Correction: Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice

Zhang, Jinjin; Lai, Zhiwen; Shi, Liangyan; Tian, Yifei; Luo, Aiyue; Xu, Zhengfu; Ma, Xiangyi; Wang, Shixuan

doi:10.18632/aging.101569

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aging gives rise to many degenerative skeletal diseases, including OP and other bone diseases. 23 OP, a systemic and multifactorial bone disease resulting in morbidity and mortality among the elderly, is increased year-by-year. 24 Because of fragility, bone fractures become the most common clinical symptom of OP, thus making the elderly at risk for chronic pain and having difficulty of moving.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The role of resveratrol in bone marrow‐derived mesenchymal stem cells from patients with osteoporosis

Xin

Cai

2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

The aim of the present study was to investigate the effects of resveratrol on BMSCs from patients with osteoporosis. The cell viability and proliferation of BMSCs after treatment with different concentrations of resveratrol was respectively observed by MTT assay and EdU staining. The apoptosis was assessed using by TUNEL staining and the pluripotency was analyzed by quantitative reverse transcription‐PCR (qRT‐PCR). The osteogenic differentiation and adipogenic differentiation were determined by alkaline phosphatase (ALP) staining, alizarin red S (ARS) staining, oil red O (ORO) staining and qRT‐PCR analysis. MTT assay showed that Res at 40, 80, 100 μM markedly improved the cell proliferation of BMSCs from patients with osteoporosis. EdU staining indicated that Res treatment significantly accelerated the proliferation of BMSCs. In addition, the results of TUNEL staining revealed that Res at 40, 80, 100 μM inhibited the osteoporosis‐related apoptosis of BMSCs. qRT‐PCR analysis explored that Res treatment played a positive role in the pluripotency in BMSCs. ALP, ARS staining and qRT‐PCR demonstrated that Res promoted the differentiation of BMSCs into osteoblasts, especially at 80 μM. ORO staining and qRT‐PCR analysis proved that treatment of Res inhibited the adipogenesis of BMSCs isolated from patients with osteoporosis. Our findings suggested that Res can play a vital role in the cell viability, proliferation, apoptosis, pluripotency, osteogenesis and adipogenesis of BMSCs. And Res might be an efficient therapeutic approach for treating patients with osteoporosis.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Aging gives rise to many degenerative skeletal diseases, including OP and other bone diseases . OP, a systemic and multifactorial bone disease resulting in morbidity and mortality among the elderly, is increased year‐by‐year .…”

Section: Discussionmentioning

confidence: 99%

The role of resveratrol in bone marrow‐derived mesenchymal stem cells from patients with osteoporosis

Xin

Cai

2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Previous studies have suggested that consecutive superovulation can harm fertility and fecundity in mice, whose AMH expressions, along with the concentrations of estrogen and progesterone significantly decreased [25]; Results of recent studies also suggest that multiple superovulations affect mitochondrial function in cumulus cells, inducing apoptosis and mitochondrial DNA (mtDNA) damage as well as altering histone modifications in early embryos, so as to decrease ovarian functions, reduce the oocyte and embryo quality and delay embryonic development [8][9][10]. Increased cytoplasmic fragmentation, abnormal mitochondrial distribution, and spindle damage were also observed in oocytes ovulated from mice that underwent superovulation [26].…”

Section: Discussionmentioning

confidence: 96%

He’s Yangchao Recipe Ameliorates Ovarian Oxidative Stress of Aging Mice under Consecutive Superovulation Involving JNK- And P53-Related Mechanism

Zhao

Miao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. To evaluate the effects of He’s Yangchao Recipe (HSYC) on ameliorating ovarian oxidative stress of aging mice under consecutive superovulation. Methods. An 8-month-old C57BL/6 female mouse was chosen to establish an aging model under ovarian hyperstimulation. Mice were randomly separated into four groups: R1 as the control group, R4 as the model group, NR4 with N-acetyl-L-cysteine (NAC) administration, and TR4 with HSYC administration. Oocyte collection, in vitro fertilization, and embryo culture were performed. The serum hormone levels were measured by enzyme-linked immunosorbent assays (ELISA); the reactive oxygen species (ROS) level of oocytes, the number of growing follicles, corpus luteum, ovulated oocytes, and developing embryos at each stage, along with the proportions of fragmented oocytes and abnormal mitochondria in granulosa cells (GCs) and the apoptosis rate of GCs were calculated; the mRNA and protein levels of JNK, P53, BAX were detected by real-time PCR and the Simple Western System. Results. HSYC enhanced estradiol, progesterone, and inhibin-B levels and increased growing follicle and corpus luteum and ovulated egg counts compared to the R4 group ( P < 0.05 ), whereas it decreased the proportions of fragmented oocytes ( P < 0.01 ); Meanwhile, embryos from mice subjected to four superovulation cycles with HSYC treated had a higher hatching potential. The ROS level of oocytes is downregulated by HSYC ( P < 0.01 ) and the percentage of abnormal mitochondrial in ovaries of the TR4 group was also significantly declined compared to the R4 group ( P < 0.05 ); the most TUNEL-positive cells proportion was detected in the R4 group; nevertheless, HSYC effectively attenuated this detrimental effect ( P < 0.05 ). The mRNA and protein expressions of JNK and P53 in ovary tissues were reduced in the TR4 group while these genes were upregulated by repeated superovulation ( P < 0.05 ). Conclusions. HSYC exerted promising effects on promoting the diminished ovarian reserve and decreased oocyte quality induced by both aging and consecutive ovarian superovulation, potentially via the ROS/JNK/p53 pathway.

show abstract

Correction: Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice

Cited by 2 publications

References 0 publications

The role of resveratrol in bone marrow‐derived mesenchymal stem cells from patients with osteoporosis

The role of resveratrol in bone marrow‐derived mesenchymal stem cells from patients with osteoporosis

He’s Yangchao Recipe Ameliorates Ovarian Oxidative Stress of Aging Mice under Consecutive Superovulation Involving JNK- And P53-Related Mechanism

Contact Info

Product

Resources

About