Correction: Braun, T., et al. Expanding the Genetic Code for Site-Directed Spin-Labeling. Int. J. Mol. Sci. 2019, 20, 373

Abstract

The authors wish to make the following two corrections to this paper [...]

Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

“…72,76 For an insight on the spin-label ncAA pairs available, we recommend a recent review at the ref. 77.…”

Integrating Electron Paramagnetic Resonance Spectroscopy and Computational Modeling to Measure Protein Structure and Dynamics

“…For example, reduction‐resistant labels, such as sterically‐shielded nitroxide, [22,108] trityl, [7,24,103–105] and gadolinium labels, [109] have been employed to conduct in‐cell measurements. The in‐cell measurements have also been enabled by endogenous labeling strategies, which incorporate genetically‐encoded, unnatural amino acids for SDSL to either bear a paramagnetic center, such as nitroxide, or a reactive moiety for post‐translational conjugation [23,110] . Photoactivable nitroxides have been developed to label unnatural amino acids as well as nucleic acids [111,112] .…”

In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

“…17 However, for EPR applications, the potential of ncAA-based bioorthogonal labelling is still in its infancy, especially in the context of living cells. 18 A spin labelling scheme linking the ncAA p-Acetyl-L-phenylalanine to a nitroxide was pioneered in 2009, 19 while the labelling of green fluorescent protein (GFP) by azide-alkyne cycloaddition in Escherichia coli (E. coli) represented significant advancements in this field. 20,21 Further refinement of this technique has allowed even for distance measurements inside E. coli cells.…”