Controlling loading and optical properties of gold nanoparticles on liposome membranes

Sau, Tapan K.; Urban, Alexander S.; Dondapati, Srujan Kumar; Федорук, М. П.; Horton, Margaret R.; Rogach, Andrey L.; Stefani, Fernando D.; Rädler, Joachim O.; Feldmann, Jochen

doi:10.1016/j.colsurfa.2009.04.014

Cited by 34 publications

(24 citation statements)

References 42 publications

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“…Therefore, there is the urgency to determine how the size, charge, and surface chemistry of nanoparticles influence their functional mechanism and their cytotoxicity. [45][46][47] In this study, nanoparticle -membrane interaction was characterized in order to provide fundamental understanding of the interaction between nanoparticles and cellular systems, and to provide guidance in the design and development of safe nanoparticles for biological applications.…”

Section: Nanoparticle -Membrane Interactionmentioning

confidence: 99%

Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption

Bothun

2014

Analyst

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Physical disruption of cellular membranes arising from interactions with engineered nanoparticles is an important, but poorly understood aspect of nanotoxicology and nanomedicine. Model cellular membranes (i.e. lipid bilayers) can be used to identify interaction mechanisms, and most studies have largely focused on lipid bilayers supported on solid planar or spherical substrates. While useful and informative, these systems do not accurately represent an intact cell membrane because they restrict the elastic motion of the bilayer and the capacity for mechanical changes.Free standing bilayers are preferred, but add complexity. Given the importance of nanoparticle-membrane interactions in nanotoxicology and nanomedicine, and the vast range in nanoparticle composition, size, shape, and surface functionalization, there is a need to develop techniques that can rapidly and inexpensively analyze the membranenanoparticle activity by using free standing or unsupported membranes.This work develops a centrifugation-based assay that can analyze the membrane-nanoparticle activity as a function of nanoparticle surface functionalization, membrane lipid composition, and monovalent salt concentration (NaCl). Free standing, unsupported vesicles were used to gain relevant information on elastic membrane deformation and vesicle destabilization due to nanoparticle binding. Silver nanoparticles were chosen due to their widespread biological applications and surface plasmon resonance (SPR) properties. UV-vis based centrifugation assay, coupled with cryo-TEM and DLS analysis, was proposed to screen nanoparticle-membrane interactions; silver nanoparticles binding ratio RSPR was calculated as a function of Ag nanoparticle coating and vesicle composition. Study showed that strong electrostatic attraction led to significant sedimentation, vesicle / membrane disruption and higher RSPR value; in contrast, systems that exhibited weak or no electrostatic attraction did not show significant sedimentation, membrane disruption or high RSPR value. The centrifugation assay provides a rapid and straightforward way to screen nanoparticlemembrane interactions.iv ACKNOWLEDGMENTS

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Section: Nanoparticle -Membrane Interactionmentioning

confidence: 99%

Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption

Bothun

2014

Analyst

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“…This is an important issue in the context of using NPs in nanomedicine but also in the related eld of nanotoxicology. Experimental [11][12][13][14][15] and theoretical [16][17][18][19][20][21][22][23][24] investigations on those mixed systems have led to the description of different scenarios such as the encapsulation of the NPs within the vesicle or within the phospholipid membrane (for very small and hydrophobic NPs), or simply an attachment to the membrane. Focusing on the latter, the adsorption of NPs has been found to have an important inuence on the deformation and poration of lipid membranes 25,26 but also to act as stabilizer for liposome dispersions [27][28][29] introducing an electrostatic repulsion between the nanoparticle/vesicle complexes.…”

Section: Introductionmentioning

confidence: 99%

Softening of phospholipid membranes by the adhesion of silica nanoparticles – as seen by neutron spin-echo (NSE)

Hoffmann

Michel

Sharp³

et al. 2014

Nanoscale

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The interactions between nanoparticles and vesicles are of significant interest both from a fundamental as well as from a practical point of view, as vesicles can serve as a model system for cell membranes. Accordingly the effect of nanoparticles that bind to the vesicle bilayer is very important with respect to understanding their biological impact and also may shed some light on the mechanisms behind the effect of nanotoxicity. In this study we have investigated the influence of small adsorbed silica nanoparticles (SiNPs) on the structure of zwitterionic DOPC vesicles. By a combination of SANS, cryo-TEM, and DLS, we observed that the SiNPs are bound to the outer vesicle surface without significantly affecting the vesicle structure. Most interestingly, by means of neutron spin-echo (NSE) local bilayer fluctuations were studied and one finds a small but marked decrease of the membrane rigidity upon binding of the nanoparticles. This surprising finding may be a relevant aspect for the further understanding of the effects that nanoparticles have on phospholipid bilayers.

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“…The surface decoration of liposomes with NPs may also be achieved through the selective reduction and deposition of metal atoms at the liposome surface . Rengan et al.…”

Section: Methods and Effects Of Incorporating Inorganic Nps Into Drugmentioning

confidence: 99%

Stimuli‐Responsive Release of Antimicrobials Using Hybrid Inorganic Nanoparticle‐Associated Drug‐Delivery Systems

Moorcroft

Jayne

Evans

et al. 2018

Macromolecular Bioscience

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Recently, the combination of metallic nanoparticles (NPs) of Au, Ag, Fe2O3, and Fe3O4 with traditional soft matter drug‐delivery systems has emerged as a promising strategy to achieve site‐specific and controlled release of antimicrobial agents. By harnessing the plasmonic and magnetic properties of inorganic NPs, the disruption of antibiotic‐loaded liposomes, polymersomes, and hydrogels can be remotely triggered by mechanisms such as photo‐ and magneto‐thermal effects, microbubble cavitation, magnetic positioning, and pH‐changes, hence offering significant advantages in improving antibacterial efficacy, reducing side effects, and in overcoming antimicrobial resistance. This review highlights the latest development of stimuli‐responsive antibiotic delivery systems incorporating inorganic NPs. The methods employed for preparation of hybrid inorganic NP‐associated drug‐delivery systems and the effects this has upon the system are discussed. Finally, a detailed exposition of the NP‐mediated triggering mechanisms is provided and pertinent examples of their use in antimicrobial applications are presented.

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Controlling loading and optical properties of gold nanoparticles on liposome membranes

Cited by 34 publications

References 42 publications

Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption

Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption

Softening of phospholipid membranes by the adhesion of silica nanoparticles – as seen by neutron spin-echo (NSE)

Stimuli‐Responsive Release of Antimicrobials Using Hybrid Inorganic Nanoparticle‐Associated Drug‐Delivery Systems

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