1995
DOI: 10.1021/bi00008a015
View full text |Buy / Rent full text
|
Sign up to set email alerts
|

Abstract: It has been suggested that cooperative binding of estrogen receptor (ER) may, in part, be responsible for the synergistic activation of transcription of estrogen-responsive genes that contain multiple estrogen-response elements (EREs). Experiments described here show that estradiol-liganded ER (E2-ER) binds cooperatively to stereoaligned EREs that are surrounded by naturally occurring flanking sequences, such as an AT-rich region. In contrast, EREs lacking these sequences do not bind E2-ER cooperatively, regar… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

3
18
0
1

Year Published

1997
1997
2012
2012

Publication Types

Select...
5

Relationship

1
4

Authors

Journals

citations
Cited by 51 publications
(22 citation statements)
references
References 43 publications
(68 reference statements)
3
18
0
1
Order By: Relevance
“…In previous work, we quantitated the effect of ERE sequences, spacing, and the role of sequences flanking the ERE on the affinity and stoichiometry of liganded ER-ERE interaction in vitro (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). In sum, our results indicate a critical role for sequences flanking the ERE and the relative helix orientation of the EREs on ER binding affinity.…”
mentioning
confidence: 68%
“…In previous work, we quantitated the effect of ERE sequences, spacing, and the role of sequences flanking the ERE on the affinity and stoichiometry of liganded ER-ERE interaction in vitro (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). In sum, our results indicate a critical role for sequences flanking the ERE and the relative helix orientation of the EREs on ER binding affinity.…”
mentioning
confidence: 68%
“…The consensus ERE sequence is a 13-bp palindromic inverted repeat: 5Ј-GGTCAnnnTGACC-3Ј (9,29). However, in most ER target genes, the EREs do not match the consensus (1,30), and even half-sites can mediate activity, usually in association with Sp1 (16,28,42). The putative ERE found in the Flt1-T promoter is composed of only one functional half-site (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Many estrogen-regulated genes whose transcription is highly stimulated by E 2 contain AT-rich nucleotides immediately 3 adjacent to the ERE palindrome (Anolik et al 1995). To test the effect of ERE flanking sequences on ER binding affinity, a consensus AT-rich DNA sequence derived from the nucleotides immediately flanking the ERE in the 3 direction in the Xenopus vitellogenin A1 and A2 and the chicken apo-VLDLII gene promoters (Peale et al 1988) was added sequentially to the ERE palindrome.…”
Section: Effect Of At-rich Dna 3′ To the Ere On Er Binding Affinitymentioning
confidence: 99%