Copper Nanoparticles as Modulators of Apoptosis and Structural Changes in Tissues

Sizova, Еlena; Miroshnikov, Sergey; Polyakova, Valentina S.; Gluschenko, N. N.; Skalny, Anatoly V.

doi:10.4236/jbnb.2012.31013

Cited by 20 publications

(9 citation statements)

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“…This, however, may be unrelated to the capture by Kupffer cells of any NPs from the blood, since it is well known that resident macrophages play multiple roles in the process of apoptosis of an organ’s functional cells, engulfing, in particular, the apoptotic bodies (e.g., [ 31 ]), and that in the liver just the Kupffer cells play this role [ 32 ]. Thus, Kupffer cell activation may serve as indirect indicator of copper’s cytotoxic effect, which is known to be associated with apoptosis, at least, for copper NPs [ 33 ]. The lack of this effect of NPs against the background of bio-protectors reducing copper toxicity as seen in the same chart ( Figure 3 ) provides evidence in favor of such interpretation.…”

Section: Resultsmentioning

confidence: 99%

Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors

Privalova

Katsnelson

Loginova

et al. 2014

IJMS

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In the copper metallurgy workplace air is polluted with condensation aerosols, which a significant fraction of is presented by copper oxide particles <100 nm. In the scientific literature, there is a lack of their in vivo toxicity characterization and virtually no attempts of enhancing organism’s resistance to their impact. A stable suspension of copper oxide particles with mean (±SD) diameter 20 ± 10 nm was prepared by laser ablation of pure copper in water. It was being injected intraperitoneally to rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week up to 19 injections. In parallel, another group of rats was so injected with the same suspension against the background of oral administration of a “bio-protective complex” (BPC) comprising pectin, a multivitamin-multimineral preparation, some amino acids and fish oil rich in ω-3 PUFA. After the termination of injections, many functional and biochemical indices for the organism’s status, as well as pathological changes of liver, spleen, kidneys, and brain microscopic structure were evaluated for signs of toxicity. In the same organs we have measured accumulation of copper while their cells were used for performing the Random Amplification of Polymorphic DNA (RAPD) test for DNA fragmentation. The same features were assessed in control rats infected intraperitoneally with water with or without administration of the BPC. The copper oxide nanoparticles proved adversely bio-active in all respects considered in this study, their active in vivo solubilization in biological fluids playing presumably an important role in both toxicokinetics and toxicodynamics. The BPC proposed and tested by us attenuated systemic and target organs toxicity, as well as genotoxicity of this substance. Judging by experimental data obtained in this investigation, occupational exposures to nano-scale copper oxide particles can present a significant health risk while the further search for its management with the help of innocuous bioprotectors seems to be justified.

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Section: Resultsmentioning

confidence: 99%

Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors

Privalova

Katsnelson

Loginova

et al. 2014

IJMS

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“…Effluent, spillage during shipping, and handling are considered as the main routes of entry for CNPs to human body [ 2 ]. Regarding their small size and high reactivity, various studies showed that CNPs could causes a diversity of toxic effects including hepatotoxicity [ 3 , 4 ]. Chen et al [ 4 ] reported that CNPs’ toxicity is triggered by reactive oxygen species (ROS) over production.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Influence of Green Tea Extract on Copper Nanoparticle-Induced Hepatotoxicity in Rats

Ibrahim¹,

Khalaf²,

Galal³

et al. 2015

Nanoscale Res Lett

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The potential toxicity of copper nanoparticles (CNPs) to the human health and environment remains a critical issue. In the present study, we investigated the protective influence of an aqueous extract of green tea leaves (GTE) against CNPs-induced (20–30 nm) hepatotoxicity. Four different groups of rats were used: group I was the control, group II received CNPs (40 mg/kg BW), group III received CNPs plus GTE, and group IV received GTE alone. We highlighted the hepatoprotective effect of GTE against CNPs toxicity through monitoring the alteration of liver enzyme activity, antioxidant defense mechanism, histopathological alterations, and DNA damage evaluation. The rats that were given CNPs only had a highly significant elevation in liver enzymes, alteration in oxidant-antioxidant balance, and severe pathological changes. In addition, we detected a significant elevation of DNA fragmentation percentage, marked DNA laddering, and significance over expression of both caspase-3 and Bax proteins. The findings for group III clarify the efficacy of GTE as a hepatoprotectant on CNPs through improving the liver enzyme activity, antioxidant status, as well as suppressing DNA fragmentation and the expression of the caspase-3 and Bax proteins. In conclusion, GTE was proved to be a potential hepatoprotective additive as it significantly ameliorates the hepatotoxicity and apoptosis induced by CNPs.

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“…Effluent, spillage during shipping, and handling are considered as the main routes of entry for CNPs to human body [2]. Regarding their small size and high reactivity, various studies showed that CNPs could causes a diversity of toxic effects including hepatotoxicity [3,4]. Chen et al [4] reported that CNPs' toxicity is triggered by reactive oxygen species (ROS) over production.…”

mentioning

confidence: 99%

Ameliorative Influence of Green Tea Extract on Copper Nanoparticle-Induced Hepatotoxicity in Rats

Ibrahim¹,

Khalaf²,

Galal³

et al. 2016

Nano Online

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(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The potential toxicity of copper nanoparticles (CNPs) to the human health and environment remains a critical issue. In the present study, we investigated the protective influence of an aqueous extract of green tea leaves (GTE) against CNPs-induced (20-30 nm) hepatotoxicity. Four different groups of rats were used: group I was the control, group II received CNPs (40 mg/kg BW), group III received CNPs plus GTE, and group IV received GTE alone. We highlighted the hepatoprotective effect of GTE against CNPs toxicity through monitoring the alteration of liver enzyme activity, antioxidant defense mechanism, histopathological alterations, and DNA damage evaluation.The rats that were given CNPs only had a highly significant elevation in liver enzymes, alteration in oxidant-antioxidant balance, and severe pathological changes. In addition, we detected a significant elevation of DNA fragmentation percentage, marked DNA laddering, and significance over expression of both caspase-3 and Bax proteins. The findings for group III clarify the efficacy of GTE as a hepatoprotectant on CNPs through improving the liver enzyme activity, antioxidant status, as well as suppressing DNA fragmentation and the expression of the caspase-3 and Bax proteins. In conclusion, GTE was proved to be a potential hepatoprotective additive as it significantly ameliorates the hepatotoxicity and apoptosis induced by CNPs.Keywords: CNPs; Liver; Apoptosis; Oxidative stress; Green tea extract; Rats BackgroundAs more and more nanomaterials are introduced in our daily life, serious environmental hazard could occur. Copper nanoparticles (CNPs) are one of the first engineered nanoparticles (NPs) involved in a variety of industrial applications, such as facial spray, lubricants additive, metallic coating, and inks [1]. Effluent, spillage during shipping, and handling are considered as the main routes of entry for CNPs to human body [2]. Regarding their small size and high reactivity, various studies showed that CNPs could causes a diversity of toxic effects including hepatotoxicity [3,4]. Chen et al. [4] reported that CNPs' toxicity is triggered by reactive oxygen species (ROS) over production. Usually, cells respond to oxidative burden through fortifying their antioxidant defense mechanism. However, the imbalance between oxidative burden and defense mechanism induces protein oxidation, lipid peroxidation (LPO), DNA damage, and apoptosis [5,6]. Recently, much interest had been focused on the role of naturally occurred herbal plant extracts as protective agents for various toxins [7]. Green tea extract (GTE) had attracted a great attention for its health benefits against a variety of toxins associated with oxidative stress [7,8]. Many studies proved the hepatoprotective role of GT...

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Copper Nanoparticles as Modulators of Apoptosis and Structural Changes in Tissues

Cited by 20 publications

References 11 publications

Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors

Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors

Ameliorative Influence of Green Tea Extract on Copper Nanoparticle-Induced Hepatotoxicity in Rats

Ameliorative Influence of Green Tea Extract on Copper Nanoparticle-Induced Hepatotoxicity in Rats

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