Copaifera langsdorffii: evaluation of potential gastroprotective of extract and isolated compounds obtained from leaves

Abstract

Revista Brasileira de Farmacognosia 25 (2015) 238-245 w w w . s b f g n o s i a . o r g . b r / r e v i s t a a b s t r a c tGastric ulcer is a prevalent gastrointestinal disease, and the drugs currently used in the treatment produce several adverse effects. In this context, the search for new therapeutic antiulcer agents is essential, and medicinal plants have great potential. Here, we investigated the gastroprotective properties of Copaifera langsdorffii Desf., Fabaceae, hydroalcoholic extract obtained from leaves and its isolated compounds. The phytochemistry studies and the compounds isolations were performed using chromatographic and spectroscopic methodologies. The hydroalcoholic extract was evaluated using ethanol/HCl, non-steroidal anti-inflammatory drug, stress-induced-ulcer and chronic ulcer-model. The effects on gastric content volume, pH, total acidity and mucus stomach production were evaluated in the pylorus ligated-model. The C. langsdorffii extract obtained from leaves (50, 250 or 500 mg/kg) reduced the injured area compared to control group in all experiments. The extract showed a significant decrease in the total gastric juice acidity and an increase in mucus production (500 mg/kg) when compared to vehicle. Among isolated compounds (30 mg/kg) ␣-humulene, ␤-caryophyllene and caryophyllene oxide showed greater gastroprotective activity in the ethanol/HCl induced ulcer model. The data herein obtained shown that C. langsdorffii leaves extract and isolated compounds from it, presented gastroprotective properties in different animal models of gastric ulcer. These effects may be associated with the ability of the extract to decrease gastric secretion and increase the mucus production.

Quantitative analysis of phenolic metabolites in Copaifera langsdorffii leaves from plants of different geographic origins cultivated under the same environmental conditions

“…The leaves of C. langsdorffii have been chemically and pharmacologically studied due to their medicinal properties, including gastroprotection and kidney stones prevention. [13][14][15][16] In order to propose criteria for the standardisation of medicinal plants, it is essential to know the chemical variability of the plant species used as raw material. We conducted a seasonal study of metabolites in leaves of C.…”

“…The same extract also prevented kidney stone recurrence . Moreover, gastroprotection activity was found for both leaf extract and the main secondary metabolites found in C. langsdorffii leaves …”

“…The main chemical constituents of C. langsdorffii leaves include galloylquinic acid (GQA) derivatives and flavonoids, which are associated with this plant's pharmacological activities . To facilitate the development of a new phytomedicine using C. langsdorffii leaves as raw material, this study examines the influence of environmental and seasonal factors on the production of phenolic metabolites in C. langsdorffii leaves.…”

“…15,16 The main chemical constituents of C. langsdorffii leaves include galloylquinic acid (GQA) derivatives 16,17 and flavonoids, 17,18 which are associated with this plant's pharmacological activities. [13][14][15][16] To facilitate the development of a new phytomedicine using C. langsdorffii leaves as raw material, this study examines the influence of environmental and seasonal factors on the production of phenolic metabolites in C. langsdorffii leaves. For that, copaiba plants obtained from seeds collected in 10 different regions of two main states in Brazil were cultivated in a single field and exposed to the same environmental conditions.…”

Estudo químico e desenvolvimento de métodos analíticos validados em cromatografia para análise de oleorresinas e extratos de folhas de espécies de Copaifera

“…As plantas conhecidas popularmente como: copaíba, copaibeiras, copaíferas, pau , 2008;LEANDRO et al, 2012;LEMOS, et al, 2015). As árvores desse gênero apresentam grande importância social, econômica e medicinal em virtude da produção de um exsudato extraído do tronco sendo constituído de uma mistura de ácidos resinosos e substâncias voláteis (mistura de diterpenos ácidos e sesquiterpenos, respectivamente), denominado de oleorresina, que possui composição variável em função de fatores bióticos e abióticos (PLOWDEN, 2003;LEANDRO et al, 2012).…”