Corosolic acid improves erectile function in metabolic syndrome rats by reducing reactive oxygen species generation and increasing nitric oxide bioavailability

Abstract

To investigate the effect of corosolic acid treatment on erectile function in metabolic syndrome induced rat model. Fifty male 3-week-old SD rats were fed a high fat and high sugar diet. Six months later, metabolic variables were determined. Metabolic syndrome induced erectile function (MED) rats were confirmed by an apomorphine test. Then MED rats were treated with corosolic acid daily by oral gavage for 4 weeks. To evaluate erectile function, intracavernosal pressure (ICP)/mean arterial blood pressure (MAP) ratio was measured. Thiobarbituric acid reactive substances assay and dihydroethidium staining were used to assess reactive oxygen species (ROS) level. Protein expressions of gp91 phox and eNOS were examined by western blotting and immunohistochemistry. Fasting blood glucose, body weight, total cholesterol and insulin were markedly increased in metabolic syndrome rats compared with those of the control rats (p < 0.05). The ratios of max ICP/MAP and area under curve (AUC)/MAP was markedly reduced in MED rats compared with the control rats (p < 0.05). The concentration of cyclic guanosine mono-phosphate (cGMP) and the expression of eNOS were significantly decreased in MED rats compared with the control group (p < 0.05). Moreover, ROS level and the expression of gp91 phox were significantly increased in MED rats. Treatment with corosolic acid reversed these changes (each p < 0.05). Corosolic acid reduces the level of ROS, ameliorating endothelial dysfunction and improvement of erectile function in MED rats.

The Cardioprotective Effect of Corosolic Acid in the Diabetic Rats: A Possible Mechanism of the PPAR-γ Pathway

“…It is also known as “phyto-insulin” or “plant insulin” because of its excellent antidiabetic effects [ 20 ]. Various pharmacological studies have shown that corosolic acid has anti-obesity [ 21 ], anti-inflammatory [ 22 ], anti-hyperlipidaemic [ 22 ], anti-viral, and anti-cancer properties [ 23 ]. Based on these considerations, the present study aimed to determine whether corosolic acid has a cardioprotective effect in diabetic rats and to explore the mechanisms involved.…”

Preparation of total triterpenoids from Antrodia cinnamomea fermentation mycelium and their in vitro inhibitory effects on hepatocellular carcinoma

“…ROS play an important role in the body. A lower level of ROS promotes the cellular proliferation, differentiation, tissue regeneration and metabolism, and the high concentrations of ROS induce cell apoptosis and necrosis by oxidative stress (Li et al, 2022). Results of this study showed that, with the increase of TT-ACFM concentration, the fluorescence intensity of ROS gradually increased.…”

Improvement effect of enzymic polypeptide from Paeonia ostii seed meal on protein-energy malnutrition mouse model

“…As a marker, MDA is often used to detect the oxidative stress (Reiter et al, 2014;Ho et al, 2013). There are some antioxidant mechanisms against ROS production and oxidative damage in cells (Li et al, 2022). Cellular antioxidant enzyme, including SOD and CAT, can effectively remove hydrogen peroxide and lipid peroxide, and efficiently detoxify superoxide anion (Mruk et al, 2002).…”