Convergence of nociceptive information in the forebrain of female rats: Reproductive organ response variations with stage of estrus

Chadha, Harpreet K.; Hubscher, Charles H.

doi:10.1016/j.expneurol.2007.11.010

Cited by 20 publications

(19 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in female rats (5,23,24,56). Quantifying the serum levels of estradiol in the intact female rat just before electrophysiological study of acutely isolated myelinated Ah-type VGN may help determine whether the excitability of this unique subtype of vagal afferents from intact female rat is associated with the phase of the estrous cycle.…”

Section: Discussionmentioning

confidence: 99%

17β-Estradiol restores excitability of a sexually dimorphic subset of myelinated vagal afferents in ovariectomized rats

Qiao

et al. 2009

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Qiao GF, Li BY, Lu YJ, Fu YL, Schild JH. 17␤-Estradiol restores excitability of a sexually dimorphic subset of myelinated vagal afferents in ovariectomized rats. Am J Physiol Cell Physiol 297: C654 -C664, 2009. First published July 1, 2009 doi:10.1152/ajpcell.00059.2009.-We recently identified a myelinated vagal afferent subpopulation (Ah type) far more prevalent in female than male rats and showed that this difference extends to functionally specific visceral sensory afferents, baroreceptors of the aortic arch. Excitability of myelinated Ah-type afferents is markedly reduced after ovariectomy (OVX). Here we tested the hypothesis that 17␤-estradiol can selectively restore excitability of these sex-specific vagal afferents. Acutely isolated vagal afferent neurons (VGN) from intact and OVX adult female rats were used with patch-clamp technique and current-clamp protocols to assess the effect of acute application of 17␤-estradiol on neuronal excitability. At over physiologically relevant 17␤-estradiol concentrations for rat (1-10 nM) excitability of myelinated Ah-type vagal afferents is restored to discharge frequencies comparable to those in intact females, albeit with some interesting differences related to burst and sustained patterns of neuronal discharge. Restoration of excitability occurs within 3 min of hormone application and is stereo specific, because 1,000 nM 17␣-estradiol fails to alter excitability. Furthermore, activation of G protein-coupled estrogen receptor GPR30 with highly selective agonist G-1 similarly restores excitability of Ah-type afferents. The effectiveness of 17␤-estradiol and G-1 is completely eliminated by application of high-affinity estrogen receptor ligand ICI-182,780. 17␤-Estradiol conjugated with BSA is ϳ70% as effective as 17␤-estradiol alone in restoring Ah-type VGN excitability. These data support our conclusions that the cellular mechanisms leading to rapid restoration of neuronal excitability of myelinated Ah-type VGN after OVX occur, at least in part, via membrane-bound estrogen receptors. We contend that recovery of high-frequency discharge at physiologically relevant 17␤-estradiol concentrations implies that this unique subtype of low-threshold myelinated vagal afferent may account for some of the sex-related differences in visceral organ system function. Sex differences in cardiovascular and gastrointestinal function and the potential role of GPR30 in modulation of sex-specific myelinated Ah-type vagal afferents are discussed. sensory afferent; visceral afferent; estrogen receptor; GPR30; ovariectomy VISCERAL AFFERENTS with cell bodies in the vagal ganglia innervate a functionally diverse range of organs associated with the cardiovascular, gastrointestinal, and pulmonary systems. Vagal afferent mediation of the reflexogenic properties of the autonomic nervous system (ANS) in addition to visceral sensation is well recognized (for review see Ref. 55). Because the peripheral sensory terminals can be influenced by a wide range of endogenous chemicals released as a consequenc...

show abstract

Section: Discussionmentioning

confidence: 99%

17β-Estradiol restores excitability of a sexually dimorphic subset of myelinated vagal afferents in ovariectomized rats

Qiao

et al. 2009

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

“…In previous studies using female rats, neuronal responses to pelvic reproductive organ stimulation were found to vary with stage of estrus as a consequence of levels of estradiol and not progesterone. These findings were demonstrated for neurons present at different regions of the neural axis, including the preoptic area of the hypothalamus and bed nucleus of the stria terminalis (Chadha and Hubscher 2008), the thalamus (Reed et al 2009), and the MRF (Hubscher 2006). Responses to cervix stimulation at low pressures were consistent with low and not high serum levels of ovarian hormones in the present study (Chadha and Hubscher 2008;Hubscher 2006;Reed et al 2009).…”

Section: Discussionmentioning

confidence: 95%

“…A tungsten microelectrode with Ϸ7 Ϯ 1 M⍀ impedance (Fredrich Haer, Bowdoinham, ME), coated with fluorescent dye (DiI; Invitrogen, Carlsbad, CA) (Chadha and Hubscher 2008;Massey et al 2006), was lowered from the dorsal surface of the brain stem with a motorized drive (Fredrich Haer) into the MRF. Stereotaxic coordinates were 2,700 -3,300 m rostral to obex and 300 -1,400 m lateral to midline on both sides of the brain stem.…”

Section: Animalsmentioning

confidence: 99%

See 1 more Smart Citation

Convergence and cross talk in urogenital neural circuitries

Hubscher

Gupta

Brink

2013

Journal of Neurophysiology

View full text Add to dashboard Cite

Hubscher CH, Gupta DS, Brink TS. Convergence and cross talk in urogenital neural circuitries. J Neurophysiol 110: 1997J Neurophysiol 110: -2005J Neurophysiol 110: , 2013. First published August 7, 2013; doi:10.1152/jn.00297.2013.-Despite common comorbidity of sexual and urinary dysfunctions, the interrelationships between the neural control of these functions are poorly understood. The medullary reticular formation (MRF) contributes to both mating/arousal functions and micturition, making it a good site to test circuitry interactions. Urethane-anesthetized adult Wistar rats were used to examine the impact of electrically stimulating different nerve targets [dorsal nerve of the penis (DNP) or clitoris (DNC); L 6 /S 1 trunk] on responses of individual extracellularly recorded MRF neurons. The effect of bladder filling on MRF neurons was also examined, as was stimulation of DNP on bladder reflexes via cystometry. In total, 236 MRF neurons responded to neurostimulation: 102 to DNP stimulation (12 males), 64 to DNC stimulation (12 females), and 70 to L 6 /S 1 trunk stimulation (12 males). Amplitude thresholds were significantly different at DNP (15.0 Ϯ 0.6 A), DNC (10.5 Ϯ 0.7 A), and L 6 /S 1 trunk (54.2 Ϯ 4.6 A), whereas similar frequency responses were found (max responses near 30 -40 Hz). In five males, filling/voiding cycles were lengthened with DNP stimulation (11.0 Ϯ 0.9 A), with a maximal effective frequency plateau beginning at 30 Hz. Bladder effects lasted ϳ2 min after DNP stimulus offset. Many MRF neurons receiving DNP/DNC input responded to bladder filling (35.0% and 68.3%, respectively), either just before (43%) or simultaneously with (57%) the voiding reflex. Taken together, MRFevoked responses with neurostimulation of multiple nerve targets along with different responses to bladder infusion have implications for the role of MRF in multiple aspects of urogenital functions.

show abstract

“…49,51,52 Thalamic electrode tracks were made in parallel rows, 500 μm apart, and were located between −2.04 and −3.30 mm caudal to bregma and 1.2 and 3.8 mm lateral to midline. 49,53 Recording began at 4 mm below the surface of the cortex and ended at 7.5 mm.…”

Section: Electrophysiologymentioning

confidence: 99%

Effect of Spinal Manipulation Thrust Duration on Trunk Mechanical Activation Thresholds of Nociceptive Specific Lateral Thalamic Neurons

ReedWilliam

SozioRandall

PickarJoel

et al. 2014

The Journal of Alternative and Complementary Medicine

View full text Add to dashboard Cite

Objective-The objective of this preliminary study was to determine if high-velocity, lowamplitude spinal manipulation (HVLA-SM) thrust duration alters mechanical trunk activation thresholds of nociceptive-specific (NS) lateral thalamic neurons.Methods-Extracellular recordings were obtained from 18 NS neurons located in 2 lateral thalamic nuclei (ventrolateral [n = 12] and posterior [n = 6]) in normal anesthetized Wistar rats. Response thresholds to electronic von Frey anesthesiometer (rigid tip) mechanical trunk stimuli applied in 3 lumbar directions (dorsal-ventral, 45° caudal, and 45° cranial) were determined before and immediately after the delivery of 3 HVLA-SM thrust durations (time control 0, 100, and 400 milliseconds). Mean changes in mechanical trunk activation thresholds were compared using a mixed model analysis of variance.Results-High-velocity, low-amplitude spinal manipulation duration did not significantly alter NS lateral thalamic neurons' mechanical trunk responses to any of the 3 directions tested with the anesthesiometer.Copyright © 2014 by National University of Health Sciences.Submit requests for reprints to: William R Reed, DC, PhD, 741 Brady St, Davenport, IA 52803. (william.reed@palmer.edu). Potential Conflicts of Interest No conflicts of interest were reported for this study Contributorship Information Concept development (provided idea for the research): W.R.R. Design (planned the methods to generate the results): W.R.R., J.G.P., R.S.S. Supervision (provided oversight, responsible for organization and implementation, writing of the manuscript): W.R.R. Data collection/processing (responsible for experiments, patient management, organization, or reporting data): W.R.R., R.S.S. Analysis/interpretation (responsible for statistical analysis, evaluation, and presentation of the results): W.R.R., J.G.P., S.M.O., R.S.S. Literature search (performed the literature search): W.R.R., S.M.O., R.S.S. Writing (responsible for writing a substantive part of the manuscript): W.R.R., S.M.O. Critical review (revised manuscript for intellectual content, this does not relate to spelling and grammar checking): W.R.R., J.G.P., S.M.O., R.S.S.The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptConclusions-This study is the first to examine the effect of HVLA-SM thrust duration on NS lateral thalamic mechanical response thresholds. High-velocity, low-amplitude spinal manipulation thrust duration did not affect mechanical trunk thresholds. Key Indexing TermsManipulation; Spinal; Thalamus; Nociceptive Neuron; Lumbar Vertebrae; ChiropracticSpinal manipulation has been shown to be effective in treating neck and low back pain. [1][2][3] Not only are the neurophysiological mechanisms by which this occurs unknown, but a lack of knowledge also exists regarding the effects of clinician-controlled mechanical application parameters o...

show abstract

Convergence of nociceptive information in the forebrain of female rats: Reproductive organ response variations with stage of estrus

Cited by 20 publications

References 95 publications

17β-Estradiol restores excitability of a sexually dimorphic subset of myelinated vagal afferents in ovariectomized rats

17β-Estradiol restores excitability of a sexually dimorphic subset of myelinated vagal afferents in ovariectomized rats

Convergence and cross talk in urogenital neural circuitries

Effect of Spinal Manipulation Thrust Duration on Trunk Mechanical Activation Thresholds of Nociceptive Specific Lateral Thalamic Neurons

Contact Info

Product

Resources

About