Copper nanoparticles and copper ions promote horizontal transfer of plasmid-mediated multi-antibiotic resistance genes across bacterial genera

Zhang, Shuai; Wang, Yue; Song, Hai-Liang; Lu, Ji; Yuan, Zhiguo; Guo, Jianhua

doi:10.1016/j.envint.2019.05.054

Cited by 216 publications

(139 citation statements)

References 41 publications

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“…The heavy metal NPs/ions-promoted natural transformation was found to be associated with ROS overproduction, as validated by the reversal of the transformation frequency to baseline levels with the addition of a ROS scavenger-thiourea ( Figure 2G), with ZnO NPs as an exception. The observed transformation promoted by heavy metal-based NPs/ions was in agreement with our previous findings that CuO and Ag NPs/ions could enhance conjugative ARGs transfer from Escherichia coli to Pseudomonas putida via over-generation of ROS (18,19) Likewise, recent studies have also indicated that solar disinfection and water disinfection by-products could increase the natural transformation rates of extracellular DNA in A. baylyi via inducing ROS (24,25).…”

Section: Heavy Metal Nanoparticles and Ions Promoted Args Transformatsupporting

confidence: 91%

“…To evaluate the effects of Ag, CuO and ZnO NPs/ions on cell membrane morphology, A. baylyi cells after 2 h treatment with Ag NPs/ions (1 mg/L), CuO NPs/ions (1 mg/L) and ZnO NPs/ions (10 mg/L) and one untreated A. baylyi were observed under a JEM-1010 Transmission Electron Microscope (JEOL, USA) at 80 kV according to the method previously described [18].…”

Section: Transmission Electron Microscope Imaging Of Nps/ions-treatedmentioning

confidence: 99%

“…For instance, heavy metal ions (e.g., Cu 2+ , Cd 2+ , Hg 2+ and Zn 2+ ) and nanoparticles (e.g., ZnO NPs, Al 2 O 3 NPs and Ag NPs) have been reported to induce antibiotic resistance via co-selection or direct mutation [12,13,15,16]. In addition, heavy metal ions (including Cu 2+ , Ag + , Cr 6+ , Ti 4+ and Zn 2+ ) [17][18][19] and nanoparticles (e.g., Al 2 O 3 NPs, CuO NPs, Ag NPs and TiO 2 NPs) [18][19][20][21] could promote the transfer of ARGs via conjugation. However, it is unclear whether heavy metal-based NPs and ions could enhance the dissemination of ARGs via natural transformation.…”

Section: Introductionmentioning

confidence: 99%

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Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Guo

Zhang

et al. 2020

Preprint

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Background: Antibiotic resistance genes (ARGs), heavy metal ions and nanoparticles (NPs) are emerging and ubiquitous contaminants in the environment. However, little is known about whether heavy metal-based NPs or ions could facilitate the dissemination of ARGs through natural transformation. This study evaluated the contributions of heavy metal-based NPs (Ag NPs, CuO NPs and ZnO NPs) and their ion forms (Ag + , Cu 2+ and Zn 2+ ) to the transformation of extracellular ARGs in Acinetobacter baylyi ADP1. Results: We found that these commonly-used NPs and ions from environmentally relevant concentrations can significantly promote the natural transformation frequency of ARGs by a factor of 11.0-folds, which is comparable to the effects of antibiotics. The enhanced transformation by Ag NPs, CuO NPs, Ag + and Cu 2+ was primarily associated with reactive oxygen species (ROS) over-production and cell membrane damage, which was also evident from up-regulations of both transcription and translation of ROS and outer membrane-related genes. Additionally, transmission electron microscope imaging revealed the roughened cell membrane after Ag NPs, CuO NPs, Ag + and Cu 2+ exposure. ZnO NPs and Zn 2+ might increase the natural transformation rate by stimulating the stress response and ATP synthesis. All tested NPs and ions resulted in up-regulating the competence and SOS response-associated genes. Conclusions: Our results demonstrate that Ag, CuO and ZnO-based NPs/ions from environmental concentrations could promote the natural transformation of plasmid-encoded ARGs into naturally competent A. baylyi . Our findings provide insights into the contributions of heavy metals and NPs to the spread of antibiotic resistance.

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Section: Heavy Metal Nanoparticles and Ions Promoted Args Transformatsupporting

confidence: 91%

Section: Transmission Electron Microscope Imaging Of Nps/ions-treatedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Guo

Zhang

et al. 2020

Preprint

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“…This phenomenon has been confirmed in recent years. Zhang reported that copper nanoparticles and copper ions promote plasmid‐mediated horizontal transfer of multiple antibiotic resistance genes in certain bacteria. Excessive production of ROS also plays a key role in promoting conjugate transfer.…”

Section: Limitations and Prospectsmentioning

confidence: 99%

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

Wang

Yuan

et al. 2019

Adv Healthcare Materials

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Due to their excellent size, designability, and outstanding targeted antibacterial effects, nanoparticles have become a potential option for controlling oral biofilm‐related infections. However, the formation of an oral biofilm is a dynamic process, and factors affecting the performance of antibiofilm treatments are complex. As such, when examining the existing literature on the antibiofilm effects of nanoparticles, attention should be paid to the specific mechanisms of action at different stages of oral biofilm formation, as well as relevant influencing factors, in order to achieve an objective and comprehensive evaluation. This review is intended to detail the antibacterial mechanisms of nanoparticles during the four stages of the formation of oral biofilms: 1) acquired film formation; 2) bacterial adhesion; 3) early biofilm development; and 4) biofilm maturation. In addition, factors influencing the antibiofilm properties of nanoparticles are summarized from the aspects of nanoparticles themselves, biofilm models, and host factors. The limitations of current research and possible trends for future research are also discussed. In summary, nanoparticles are a promising antioral biofilm strategy. It is hoped that this review can serve as a reference and inspire ideas for further research on the application of nanoparticles for effectively targeting and treating oral biofilms.

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“…Horizontal gene transfer (HGT) has regarded as the major pathway of ARGs transmission, which includes conjugation, transformation and transduction [9]. As a dominant pathway of HGT, conjugation is the transfer of ARGs through a pilin bridge between a donor and a recipient, which is usually achieved via mobile genetic elements (MGEs), such as plasmids (pMV158oriT), insertion sequences (IS613), integrons (intI-1), transposons (tnpA-03), and phages [10,11]. HGT can occur in any environment, especially where bacterial densities are high, such as in soil [12], in the gut microbiome of humans and animals [4]; and it has been shown to provide microbes with a wide variety of adaptive traits for microbial survival and proliferation under the pressure of antibiotics, heavy metals and other environmental contaminants [13].…”

Section: Introductionmentioning

confidence: 99%

Horizontal Transfer Mediated Proliferation of Antibiotic Resistance Genes in High-Fat Diet Induced Obesity Mice

Wang¹,

Chen²,

Wang³

et al. 2020

Preprint

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Background Infections caused by pathogens carrying antibiotic resistance genes (ARGs) are triggering a global health crisis. Human gut microbiota is an important reservoir of ARGs, which can not only be exchanged inside the symbiotic microbiota, but can also be horizontally transferred to opportunistic pathogens. A dysbiotic gut microbiota has been associated with obesity and other metabolic disorders, which has also been described as the most unneglected global health problem. Many studies have focused on the effects of antibiotics and other exogenous chemical on the fate of ARGs, but little is known about the effect of obesity induced dysbiotic gut microbiota on the fate of ARGs. Results This study addressed the significant proliferation of ARGs and the dramatical change of mobile genetic elements (MGEs) in high-fat diet induced obesity (DIO) mice, which the ermB and tnpA-03 genes mostly increased, demonstrating that DIO alone could enrich the abundance of ARGs. A clear increase of serine and tyrosine in the feces showed a significant positive correlation with the dominant genes of DIO groups (ermB and tnpA-03), suggesting that serine and tyrosine might enhance DNA transposition (tnpA-03) by nucleophilic attacking a phosphorus atom of a backbone phosphate group and ultimately significantly amplified the ARGs. Additionally, ermB has been frequently found in obesity-associated Lactobacillus, which might contribute to the sharp increase of ARGs and dramatical change of MGEs in the DIO mice. Finally, procrustes analysis showed the explanatory variables of the MGEs, the metabolites, and the microbial communities for the ARGs accounted for 94.3%, 53.4%, and 68.1%, respectively. Conclusion This study demonstrated that DIO significantly enhanced the abundance of ARGs and dramatically changed MGEs in mouse gut microbiota. MGEs were the most direct factor affecting ARGs, and microbiota were the main driver of the proliferation of ARGs in the DIO mice.

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Copper nanoparticles and copper ions promote horizontal transfer of plasmid-mediated multi-antibiotic resistance genes across bacterial genera

Cited by 216 publications

References 41 publications

Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

Horizontal Transfer Mediated Proliferation of Antibiotic Resistance Genes in High-Fat Diet Induced Obesity Mice

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