2011
DOI: 10.1073/pnas.1101569108
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Abstract: Convergent gene pairs with head-to-head configurations are widespread in both eukaryotic and prokaryotic genomes and are speculated to be involved in gene regulation. Here we present a unique mechanism of gene regulation due to convergent transcription from the antagonistic prgX/prgQ operon in Enterococcus faecalis controlling conjugative transfer of the antibiotic resistance plasmid pCF10 from donor cells to recipient cells. Using mathematical modeling and experimentation, we demonstrate that convergent trans… Show more

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Cited by 84 publications
(131 citation statements)
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“…ematical model incorporated the underlying genetic regulation of pCF10, which entails nonlinear interactions of sense:antisense transcripts within prgQ and prgX operons and interactions of PrgX with C and I (7,11,18) and is described in detail in Supporting Information. The parameter values were obtained from experimental results and from the literature (Table S2) (7).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…ematical model incorporated the underlying genetic regulation of pCF10, which entails nonlinear interactions of sense:antisense transcripts within prgQ and prgX operons and interactions of PrgX with C and I (7,11,18) and is described in detail in Supporting Information. The parameter values were obtained from experimental results and from the literature (Table S2) (7).…”
Section: Resultsmentioning
confidence: 99%
“…1) (5,6). The direct effect of C on pheromone responsive gene Q (prgQ) transcription is enhanced greatly by several co-as well as posttranscriptional mechanisms, whose cumulative effects cause the system to function as a bistable genetic switch (7). It also is noteworthy that pheromone induction increases the virulence of donor strains (8).…”
mentioning
confidence: 99%
“…A strain carrying pDM4 (Table S1), which contains a segment of pCF10 from P X −176 to +223, but does not include P Q or the prgX ORF (Fig. 1), produced abundant Anti-Q (15,16,18). The 3′ terminus of Anti-Q produced from this plasmid and from pCF10 was located at P X + 107, as determined by a 3′ RACE protocol.…”
Section: Resultsmentioning
confidence: 99%
“…Cells with the mutant Anti-Q allele were induced at a low level by small amounts of cCF10, whereas cells with a wild-type Anti-Q allele were not. Given that the pheromone induction system of pCF10 exhibits properties of a bistable switch (18), Anti-Q could reduce the sensitivity of the system to noise, either minor fluctuations in external cCF10 levels or stochastic fluxes in prgQ transcription. This would prevent unproductive mating responses.…”
Section: Discussionmentioning
confidence: 99%
“…[13], Bacillus subtilis [26], Vibrio cholerea [12], Chlamydia trachomatis [27], Psuedomonas aeruginosa [28], Psuedomonas syringae [29], Staphylococcus aureus [30], and Sinorhizobium meliloti [31]. Increasing knowledge and information of abundance of antisense genes has caused speculation that antisense transcription is an important hidden layer of regulation [16,[32][33][34].…”
mentioning
confidence: 99%