioning

confidence: 77%

“…A recent study reported infection of MCMs with a high dose of M. tuberculosis via the aerosol route. TB in these animals rapidly progressed, and these animals were euthanized at 6 weeks postinfection (14). Such a rapid disease course complicates the characterization of epitope-specific T-cell responses.…”

mentioning

confidence: 99%

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Characterization of T Cells Specific for CFP-10 and ESAT-6 in Mycobacterium tuberculosis-Infected Mauritian Cynomolgus Macaques

et al. 2017

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Nonhuman primates can be used to study host immune responses to Mycobacterium tuberculosis. Mauritian cynomolgus macaques (MCMs) are a unique group of animals that have limited major histocompatibility complex (MHC) genetic diversity, such that MHC-identical animals can be infected with M. tuberculosis. Two MCMs homozygous for the relatively common M1 MHC haplotype were bronchoscopically infected with 41 CFU of the M. tuberculosis Erdman strain. Four other MCMs, which had at least one copy of the M1 MHC haplotype, were infected with a lower dose of 3 CFU M. tuberculosis. All animals mounted similar T-cell responses to CFP-10 and ESAT-6. Two epitopes in CFP-10 were characterized, and the MHC class II alleles restricting them were determined. A third epitope in CFP-10 was identified but exhibited promiscuous restriction. The CFP-10 and ESAT-6 antigenic regions targeted by T cells in MCMs were comparable to those seen in cases of human M. tuberculosis infection. Our data lay the foundation for generating tetrameric molecules to study epitope-specific CD4 T cells in M. tuberculosis-infected MCMs, which may guide future testing of tuberculosis vaccines in nonhuman primates.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 77%