Corneal Endothelial Cell Damage by Free Radicals Associated With Ultrasound Oscillation

Murano, N.; Ishizaki, Masamichi; Satō, Shigeru; Fukuda, Yuh; Takahashi, Hiroshi

doi:10.1001/archopht.126.6.816

Cited by 77 publications

(58 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…52 Laboratory and animal studies have shown that ultrasound power within the anterior chamber causes oxidative stress and cellular injury, with the production of free radicals that are toxic to the corneal endothelium. 53,54 However, the magnitude of this cellular damage in vivo with modern phacoemulsification techniques is uncertain, so its clinical relevance remains unclear.…”

Section: Lens Liquefaction and Fragmentationmentioning

confidence: 99%

The journey to femtosecond laser-assisted cataract surgery: new beginnings or a false dawn?

Trikha

Turnbull

Morris

et al. 2013

Eye

View full text Add to dashboard Cite

Femtosecond laser-assisted cataract surgery (FLACS) represents a potential paradigm shift in cataract surgery, but it is not without controversy. Advocates of the technology herald FLACS as a revolution that promises superior outcomes and an improved safety profile for patients. Conversely, detractors point to the large financial costs involved and claim that similar results are achievable with conventional small-incision phacoemulsification. This review provides a balanced and comprehensive account of the development of FLACS since its inception. It explains the physiology and mechanics underlying the technology, and critically reviews the outcomes and implications of initial studies. The benefits and limitations of using femtosecond laser accuracy to create corneal incisions, anterior capsulotomy, and lens fragmentation are explored, with reference to the main platforms, which currently offer FLACS. Economic considerations are discussed, in addition to the practicalities associated with the implementation of FLACS in a healthcare setting. The influence on surgical training and skills is considered and possible future applications of the technology introduced. While in its infancy, FLACS sets out the exciting possibility of a new level of precision in cataract surgery. However, further work in the form of large scale, phase 3 randomised controlled trials are required to demonstrate whether its theoretical benefits are significant in practice and worthy of the necessary huge financial investment and system overhaul. Whether it gains widespread acceptance is likely to be influenced by a complex interplay of scientific and socio-economic factors in years to come.

show abstract

Section: Lens Liquefaction and Fragmentationmentioning

confidence: 99%

The journey to femtosecond laser-assisted cataract surgery: new beginnings or a false dawn?

Trikha

Turnbull

Morris

et al. 2013

Eye

View full text Add to dashboard Cite

show abstract

“…Reports of corneal endothelial damage secondary to the use of ultrasound during phacoemulsification have been attributed to the release of free radicals due to cavitation. [131][132][133][134] However, the aqueous humor is rich in antioxidants, including ascorbic acid, and the lens has a coating of glutathione, another effective antioxidant. These endogenous antioxidants provide some endothelial protection.…”

mentioning

confidence: 99%

Potential Adverse Ultrasound-related Biological Effects

2011

View full text Add to dashboard Cite

Ultrasound energy exerts important cellular, genetic, thermal, and mechanical effects. Concern about the safety of ultrasound prompted several agencies to devise regulatory limits on the machine output intensities. The visual display of thermal and mechanical indices during ultrasound imaging provides an aid to limit the output of the machine. Despite many animal studies, no human investigations conducted to date have documented major physiologic consequences of ultrasound exposed during imaging. To date, ultrasound imaging appears to be safe for use in regional anesthesia and pain medicine interventions, and adherence to limiting the output of ultrasound machines as outlined by the Food and Drug Administration may avoid complications in the future. This article reviews ultrasound-related biologic effects, the role of the regulatory agencies in ensuring safety with the use of ultrasound, and the limitations and implications of ultrasound use in humans. P IERRE Curie's discovery of the piezoelectric effect in 1880 launched the ultrasound technology revolution. This technology was first applied in ships for depth detection and in metallurgy for fracture identification, but medical applications were soon appreciated shortly thereafter.1 Medical ultrasound imaging has been used extensively for more than five decades, and the variety of uses for which this technology is used expanded rapidly. For example, the use of ultrasound for interventions during regional anesthesia and pain medicine allows the practitioner to reliably see the target, needle, and injectate with good resolution. 2 The primary advantages of ultrasound in these settings include real-time assessment, absence of radiation, decreased cost, and portability.2

show abstract

“…A szabadgyök-képződés az egyik legfontosabb, a szür-kehályog-műtét során kialakuló endothelsejt-károsodás-hoz vezető folyamat [11][12][13][14][15].…”

Section: Eredeti Közleményunclassified

“…Murano és munkatársainak vizsgálatai szerint az oxidatív stressz apoptózist indukálhat a cornealis endothelsejtekben. A szabad gyökök károsítják a plazmamembránok, a mitokondriumok, az endoplazmatikus reticulum és a sejtmagok membránrendszerét, illetve közvetlenül hatnak a DNS-transzkripcióra, amely hatások következtében sejtoedema és -necrosis alakulhat ki [11].…”

Section: Megbeszélésunclassified

Szabadgyök-felszabadulás vizsgálata femtoszekundum lézerrel asszisztált capsulotomiát követően

et al. 2016

View full text Add to dashboard Cite

Bevezetés: A femtoszekundum lézer alkalmazása forradalmi, innovatív kezelési eljárás a szürkehályog-sebészetben. Célkitűzés: Tanulmányunk célja a femtoszekundum lézeres capsulotomia során az elülső csarnokban képződő szabad gyök mennyiségének meghatározása sertésszemben. Módszer: Hetven friss sertésszemet vontunk be a vizsgálatba, amelyeket post mortem 2 órán belül 4 ºC hőmérsékleten szállítottunk, a kezelést pedig 7 órán belül végeztük el. Harmincöt szemet vizsgáltunk a kontroll-és a femtoszekundum lézeres capsulotomia csoportban is. Luminoldependens kemilumineszcens módszer segítségével vizsgáltuk a csarnokvíz szabadgyök-fogó kapacitását, mint a szabadgyök-termelődés indikátorát. Az emittált fotonok mennyiségét relatív fényegység százalékban fejeztük ki. Eredmé-nyek: A relatív fényegység százalék alacsonyabb volt a kontrollcsoportban (medián 1%, interkvartilis tartomány 0,4-3%), mint a femtoszekundum lézeres capsulotomia csoportban (medián 4,4%, interkvartilis tartomány 1,5-21%) (p = 0,01). Következtetések: A femtoszekundum lézeres capsulotomia gyengíti a csarnokvíz antioxidáns védelmét, amely a femtoszekundum lézeres capsulotomia során felszabaduló szabad gyökök hatására utal. Orv. Hetil., 2016, 157(47), 1880-1883.Kulcsszavak: kemilumineszcencia, kemilumineszcens femtoszekundum lézerrel asszisztált szürkehályog-műtét, szabad gyök, szabadgyök-fogó kapacitás Evaluation of free radical quantity in the anterior chamber following femtosecond laser-assisted capsulotomyIntroduction: Femtosecond laser is a revolutionary, innovative treatment method used in cataract surgery. Aim: To evaluate free radical quantity in the anterior chamber of the eye, during femtosecond laser assisted capsulotomy, in a porcine eye model. Method: Seventy fresh porcine eyes were collected within 2 hours post mortem, were transported at 4 ºC and treated within 7 hours. Thirty-five eyes were used as control and 35 as femtosecond laser assisted capsulotomy group. A simple luminol-dependent chemiluminescence method was used to measure the total scavenger capacity in the aqueous humour, as an indicator of free radical production. The emitted photons were expressed in relative light unit %. Results: The relative light unit % was lower in the control group (median 1%, interquartile range [0.4-3%]) than in the femtosecond laser assisted capsulotomy group (median 4.4%, interquartile range [1.5%-21%]) (p = 0.01). Conclusions: Femtosecond laser assisted capsulotomy decreases the antioxidant defense of the anterior chamber, which refers to a significant free radical production during femtosecond laser assisted capsulotomy.

show abstract

Corneal Endothelial Cell Damage by Free Radicals Associated With Ultrasound Oscillation

Cited by 77 publications

References 31 publications

The journey to femtosecond laser-assisted cataract surgery: new beginnings or a false dawn?

The journey to femtosecond laser-assisted cataract surgery: new beginnings or a false dawn?

Potential Adverse Ultrasound-related Biological Effects

Szabadgyök-felszabadulás vizsgálata femtoszekundum lézerrel asszisztált capsulotomiát követően

Contact Info

Product

Resources

About