COP1 is required for UV-B–induced nuclear accumulation of the UVR8 photoreceptor

Yin, Ruohe; Skvortsova, Mariya; Loubéry, Sylvain; Ulm, Roman

doi:10.1073/pnas.1607074113

Cited by 127 publications

(123 citation statements)

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“…In Arabidopsis, the COP1-HY5 interaction has been well established (e.g. Ang et al, 1998); the interaction between COP1 and HY5 is UVB dependent: there is no interaction in the absence of photomorphogenic UVB (Favory et al, 2009;Yin et al, 2016). Therefore, our Y2H experimental data among CsUVR8, CsCOP1, and CsHY5 were consistent with findings in Arabidopsis, suggesting that cucumber may take a similar UVR8 signaling pathway for UVB stress responses.…”

Section: Yeast Two-hybrid Growth Assay Reveals Interaction Between Shsupporting

confidence: 84%

“…In Arabidopsis, it is well established that, under UVB, interaction of UVR8 with COP1 stabilizes HY5 (Favory et al, 2009;Huang et al, 2012;Cloix et al, 2012;Yin et al, 2015Yin et al, , 2016 and enhances the association of HY5 with target promoters, including its own (Oravecz et al, 2006;Abbas et al, 2014;Binkert et al, 2014). HY5 then activates transcription of many UVR8-regulated genes, which are associated with hypocotyl elongation inhibition, UVB protection, or acclamation (Brown et al, 2005;Oravecz et al, 2006;Huang et al, 2012;Binkert et al, 2014).…”

Section: Role Of Sh1 In Lduvb-dependent Hypocotyl Elongation: a Modelmentioning

confidence: 99%

“…However, few molecular players involved in UVR8-mediated UVB signal transduction are currently known, including COP1 and HY5 (Ulm et al, 2004;Brown et al, 2005;Oravecz et al, 2006;Favory et al, 2009;Huang et al, 2012;Binkert et al, 2014;Ulm and Jenkins 2015). Under this model, the monomerized active UVR8 interacts with COP1, leading to the expression and stabilization of HY5, which directly bind to the promoter of several UVB responsive genes (Rizzini et al, 2011;Cloix et al, 2012;Binkert et al, 2014;Jenkins, 2014;Yin et al, 2015Yin et al, , 2016.…”

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confidence: 99%

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SHORT HYPOCOTYL 1 Encodes a SMARCA3-like Chromatin Remodeling Factor Regulating Elongation

Bo¹,

Wang²,

Pan³

et al. 2016

Plant Physiol.

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(Y.We.).In Arabidopsis (Arabidopsis thaliana), the UVR8-mediated signaling pathway is employed to attain UVB protection and acclimation to deal with low-dosage UVB (LDUVB)-induced stresses. Here, we identified SHORT HYPOCOTYL1 (SH1) in cucumber (Cucumis sativus), which regulates LDUVB-dependent hypocotyl elongation by modulating the UVR8 signaling pathway. We showed that hypocotyl elongation in cucumbers carrying the recessive sh1 allele was LDUVB insensitive and that Sh1 encoded a human SMARCA3-like chromatin remodeling factor. The allele frequency and distribution pattern at this locus among natural populations supported the wild cucumber origin of sh1 for local adaptation, which was under selection during domestication. The cultivated cucumber carries predominantly the Sh1 allele; the sh1 allele is nearly fixed in the semiwild Xishuangbanna cucumber, and the wild cucumber population is largely at Hardy-Weinberg equilibrium for the two alleles. The SH1 protein sequence was highly conserved among eukaryotic organisms, but its regulation of hypocotyl elongation in cucumber seems to be a novel function. While Sh1 expression was inhibited by LDUVB, its transcript abundance was highly correlated with hypocotyl elongation rate and the expression level of cell-elongationrelated genes. Expression profiling of key regulators in the UVR8 signaling pathway revealed significant differential expression of CsHY5 between two near isogenic lines of Sh1. Sh1 and CsHY5 acted antagonistically at transcriptional level. A working model was proposed in which Sh1 regulates LDUVB-dependent hypocotyl elongation in cucumber through changing the chromatin states and thus the accessibility of CsHY5 in the UVR8 signaling pathway to promoters of LDUVB-responsive genes for hypocotyl elongation.The hypocotyl of dicotyledonous plants is the embryonic stem connecting the two embryonic leaves (cotyledons) and the primary root (radicle). The hypocotyl, which has a relatively simple architecture (Scheres et al., 1994), is a very plastic organ, strongly influenced by both external and internal cues known to regulate cell elongation, such as light, gravity, temperature, and hormones. In Arabidopsis (Arabidopsis thaliana), after germination, hypocotyl growth relies mainly on longitudinal cell elongation rather than cell divisions; its length can increase by more than 10-fold (Gendreau et al., 1997). The morphological simplicity, growth behavior, availability of mutants, and ease of phenotyping of mutants of this organ makes it a model for understanding the mechanism of cell elongation and various biological events that participate in its control (Gendreau et al., 1997;Vandenbussche et al., 2005;Boron and Vissenberg, 2014).In nature, the development of germinated seeds starts under soil cover; the seedling undergoing skotomorphogenic growth has a very long hypocotyl, which allows quick attaining of light and de-etiolation after

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Section: Yeast Two-hybrid Growth Assay Reveals Interaction Between Shsupporting

confidence: 84%

Section: Role Of Sh1 In Lduvb-dependent Hypocotyl Elongation: a Modelmentioning

confidence: 99%

mentioning

confidence: 99%

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SHORT HYPOCOTYL 1 Encodes a SMARCA3-like Chromatin Remodeling Factor Regulating Elongation

Bo¹,

Wang²,

Pan³

et al. 2016

Plant Physiol.

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“…Plants sense UV-B radiation via the homodimeric UV-B photoreceptor UV Resistance Locus 8 (UVR8) (21)(22)(23) that is mainly localized in the cytosol (24). Absorption of UV-B photons by intrinsic tryptophan residues leads to UVR8 monomerization, interaction with the E3 ubiquitin ligase Constitutively Photomorphogenic 1 (COP1), accumulation in the nucleus, and changes in gene expression (19,(21)(22)(23)(24)(25)(26)(27)(28)(29). After photoreception, UVR8 returns to the homodimeric ground state by redimerization (30,31).…”

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confidence: 99%

UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii

Allorent

Lefebvre-Legendre

Chappuis

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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141

138

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Life on earth is dependent on the photosynthetic conversion of light energy into chemical energy. However, absorption of excess sunlight can damage the photosynthetic machinery and limit photosynthetic activity, thereby affecting growth and productivity. Photosynthetic light harvesting can be down-regulated by nonphotochemical quenching (NPQ). A major component of NPQ is qE (energy-dependent nonphotochemical quenching), which allows dissipation of light energy as heat. Photodamage peaks in the UV-B part of the spectrum, but whether and how UV-B induces qE are unknown. Plants are responsive to UV-B via the UVR8 photoreceptor. Here, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast.L ight is essential for photosynthesis, but absorption of excess light energy is detrimental. To avoid photodamage, photosynthetic light harvesting is regulated by nonphotochemical quenching (NPQ), which allows dissipation of harmful excess energy as heat through its qE (energy-dependent nonphotochemical quenching) component (1-6). Specialized members of the light harvesting complex (LHC) protein family, such as Photosystem II Subunit S (PSBS) in higher plants or members of the LHC Stress-Related (LHCSR) family in mosses and algae, are central to qE (7-11). Protonation of key residues in these proteins triggers qE in response to the acidification of the thylakoid lumen, which is coupled to photosynthetic electron transport (7, 9). Furthermore, the deepoxidation of violaxanthin to zeaxanthin, which is also activated by the acidification of the thylakoid lumen, enhances qE (12). In response to high levels of visible light, LHCSR3 protein accumulation is of major importance for qE capacity in Chlamydomonas reinhardtii (11). The induction of LHCSR3 expression under high light is thought to involve retrograde signaling, from the chloroplast to nuclear gene expression (13), and recent data show that the response is also dependent on the phototropin (PHOT) blue light photoreceptor (14).UV-B radiation is intrinsic to sunlight reaching the earth surface and is potentially damaging to living tissues. UV-B stress tolerance is induced through the specific activation of acclimation responses (15)(16)(17)(18)(19)(20). Plants sense UV-B radiation via the homodimeric UV-B photoreceptor UV Resistance Locus 8 (UVR8) (21-23) that is mainly localized in the cytosol ...

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“…Heat and UV-B signaling systems(Box et al, 2015;Hayes et al, 2017;Quint et al, 2016;Yin et al, 2016) are, respectively, shown in red and purple (online only). Heat and UV-B signaling systems(Box et al, 2015;Hayes et al, 2017;Quint et al, 2016;Yin et al, 2016) are, respectively, shown in red and purple (online only).…”

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confidence: 99%

Pivotal roles of environmental sensing and signaling mechanisms in plant responses to climate change

Bigot

Buges

Gilly

et al. 2018

Global Change Biology

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Climate change reshapes the physiology and development of organisms through phenotypic plasticity, epigenetic modifications, and genetic adaptation. Under evolutionary pressures of the sessile lifestyle, plants possess efficient systems of phenotypic plasticity and acclimation to environmental conditions. Molecular analysis, especially through omics approaches, of these primary lines of environmental adjustment in the context of climate change has revealed the underlying biochemical and physiological mechanisms, thus characterizing the links between phenotypic plasticity and climate change responses. The efficiency of adaptive plasticity under climate change indeed depends on the realization of such biochemical and physiological mechanisms, but the importance of sensing and signaling mechanisms that can integrate perception of environmental cues and transduction into physiological responses is often overlooked. Recent progress opens the possibility of considering plant phenotypic plasticity and responses to climate change through the perspective of environmental sensing and signaling. This review aims to analyze present knowledge on plant sensing and signaling mechanisms and discuss how their structural and functional characteristics lead to resilience or hypersensitivity under conditions of climate change. Plant cells are endowed with arrays of environmental and stress sensors and with internal signals that act as molecular integrators of the multiple constraints of climate change, thus giving rise to potential mechanisms of climate change sensing. Moreover, mechanisms of stress-related information propagation lead to stress memory and acquired stress tolerance that could withstand different scenarios of modifications of stress frequency and intensity. However, optimal functioning of existing sensors, optimal integration of additive constraints and signals, or memory processes can be hampered by conflicting interferences between novel combinations and novel changes in intensity and duration of climate change-related factors. Analysis of these contrasted situations emphasizes the need for future research on the diversity and robustness of plant signaling mechanisms under climate change conditions.

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COP1 is required for UV-B–induced nuclear accumulation of the UVR8 photoreceptor

Cited by 127 publications

References 52 publications

SHORT HYPOCOTYL 1 Encodes a SMARCA3-like Chromatin Remodeling Factor Regulating Elongation

SHORT HYPOCOTYL 1 Encodes a SMARCA3-like Chromatin Remodeling Factor Regulating Elongation

UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii

Pivotal roles of environmental sensing and signaling mechanisms in plant responses to climate change

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