Copan microFLOQ® Direct Swab collection of bloodstains, saliva, and semen on cotton cloth

Sherier, Allison J; Kieser, Rachel; Novroski, Nicole M.M.; Wendt, Frank R.; King, Jonathan L.; Woerner, August E.; Ambers, Angie; Garofano, Paolo; Budowle, Bruce

doi:10.1007/s00414-019-02081-6

Cited by 13 publications

(17 citation statements)

References 21 publications

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“…Studies have also shown that allele peak heights were consistently higher for diluted blood and saliva samples when compared with PCR amplification using 1 ng of purified DNA (Ambers et al, 2018). Since then, the microFLOQ Direct swab has been applied to a range of biological samples (Ambers et al, 2018; Chong et al, 2019; Sherier et al, 2020).…”

Section: Bypassing Dna Extraction (Direct Pcr Amplification)mentioning

confidence: 99%

“…For example, the Casework Direct kit, Custom (Promega) contains a buffer and reducing agent to generate PCR amplification‐ready lysates from biological stains and touch samples (Graham et al, 2018; Hakim et al, 2019). In one study, the authors used a combination of microFLOQ™ Direct swabs for sample collection together with pre‐PCR amplification treatment using the Casework Direct kit, Custom to prepare lysates for direct PCR amplification (Sherier et al, 2020). Subsequently, Y. Wong et al (2019) showed that low TE buffer could be used as a more economical alternative to the Casework Direct kit, Custom for use with the microFLOQ Direct swab.…”

Section: Bypassing Dna Extraction (Direct Pcr Amplification)mentioning

confidence: 99%

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Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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The extraction of DNA is a fairly standard procedure routinely undertaken in biological academic and research settings. In a forensic DNA context, DNA extraction is the crucial first step toward the generation of DNA profiles used to support convictions or exonerations in the criminal justice system. However, it is often complicated by the additional challenge of yielding sufficient quantities of clean DNA that is free from environmental inhibitors, from a wide spectrum of sample types which may contain limited quantities of biological material. While improvements in methods and technology over the decades have sped up and simplified the process of DNA extraction, several issues remain to be fully addressed—such as the incomplete separation of spermic DNA from sperm‐epithelial cell mixtures, increasing demands for ever‐faster DNA results, or even on‐scene DNA processing, and processing of difficult samples such as skeletal remains or samples contaminated by Chemical, Biological, or Radiological (CBR) agents. These challenges are often compounded by high work volumes and limited quantities of starting material. As such, this review focuses on recent trends and developments in four areas of forensic DNA extraction: (a) analysis of sexual assault evidence which often represents a significant portion of a forensic DNA laboratory's casework, (b) use of portable rapid DNA instruments which has gained much traction among law enforcement in recent times, (c) DNA extraction from difficult and CBR‐contaminated samples which, albeit rare, would be essential in a DNA laboratory's toolbox in the event of an incident, and (d) bypassing DNA extraction altogether. This article is categorized under: Forensic Biology > Forensic DNA Technologies

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Section: Bypassing Dna Extraction (Direct Pcr Amplification)mentioning

confidence: 99%

Section: Bypassing Dna Extraction (Direct Pcr Amplification)mentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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“…A further study indicated that the use of pieces of absorbing paper moistened with 70% ethanol recovered epithelial cells/touch DNA from various surface types better than a cotton swab moistened with water [82]. In addition, Sherier et al [83] illustrated the potential benefits of using the microFLOQ ® Direct Swab (Copan, Brescia, Italy) for collecting DNA from minute stains, and possibly using it as a pre-screening methodology (i.e., sampling a small portion of a stain for initial screening and testing for expeditious processing) while saving the remainder of the stain for later testing. They also demonstrated that swabbing the centre of stains of blood, semen and saliva provided more DNA and complete profiles than swabbing the edge of stains.…”

Section: Samplingmentioning

confidence: 99%

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Oorschot

Meakin

Kokshoorn

et al. 2021

Genes

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Understanding the factors that may impact the transfer, persistence, prevalence and recovery of DNA (DNA-TPPR), and the availability of data to assign probabilities to DNA quantities and profile types being obtained given particular scenarios and circumstances, is paramount when performing, and giving guidance on, evaluations of DNA findings given activity level propositions (activity level evaluations). In late 2018 and early 2019, three major reviews were published on aspects of DNA-TPPR, with each advocating the need for further research and other actions to support the conduct of DNA-related activity level evaluations. Here, we look at how challenges are being met, primarily by providing a synopsis of DNA-TPPR-related articles published since the conduct of these reviews and briefly exploring some of the actions taken by industry stakeholders towards addressing identified gaps. Much has been carried out in recent years, and efforts continue, to meet the challenges to continually improve the capacity of forensic experts to provide the guidance sought by the judiciary with respect to the transfer of DNA.

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“…In analytical chemistry and forensic toxicology when these measurements are matched the analyst can conclude that the endogenous peptide is in the sample [74,75]. Using pre-defined retention time windows and precursor and fragment mass information, these targeted assays may be developed to simultaneously and reproducibly detect hundreds of peptides in a single run, with high sensitivity, precision and accuracy [50,70,76,77]. At a practical level QQQ mass spectrometers have lower running costs, daily maintenance is more straightforward, and they are easier to use [76,78,79].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%