Convergent effects of growth factors, hormones, and fibronectin are necessary for the enterocyte differentiation of a colon adenocarcinoma cell line (HT29-D4)

Rognoni, J. B.; Pichard, Véronique; Honoré, Stéphane; Rigot, Véronique; Lehmann, Maxime; Roccabianca, Monique; Carles, Gérard; Luis, José; Marvaldi, Jacques; Briand, Claudette

doi:10.1007/s002580050255

Cited by 4 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neoplastic transformation of colonic epithelium is associated with disordered regulation of cellular differentiation and apoptosis (2,6). Numerous factors are involved in the control of intestinal epithelial cell differentiation, including growth factors, hormones, extracellular matrix proteins, vitamins, and luminal nutrients such as shortchain fatty acids (SCFAs) (2,(7)(8)(9)(10).…”

mentioning

confidence: 99%

Butyrate-Induced Differentiation of Colon Cancer Cells Is PKC and JNK Dependent

et al. 2005

View full text Add to dashboard Cite

Butyric acid, a short-chain fatty acid physiologically present in human large gut, is derived from bacterial fermentation of complex carbohydrates. It has been shown to reduce the growth and motility of colon cancer cell lines and to induce cell differentiation and apoptosis. Apoptosis is considered a result of normal colonocyte terminal differentiation in vivo. The aim of this study was to characterize the cellular mechanisms regulating differentiation of colon cancer cells stimulated with sodium butyrate (NaB). The two human colon cancer cell lines Caco-2 and HT-29 were treated with NaB at physiologically relevant concentrations. Alkaline phosphatase (ALP) activity, a marker of colonocyte differentiation, was increased 48 hr after treatment with 1 mM NaB. Higher doses of NaB (5 and 10 mM) induced apoptosis of the cells and failed to stimulate the colonocyte differentiation. Therefore, we assumed that butyrate augments cell differentiation and induces apoptosis, acting via various intracellular mechanisms, and butyrate-mediated programmed cell death cannot be considered a consequence of colonocyte terminal differentiation. The effect of NaB on ALP activity was significantly attenuated in the presence of inhibitors of protein kinase C and JNK. Inhibition of MEK-ERK signal transduction pathways augmented the impact of butyrate on colonocyte differentiation. These results suggest that butyrate could influence the colonocyte differentiation via modulation of the activity of cellular protein kinases and signal transduction.

show abstract

mentioning

confidence: 99%

Butyrate-Induced Differentiation of Colon Cancer Cells Is PKC and JNK Dependent

et al. 2005

View full text Add to dashboard Cite

show abstract

“…There are studies on thyroid hormone indicating increased intestinal differentiation, brush border enzyme activity, and differentiation factor activities in adult rats and other adult species [32][33][34][35]. Thyroid hormone acts on proliferation by structural and functional maturation of the mammalian small intestine, mitochondrial pathways, and several protein gene activation mechanisms [36][37][38][39]. There are limited numbers of studies that have been performed in adult rats showing increased enterocyte differentiation, increased intestinal brush border enzyme gene expression, with similar thyroid replacement doses used in the present study [32,33].…”

Section: Discussionmentioning

confidence: 98%

Effects of Thyroid Hormone on the Adaptation in Short Bowel Syndrome

Yüksel¹,

Tatlıcıoğlu²,

Poyraz³

et al. 2009

Journal of Surgical Research

View full text Add to dashboard Cite

“…In the colon adenocarcinoma cell line HT29-D4, albumin addition induced cell growth accompanied with a low level of differentiation (20). However, in human MCF-7 breast cancer cells, proliferation was inhibited by albumin supplementation (21,31).…”

Section: Discussionmentioning

confidence: 98%

“…The poor solubility of fatty acids in water impedes their administration in cell culture medium. Supplementation of albumin in the culture medium may overcome this problem; however, albumin may artificially perturb the effects of fatty acids (6,20,21). Another interfering factor is that supplemented albumin may alter the availability of fatty acids to normal and tumor cells (22,23).…”

mentioning

confidence: 98%

Optimization of methods and treatment conditions for studying effects of fatty acids on cell growth

Tronstad

Berge

Flindt

et al. 2001

Lipids

View full text Add to dashboard Cite

Antiproliferative properties of molecular regulators of lipid metabolism have been increasingly studied during recent years. Discussion is ongoing concerning optimal treatment conditions and assays used for monitoring proliferation and cytotoxicity. The objective of the present work was to optimize methods and treatment conditions used for studying antiproliferative effects of fatty acids and analogs, represented by palmitic acid (PA) and the beta-oxidation-restricted fatty acid analog tetradecylthioacetic acid (TTA), in rat (BT4Cn) and human (D54Mg and GaMg) glioma cell lines. Changes in [3H]thymidine incorporation preceded changes in cell number in TTA-treated glioma cell cultures, and the growth inhibition was more significantly expressed by [3H]thymidine incorporation than cell number. Addition of bovine serum albumin decreased cellular fatty acid uptake and reduced the effects of TTA and PA on [3H]thymidine incorporation. Determination of the antiproliferative effect of TTA in BT4Cn cells by MTT conversion and [3H]thymidine incorporation yielded concordant results. TTA-mediated reduction in cell number corresponded to reduction in cellular protein and total DNA content in BT4Cn cells. Reduced growth potential in TTA-treated multicellular D54Mg and GaMg spheroids supported the findings from monolayer cultures. In conclusion, cell density, treatment period, fatty acid administration, and methods for growth determination may profoundly influence the outcome of cell growth experiments. Thus, experimental conditions should be carefully controlled when performing cell growth experiments, and effects on cell growth should preferably be confirmed by different methods.

show abstract

Convergent effects of growth factors, hormones, and fibronectin are necessary for the enterocyte differentiation of a colon adenocarcinoma cell line (HT29-D4)

Cited by 4 publications

References 0 publications

Butyrate-Induced Differentiation of Colon Cancer Cells Is PKC and JNK Dependent

Butyrate-Induced Differentiation of Colon Cancer Cells Is PKC and JNK Dependent

Effects of Thyroid Hormone on the Adaptation in Short Bowel Syndrome

Optimization of methods and treatment conditions for studying effects of fatty acids on cell growth

Contact Info

Product

Resources

About