Coordinated transcriptional regulation of the divinyl ether biosynthetic genes in tobacco by signal molecules related to defense

Fammartino, Alessandro; Verdaguer, Bertrand; Fournier, Joëlle; Tamietti, G.; Carbonne, Francis; Esquerré-Tugayé, Marie-Thérèse; Cardinale, Francesca

doi:10.1016/j.plaphy.2010.01.012

Cited by 21 publications

(8 citation statements)

References 47 publications

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“…To look for a potential elicitor activity, the various fractions were infiltrated into the leaves of transgenic tobacco plants expressing the uidA reporter gene (encoding the enzyme β -glucuronidase, GUS ) fused to the 2.3 kb regulatory promoter region of the tobacco lipoxygenase gene NtLOX1 (Fanmartino et al [24]). NtLOX1 is induced in response to elicitor and jasmonic acid treatment as well as during infection with an oomycete pathogen [25].…”

Section: Resultsmentioning

confidence: 99%

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Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

Jaulneau

Lafitte

Jacquet

et al. 2010

Journal of Biomedicine and Biotechnology

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131

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The industrial use of elicitors as alternative tools for disease control needs the identification of abundant sources of them. We report on an elicitor obtained from the green algae Ulva spp. A fraction containing most exclusively the sulfated polysaccharide known as ulvan-induced expression of a GUS gene placed under the control of a lipoxygenase gene promoter. Gene expression profiling was performed upon ulvan treatments on Medicago truncatula and compared to phytohormone effects. Ulvan induced a gene expression signature similar to that observed upon methyl jasmonate treatment (MeJA). Involvement of jasmonic acid (JA) in ulvan response was confirmed by detecting induction of protease inhibitory activity and by hormonal profiling of JA, salicylic acid (SA) and abscisic acid (ABA). Ulvan activity on the hormonal pathway was further consolidated by using Arabidopsis hormonal mutants. Altogether, our results demonstrate that green algae are a potential reservoir of ulvan elicitor which acts through the JA pathway.

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Section: Resultsmentioning

confidence: 99%

“…Active fractions were selected on the basis of their ability to induce, in transgenic tobacco, a reporter GUS gene construction under the control of the elicitor-inducible NtLOX1 gene promoter (Fanmartino et al [24]). The major recovered active compound was identified as ulvan, a polysaccharide specifically found in green algae.…”

Section: Discussionmentioning

confidence: 99%

Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

Jaulneau

Lafitte

Jacquet

et al. 2010

Journal of Biomedicine and Biotechnology

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131

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“…Ethylene was used as powerful anesthetic in surgery 33 and plant cells synthesize ether under pathogen attack or wounding. [34][35][36][37] Intriguingly, ethylene is produced in stressed plants 29,30 and is also abundantly synthesized during, and necessary for, fruit maturation. 31,32 Ethylene effects on plant roots are similar to those induced by halothane 38 and anesthetized plants also showed reduced chilling injuries.…”

Section: Stressed Plants Synthesize Anesthetics Ether and Ethylenementioning

confidence: 99%

Plant anesthesia supports similarities between animals and plants

Grémiaux¹,

Yokawa

Mancuso

et al. 2014

Plant Signaling & Behavior

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The French scientist Claude Bernard (1813-1878) is famous for his discoveries in physiology and for introducing rigorous experimental methods to medicine and biology. One of his major technical innovations was the use of chemicals in order to disrupt normal physiological function to test hypotheses. But less known is his conviction that the physiological functions of all living organisms rely on the same underlying principles. He hypothesized that similarly to animals, plants are also able to sense changes in their environment. He called this ability "sensitivity." In order to test his ideas, he performed anesthesia on plants and the results of these experiments were presented in 1878 in "Leçonssur les phénomènes de la vie communs aux animaux et aux végétaux." The phenomena described by Claude Bernard more than a century ago are not fully understood yet. Here, we present a short overview of anesthetic effects in animals and we discuss how anesthesia affects plant movements, seed germination, and photosynthesis. Surprisingly, these phenomena may have ecological relevance, since stressed plants generate anesthetics such as ethylene and ether. Finally, we discuss Claude Bernard's interpretations and conclusions in the perspective of modern plant sciences.

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“…Nicotiana tabacum P1Lox::GUS plants (Fammartino et al 2010) were grown in soil in a growth chamber (16 h light at 25°C, 8 h dark at 22°C). Fully expanded leaves of 5-week old N. tabacum P1Lox::GUS were infiltrated with either water as control, an extract of Phytophthora parasitica cell wall (P2) at 30 μg ml −1 (Roux et al 1994) or the green algal extracts at various dilultions.…”

Section: Gus Activitymentioning

confidence: 99%

An Ulva armoricana extract protects plants against three powdery mildew pathogens

et al. 2011

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The protective activity of a crude extract prepared from the green macroalga, Ulva armoricana, previously shown to induce plant defence responses, was evaluated on three plant species, common bean, grapevine and cucumber, cultivated in the greenhouse and inoculated with three powdery mildew pathogens Erysiphe polygoni, E. necator and Sphareotheca fuliginea respectively. Chemical analyses showed that the extract was enriched in ulvans, which are green algae polysaccharides essentially composed of uronic acid and sulphated rhamnose. Weekly applications were performed by spraying of the green algal extract at various dilutions on bean, grapevine and cucumber leaves. A significant effect (50% protection) was observed using a dilution corresponding to about 3 gl −1 dry matter and up to 90% reduction of symptom severity was obtained for the highest concentration (1/9 dilution, 6 gl −1 dry matter) for the three plant species. To study the natural variability of the protective activity, five extracts prepared from algae batches harvested at different year periods were evaluated. Although polysaccharide composition varied among batches, all extracts elicit a reporter gene regulated by a defence-gene promoter in a transgenic tobacco line, and protect cucumber plants against powdery mildew infection. Together, these data demonstrate that U. armoricana is a reproducible source of active compounds which can be used to efficiently protect crop plants against powdery mildew diseases.

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Coordinated transcriptional regulation of the divinyl ether biosynthetic genes in tobacco by signal molecules related to defense

Cited by 21 publications

References 47 publications

Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

Plant anesthesia supports similarities between animals and plants

An Ulva armoricana extract protects plants against three powdery mildew pathogens

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