Correction: Immunisation with BCG and recombinant MVA85A induces long‐lasting, polyfunctional <i>Mycobacterium tuberculosis</i>‐specific CD4<sup>+</sup> memory T lymphocyte populations

Beveridge, Natalie E. R.; Price, David A.; Casazza, Joseph P.; Pathan, Ansar A.; Sander, Clare R.; Asher, Tedi E.; Ambrozak, David R.; Precopio, Melissa; Scheinberg, Phillip; Alder, Nicola; Koup, Richard A.; Douek, Daniel C.; Hill, Adrian V. S.; McShane, Helen

doi:10.1002/eji.201190032

Cited by 63 publications

(88 citation statements)

References 15 publications

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“…A role for CD8 ϩ T cells in the protection against TB has been demonstrated (15,23,27,32). Similar to results from an earlier study with Mtb72F/AS02 A (42) and studies with the MVA85a TB vaccine candidate (5,18,31), no vaccine-induced antigen-specific CD8 ϩ T cells could be detected by ICS using a short-term in vitro restimulation.…”

Section: Discussionsupporting

confidence: 79%

“…This is the first study which demonstrates the polyfunctionality of vaccine-induced CD4 ϩ T cells in PPD-negative subjects. Other studies of TB vaccine candidates have demonstrated polyfunctional CD4 ϩ T-cell responses in PPD-positive adults (2,5,18,31). Earlier work showed that the MVA85a TB vaccine candidate was immunogenic in PPD-negative British adults and that the magnitude of the IFN-␥ enzyme-linked immunospot assay response was positively influenced by BCG priming (26).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02_ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

Leroux-Roels

Leroux‐Roels

Ofori-Anyinam

et al. 2010

Clin Vaccine Immunol

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Tuberculosis (TB) is a major cause of illness and death worldwide, causing approximately 1.7 million deaths a year (43). Despite global efforts to control or eradicate the disease, the WHO estimates that in 2008 an estimated 8.9 million to 9.9 million people became infected with Mycobacterium tuberculosis. The situation is compounded by the emergence of multidrug-resistant TB. Since one-third of the world's population is estimated to be latently infected with M. tuberculosis and at possible risk of disease, TB prevention remains one of today's greatest public health challenges. An efficacious vaccination strategy is an essential tool to control TB.M. bovis bacillus Calmette-Guérin (BCG), consisting of attenuated strains of M. bovis, is the only TB vaccine currently available. It effectively prevents meningeal and miliary TB in young children (8) but appears to be ineffective in preventing adult-onset TB (protection rates, 0 to 80%) (10) and pulmonary TB in children.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02_ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

Leroux-Roels

Leroux‐Roels

Ofori-Anyinam

et al. 2010

Clin Vaccine Immunol

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“…[9][10][11][12] Multifunctional CD4 or CD8 T cells are further proposed to represent a correlate of vaccinemediated protection against various infectious diseases. 6,[13][14][15] Although initial studies characterized the expression profile of IFN-g and interleukin (IL)-2, 9-11 more recent studies have expanded the investigation and analyzed more complex functional patterns. 8,12 Along with the functional profile, the proliferative potential of antigen-specific T cells is believed to be a key factor in maintaining or restoring effective antiviral immunity.…”

Section: Introductionmentioning

confidence: 99%

MVA-nef induces HIV-1-specific polyfunctional and proliferative T-cell responses revealed by the combination of short- and long-term immune assays

et al. 2010

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Several vaccination trials are evaluating the modified vaccinia virus Ankara (MVA) as a delivery vector in various clinical settings. In this paper, we present the reevaluation of a therapeutic vaccination trial in human immunodeficiency virus (HIV)-1-infected individuals treated with highly active antiretroviral therapy using MVA-expressing HIV-1 nef. Immunogenicity of MVA-nef was assessed using multicolor flow cytometry. Vaccine-induced polyfunctionality and proliferative capacity, which are associated with nonprogressive HIV-1 infection, were detectable by combining two immune assays. By means of short-term polychromatic intracellular cytokine staining, we observed a significant increase in polyfunctional Nef-specific CD4 T cells expressing interferong, interleukin (IL)-2 and CD154 after vaccination, whereas changes in the quality of CD8 T-cell response could not be observed. Only the additional use of a long-term polychromatic Carboxyfluorescein succinimidyl ester (CFSE)-based proliferation assay revealed vaccine-induced Nef-specific CD8, as well as CD4 T cells with proliferative capacity. The correlation between vaccine-induced IL-2 production by CD4 T cells and the increase in proliferating Nef-specific CD8 T cells suggests a causal link between these two functions. These results highlight the importance of combining sophisticated immunomonitoring tools to unravel concealed effects of immunological interventions and support the use of the poxvirus-derived MVA vector to stimulate highly functional HIV-1-specific T-cell responses. However, the clinical benefit of these functional T cells remains to be determined.

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“…In humans, vaccination with a novel tuberculosis vaccine based on modified Vaccinia virus Ankara expressing Ag85A (MVA85A) induces T cells with complex phenotypic and polyfunctional cytokine-secreting profiles, including a less differentiated group that co-expresses IFN-g, TNF and IL-2, and with the ability to proliferate. Such cells are considered more likely than single cytokine secretors to be associated with protection [10,11]. Another novel TB vaccine, based on a recombinant replicationdeficient adenovirus (Ad35)-expressing TB antigens, also induces a robust polyfunctional CD4 1 T-cell response in adults [12].…”

mentioning

confidence: 99%

Polyfunctional T cells in human tuberculosis

Wilkinson

2010

Eur J Immunol

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Studies of chronic viral infections have highlighted the phenotypic and functional heterogeneity of antigen-specific T cells and suggested that polyfunctional T cells that secrete multiple cytokines and are able to proliferate on encounter with antigen are more likely than single cytokine secretors to represent correlates of protective immunity. These findings have prompted the evaluation of such T-cell responses in chronic bacterial infections, such as tuberculosis (TB). A number of studies in humans suggested that polyfunctional T cells may indeed be involved in mediating protection in TB; however, studies that question these findings are also emerging, including a study published in this issue of the European Journal of Immunology. These differing findings highlight the difficulties of studying human immunity to TB and the need for polyfunctional T cells to be evaluated in longitudinal studies as opposed to case-control analyses.

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Correction: Immunisation with BCG and recombinant MVA85A induces long‐lasting, polyfunctional Mycobacterium tuberculosis‐specific CD4⁺ memory T lymphocyte populations

Cited by 63 publications

References 15 publications

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02_ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02_ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

MVA-nef induces HIV-1-specific polyfunctional and proliferative T-cell responses revealed by the combination of short- and long-term immune assays

Polyfunctional T cells in human tuberculosis

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Correction: Immunisation with BCG and recombinant MVA85A induces long‐lasting, polyfunctional Mycobacterium tuberculosis‐specific CD4+ memory T lymphocyte populations

Cited by 63 publications

References 15 publications

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

MVA-nef induces HIV-1-specific polyfunctional and proliferative T-cell responses revealed by the combination of short- and long-term immune assays

Polyfunctional T cells in human tuberculosis

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Product

Resources

About

Correction: Immunisation with BCG and recombinant MVA85A induces long‐lasting, polyfunctional Mycobacterium tuberculosis‐specific CD4⁺ memory T lymphocyte populations

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02_ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults

Evaluation of the Safety and Immunogenicity of Two Antigen Concentrations of the Mtb72F/AS02_ACandidate Tuberculosis Vaccine in Purified Protein Derivative-Negative Adults