Core/shell fluorescent magnetic silica-coated composite nanoparticles for bioconjugation

He, Rong; You, Xiaogang; Shao, Jun; Gao, Feng; Pan, Bifeng; Cui, Daxiang

doi:10.1088/0957-4484/18/31/315601

Cited by 109 publications

(84 citation statements)

References 24 publications

(43 reference statements)

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“…14,41 In this work we have described the synthesis of well-defined core/shell nanoparticles comprising a core of lanthanopolyoxotunsgstates encapsulated by silica shells, which in principle can be further exploited as alternative bio-labels. The visible photoluminescence of the POM/SiO 2 systems can be tuned by modifying the coordination sphere of the lanthanopolyoxometalate and/or its chemical composition.…”

Section: Discussionmentioning

confidence: 99%

Lanthanopolyoxotungstates in silica nanoparticles: multi-wavelength photoluminescent core/shell materials

et al. 2010

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Photoluminescent lanthanopolyoxotungstate core/shell nanoparticles are prepared by the encapsulation of lanthanide-containing polyoxometalates (POMs) with amorphous silica shells. The preparation of morphological well-defined core/shell nanoparticles is achieved by the hydrolysis of tetraethoxysilane in the presence of POMs using a reverse microemulsion method. The POMs used are decatungstolanthanoates of [Ln(W 5 9À type (Ln(III) ¼ Eu, Gd and Tb). Photoluminescence studies show that there is efficient emission from the POM located inside the SiO 2 shells, through excitation paths that involve O / Eu/Tb and O / W ligand-to-metal charge transfer. It is also shown that the excitation of the POM containing europium(III) may be tuned towards longer wavelengths via an antenna effect, by coordination of an organic ligand such as 3-hydroxypicolinate. The POM/SiO 2 nanoparticles form stable suspensions in aqueous solution having the advantage of POM stabilization inside the core and the possibility of further surface grafting of chemical moieties via well known derivatization procedures for silica surfaces. These features together with the possibility of tuning the excitation wavelength by modifying the coordination sphere in the lanthanopolyoxometalate, make this strategy promising to develop a new class of optical bio-tags composed of silica nanobeads with multi-wavelength photoluminescent lanthanopolyoxometalate cores.

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Section: Discussionmentioning

confidence: 99%

Lanthanopolyoxotungstates in silica nanoparticles: multi-wavelength photoluminescent core/shell materials

et al. 2010

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“…Other studies have shown that the magnetic core can be replaced by semiconductor quantum dots or fluorescence molecules doped into the silica shell to form fluorescent core/shell silica nanoparticles. [9][10][11] These silica-coated fluorescent magnetic nanoprobes enabled development of biomedical platforms for simultaneous imaging, diagnosis, and therapy. [12][13][14] However, it is still a challenge to fabricate fluorescent magnetic nanoprobes with high photostability, high payloads of dye, and desirable outer surfaces for further modification with functional or target moieties.…”

Section: Introductionmentioning

confidence: 99%

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Yang¹,

Zhao²,

Liu³

et al. 2013

IJN

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Multifunctional nanomaterials with unique magnetic and luminescent properties have broad potential in biological applications. Because of the overexpression of vascular cell adhesion molecule-1 (VCAM-1) receptors in inflammatory endothelial cells as compared with normal endothelial cells, an anti-VCAM-1 monoclonal antibody can be used as a targeting ligand. Herein we describe the development of multifunctional core-shell Fe 3 O 4 @SiO 2 nanoparticles with the ability to target inflammatory endothelial cells via VCAM-1, magnetism, and fluorescence imaging, with efficient magnetic resonance imaging contrast characteristics. Superparamagnetic iron oxide and fluorescein isothiocyanate (FITC) were loaded successfully inside the nanoparticle core and the silica shell, respectively, creating VCAM-1-targeted Fe 3 O 4 @ SiO 2 (FITC) nanoparticles that were characterized by scanning electron microscopy, transmission electron microscopy, fluorescence spectrometry, zeta potential assay, and fluorescence microscopy. The VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles typically had a diameter of 355 ± 37 nm, showed superparamagnetic behavior at room temperature, and cumulative and targeted adhesion to an inflammatory subline of human umbilical vein endothelial cells (HUVEC-CS) activated by lipopolysaccharide. Further, our data show that adhesion of VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles to inflammatory HUVEC-CS depended on both shear stress and duration of exposure to stress. Analysis of internalization into HUVEC-CS showed that the efficiency of delivery of VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles was also significantly greater than that of nontargeted Fe 3 O 4 @SiO 2 (FITC)-NH 2 nanoparticles. Magnetic resonance images showed that the superparamagnetic iron oxide cores of the VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles could also act as a contrast agent for magnetic resonance imaging. Taken together, the cumulative adhesion and uptake potential of these VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles targeted to inflammatory endothelial cells could be used in the transfer of therapeutic drugs/genes into these cells or for diagnosis of vascular disease at the molecular and cellular levels in the future.

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“…The preparation and characterization of FMNPs were reported in our previous papers. [25][26][27] The iPS cells were seeded on the cover slips and treated with growth medium containing FMNPs (50 µg/mL) for 4 hours at 37°C with 5% CO 2 . Afterwards, the cells were rinsed with PBS and fixed with 2.5% glutaraldehyde for 30 minutes.…”

Section: Labeling Ips Cells With Fmnpsmentioning

confidence: 99%

Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology

Ruan¹,

Shen²,

Wang³

et al. 2011

IJN

Self Cite

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Efficient preparation and labeling of human induced pluripotent stem (iPS) cells is a great challenge in stem cell research and development. With the aim of investigating the feasibility of using nanotechnology to enhance the preparation efficiency of iPS cells and to label iPS cells for long-term tracing and imaging, in this paper, four transcription factor genes, ie, Oct4, Sox2, LIN28, and Nanog, and packaging plasmids such as PSPAX2 and PMD2.G were cotransfected into 293T cells using Generation 5.0 polyamidoamine dendrimer-modified magnetic nanoparticles (dMNPs) as a delivery system. The resultant supernatant liquids were incubated with human fibroblast cells at 37°C for 21 days, then the embryonic stem (ES) cell-like clones were screened, cultured, and identified. Finally, the prepared iPS cells were labeled with fluorescent magnetic nanoparticles (FMNPs). The results showed that dMNPs can efficiently deliver all vectors into 293T cells. The resultant lentiviruses’ titers were 10-fold more than those based on Lipofectamine™ 2000. Reverse transcription polymerase chain reaction analysis showed that four genes (Oct4, Sox2, LIN28, and Nanog) exhibited different expressions in iPS cells. Immunostaining analysis showed that specific surface markers of ES cells such as SSEA-3, SSEA-4, Tra-1-60, and Tra-1-81 were positive in iPS cells, and the terotomas were formed in NOD-SCID mice that were implanted with iPS cells. Red fluorescent signals could be observed in iPS cells labeled with FMNPs by fluorescent microscopy, and the magnetic signals were detected in labeled iPS cells by magnetic resonance imaging. In conclusion, human iPS cells can be efficiently generated using polyamidoamine dMNPs and lentivirus and labeled with FMNPs for long-term observation and tracking, which has great potential application in the research and development of stem cells in the near future.

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Core/shell fluorescent magnetic silica-coated composite nanoparticles for bioconjugation

Cited by 109 publications

References 24 publications

Lanthanopolyoxotungstates in silica nanoparticles: multi-wavelength photoluminescent core/shell materials

Lanthanopolyoxotungstates in silica nanoparticles: multi-wavelength photoluminescent core/shell materials

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology

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